MTP抗体 (D-4) 推荐用于 mouse, rat 和 human 来源的MTP WB, IP, IF, IHC(P) 和 ELISA检测

MTP 抗体 (D-4): sc-515743

MTP抗体 (D-4) is rated 4.0 out of 5 by 1.
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说明书

    • MTP抗体(D-4)是小鼠单克隆IgG2b (kappa轻链),规格为200 µg/ml
    • 免疫human来源MTP位于N-terminus 的1-300氨基酸
    • 推荐用于 mouse, rat 和 human 来源的MTP WB, IP, IF, IHC(P) 和 ELISA检测
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How much sample do you recommend loading on the gel for Western blot detection using MTP (D-4): sc-515743?

Asked by: Professor Griffin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Works in mice I received a 10 ug/mL sample and used it for western blotting in mouse tissue. It worked well when diluted 1:1000. I attached a photo to show my results. My colleague tested the antibody in HUH-7 cells, and did not get a band when western blotting using the same 1:1000 dilution.
Date published: 2019-03-22
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