MRTF-A/MKL1 Antibody (G-8): sc-390324
- MRTF-A/MKL1 Antibody (G-8) is a mouse monoclonal IgG2a κ, cited in 25 publications, provided at 200 µg/ml
- raised against amino acids 761-900 mapping near the C-terminus of MRTF-A of human origin
- Anti-MRTF-A/MKL1 Antibody (G-8) is recommended for detection of MRTF-A of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-MRTF-A/MKL1 Antibody (G-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MRTF-A/MKL1 Antibody (G-8) for WB applications. These reagents are now offered in bundles with MRTF-A/MKL1 Antibody (G-8) (see ordering information below).
QUICK LINKS
MRTF-A Antibody (G-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects MRTF-A in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-MRTF-A antibody (G-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MRTF-A, also known as myocardin-related transcription factor A, plays a crucial role in regulating gene expression in response to growth factors, particularly in muscle cells. MRTF-A primarily localizes in the nucleus, where MRTF-A interacts with serum response factor (SRF) to enhance transcriptional activity of muscle-specific genes. This nuclear localization enables MRTF-A to effectively modulate the expression of genes involved in muscle development and cardiovascular function, influencing processes such as myogenesis and vascular smooth muscle differentiation. MRTF-A and SRF interaction is essential for cardiovascular system formation during vertebrate development, highlighting MRTF-A′s importance in both normal physiological processes and potential pathological conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MRTF-A/MKL1 Antibody (G-8) References:
- Potentiation of serum response factor activity by a family of myocardin-related transcription factors. | Wang, DZ., et al. 2002. Proc Natl Acad Sci U S A. 99: 14855-60. PMID: 12397177
- Sphingosine 1-phosphate stimulates smooth muscle cell differentiation and proliferation by activating separate serum response factor co-factors. | Lockman, K., et al. 2004. J Biol Chem. 279: 42422-30. PMID: 15292266
- Identification of direct serum-response factor gene targets during Me2SO-induced P19 cardiac cell differentiation. | Zhang, SX., et al. 2005. J Biol Chem. 280: 19115-26. PMID: 15699019
- Acute myeloid leukemia-associated Mkl1 (Mrtf-a) is a key regulator of mammary gland function. | Sun, Y., et al. 2006. Mol Cell Biol. 26: 5809-26. PMID: 16847333
- The SRE: a growth factor responsive transcriptional regulator. | Treisman, R. 1990. Semin Cancer Biol. 1: 47-58. PMID: 2133110
- The role of the MRTF-A/SRF pathway in ocular fibrosis. | Yu-Wai-Man, C., et al. 2014. Invest Ophthalmol Vis Sci. 55: 4560-7. PMID: 25056592
- Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-fos serum response element. | Norman, C., et al. 1988. Cell. 55: 989-1003. PMID: 3203386
- Actin-microtubule cytoskeletal interplay mediated by MRTF-A/SRF signaling promotes dilated cardiomyopathy caused by LMNA mutations. | Le Dour, C., et al. 2022. Nat Commun. 13: 7886. PMID: 36550158
- An MRTF-A-ZEB1-IRF9 axis contributes to fibroblast-myofibroblast transition and renal fibrosis. | Zhao, Q., et al. 2023. Exp Mol Med. 55: 987-998. PMID: 37121967
- MRTF-A gain-of-function in mice impairs homeostatic renewal of the intestinal epithelium. | Singh, AK., et al. 2023. Cell Death Dis. 14: 639. PMID: 37770456
- Gas6-Axl Signaling Induces SRF/MRTF-A Gene Transcription via MICAL2. | Lundquist, MR. and Jaffrey, SR. 2023. Genes (Basel). 14: PMID: 38137053
- Functional analysis of a growth factor-responsive transcription factor complex. | Hill, CS., et al. 1993. Cell. 73: 395-406. PMID: 8477450
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRTF-A/MKL1 Antibody (G-8) | sc-390324 | 200 µg/ml | $322.00 | |||
MRTF-A/MKL1 Antibody (G-8): m-IgG Fc BP-HRP Bundle | sc-530125 | 200 µg Ab; 10 µg BP | $361.00 | |||
MRTF-A/MKL1 Antibody (G-8): m-IgGκ BP-HRP Bundle | sc-523489 | 200 µg Ab, 40 µg BP | $361.00 | |||
MRTF-A/MKL1 Antibody (G-8): m-IgG2a BP-HRP Bundle | sc-547439 | 200 µg Ab; 10 µg BP | $361.00 | |||
MRTF-A/MKL1 Antibody (G-8) AC | sc-390324 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MRTF-A/MKL1 Antibody (G-8) HRP | sc-390324 HRP | 200 µg/ml | $322.00 | |||
MRTF-A/MKL1 Antibody (G-8) FITC | sc-390324 FITC | 200 µg/ml | $336.00 | |||
MRTF-A/MKL1 Antibody (G-8) PE | sc-390324 PE | 200 µg/ml | $349.00 | |||
MRTF-A/MKL1 Antibody (G-8) Alexa Fluor® 488 | sc-390324 AF488 | 200 µg/ml | $364.00 | |||
MRTF-A/MKL1 Antibody (G-8) Alexa Fluor® 546 | sc-390324 AF546 | 200 µg/ml | $364.00 | |||
MRTF-A/MKL1 Antibody (G-8) Alexa Fluor® 594 | sc-390324 AF594 | 200 µg/ml | $364.00 | |||
MRTF-A/MKL1 Antibody (G-8) Alexa Fluor® 647 | sc-390324 AF647 | 200 µg/ml | $364.00 | |||
MRTF-A/MKL1 Antibody (G-8) Alexa Fluor® 680 | sc-390324 AF680 | 200 µg/ml | $364.00 | |||
MRTF-A/MKL1 Antibody (G-8) Alexa Fluor® 790 | sc-390324 AF790 | 200 µg/ml | $364.00 |