Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
MRTF-A/MKL1 Antibody (G-8) - Western Blotting - Image 393573
MRTF-A 抗体 (G-8) HRP: sc-390324 HRP. HeLa (A) 和 A549 (B) 核提取物以及 Hep G2 (C), NIH/3T3 (D) 和 HeLa (E) 全细胞裂解液中 MRTF-A 表达的直接 Western 印迹分析.
MRTF-A/MKL1 Antibody (G-8) - Western Blotting - Image 393573
MRTF-A 抗体 (G-8) HRP: sc-390324 HRP. HeLa (A) 和 A549 (B) 核提取物以及 Hep G2 (C), NIH/3T3 (D) 和 HeLa (E) 全细胞裂解液中 MRTF-A 表达的直接 Western 印迹分析.
MRTF-A/MKL1 Antibody (G-8) - Western Blotting - Image 353665
MRTF-A 抗体 (G-8): sc-390324. HeLa (A), Hep G2 (B), HUV-EC-C (C) 和 NIH/3T3 (D) 全细胞裂解液及大鼠脑组织提取物 (E) 中 MRTF-A 表达的 Western 印迹分析.
MRTF-A/MKL1 Antibody (G-8) - Western Blotting - Image 308177
MRTF-A 抗体 (G-8): sc-390324. NIH/3T3 (A) 和 A549 (B) 核提取物以及 HeLa(C) 和 NIH/3T3 (D) 全细胞裂解液中 MRTF-A 表达的 Western 印迹分析.
MRTF-A 抗体 (G-8) HRP: sc-390324 HRP. HeLa (A) 和 A549 (B) 核提取物以及 Hep G2 (C), NIH/3T3 (D) 和 HeLa (E) 全细胞裂解液中 MRTF-A 表达的直接 Western 印迹分析.

MRTF-A/MKL1 抗体 (G-8): sc-390324

4.7(6)
寫評論提問

说明书
  • MRTF-A/MKL1抗体(G-8)是小鼠单克隆IgG2a κ, 在25篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列761-900位于human物种的MRTF-A的C-terminus附近
  • 抗-MRTF-A/MKL1 抗体 (G-8) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 MRTF-A
  • 抗MRTF-A/MKL1抗体（G-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MRTF-A/MKL1 (G-8): sc-390324的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是MRTF-A/MKL1 Antibody (G-8) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与MRTF-A/MKL1 Antibody (G-8) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

MRTF抗体

MRTF-A/MKL1抗体（G-8）是一种IgG2a κ型小鼠单克隆MRTF-A抗体，可通过WB、IP、IF和ELISA技术检测小鼠、大鼠和人的MRTF-A蛋白。MRTF-A/MKL1抗体（G-8）既有非偶联的抗MRTF-A抗体形式，也有多种偶联形式的抗MRTF-A抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。血清反应因子（SRF）是一种转录因子，它与血清反应元件（SRE）结合，SRE是一种介导许多细胞基因对生长刺激的瞬时反应的序列。SRF调节几种肌肉基因对生长因子的瞬时反应，并募集辅助成肌因子以激活这些肌肉基因。SRF对于脊椎动物中胚层的形成至关重要，中胚层是心血管系统的起源。心肌素与心肌细胞中的SRF结合，激活心肌启动子。心肌素相关转录因子A（MRTF-A）与SRF相互作用并刺激其转录活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MRTF-A/MKL1 抗体 (G-8) 参考文献:

  1. 心肌蛋白相关转录因子家族对血清反应因子活性的增效作用  |  Wang, DZ., et al. 2002. Proc Natl Acad Sci U S A. 99: 14855-60. PMID: 12397177
  2. 1- 磷酸肾上腺素通过激活独立的血清反应因子辅助因子，刺激平滑肌细胞分化和增殖。  |  Lockman, K., et al. 2004. J Biol Chem. 279: 42422-30. PMID: 15292266
  3. 鉴定 Me2SO 诱导 P19 心细胞分化过程中血清反应因子的直接基因靶点  |  Zhang, SX., et al. 2005. J Biol Chem. 280: 19115-26. PMID: 15699019
  4. 急性髓性白血病相关 Mkl1（Mrtf-a）是乳腺功能的关键调节因子。  |  Sun, Y., et al. 2006. Mol Cell Biol. 26: 5809-26. PMID: 16847333
  5. SRE：一种生长因子反应性转录调节器。  |  Treisman, R. 1990. Semin Cancer Biol. 1: 47-58. PMID: 2133110
  6. MRTF-A/SRF通路在眼部纤维化中的作用。  |  Yu-Wai-Man, C., et al. 2014. Invest Ophthalmol Vis Sci. 55: 4560-7. PMID: 25056592
  7. 编码 SRF（一种与 c-fos 血清反应元件结合的转录因子）的 cDNA 克隆的分离和特性。  |  Norman, C., et al. 1988. Cell. 55: 989-1003. PMID: 3203386
  8. MRTF-A/SRF信号介导的肌动蛋白-微管细胞骨架相互作用促进了由LMNA突变引起的扩张型心肌病。  |  Le Dour, C., et al. 2022. Nat Commun. 13: 7886. PMID: 36550158
  9. MRTF-A-ZEB1-IRF9轴促进成纤维细胞-肌成纤维细胞转化和肾纤维化。  |  Zhao, Q., et al. 2023. Exp Mol Med. 55: 987-998. PMID: 37121967
  10. 小鼠的MRTF-A功能增益会损害肠上皮细胞的同源更新。  |  Singh, AK., et al. 2023. Cell Death Dis. 14: 639. PMID: 37770456
  11. Gas6-Axl 信号通过 MICAL2 诱导 SRF/MRTF-A 基因转录。  |  Lundquist, MR. and Jaffrey, SR. 2023. Genes (Basel). 14: PMID: 38137053
  12. 生长因子响应转录因子复合物的功能分析。  |  Hill, CS., et al. 1993. Cell. 73: 395-406. PMID: 8477450

订购信息

产品名称产品编号规格价格数量收藏夹

MRTF-A/MKL1 抗体 (G-8)

sc-390324
200 µg/ml
$316.00

MRTF-A/MKL1 (G-8): m-IgG Fc BP-HRP 套装

sc-530125
200 µg Ab; 10 µg BP
$354.00

MRTF-A/MKL1 (G-8): m-IgGκ BP-HRP 套装

sc-523489
200 µg Ab, 40 µg BP
$354.00

MRTF-A/MKL1 (G-8): m-IgG2a BP-HRP 套装

sc-547439
200 µg Ab; 10 µg BP
$354.00

MRTF-A/MKL1 抗体 (G-8) AC

sc-390324 AC
500 µg/ml, 25% agarose
$416.00

MRTF-A/MKL1 抗体 (G-8) HRP

sc-390324 HRP
200 µg/ml
$316.00

MRTF-A/MKL1 抗体 (G-8) FITC

sc-390324 FITC
200 µg/ml
$330.00

MRTF-A/MKL1 抗体 (G-8) PE

sc-390324 PE
200 µg/ml
$343.00

MRTF-A/MKL1 抗体 (G-8) Alexa Fluor® 488

sc-390324 AF488
200 µg/ml
$357.00

MRTF-A/MKL1 抗体 (G-8) Alexa Fluor® 546

sc-390324 AF546
200 µg/ml
$357.00

MRTF-A/MKL1 抗体 (G-8) Alexa Fluor® 594

sc-390324 AF594
200 µg/ml
$357.00

MRTF-A/MKL1 抗体 (G-8) Alexa Fluor® 647

sc-390324 AF647
200 µg/ml
$357.00

MRTF-A/MKL1 抗体 (G-8) Alexa Fluor® 680

sc-390324 AF680
200 µg/ml
$357.00

MRTF-A/MKL1 抗体 (G-8) Alexa Fluor® 790

sc-390324 AF790
200 µg/ml
$357.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-09
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_390324, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 102ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from the antobody work very wellThe antibody works very well and I recommend it for western blot
Date published: 2021-11-07
Rated 3 out of 5 by from The Ab worked in WesternThe Ab detected the MRTF-A in the Western. However, it only worked at 1:200 or lower dilution, which makes it too expensive to use.
Date published: 2018-10-03
Rated 5 out of 5 by from Strong WB dataVery nice western blot results; strong specific signal
Date published: 2017-01-27
Rated 5 out of 5 by from Reliable Antibody, Great for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-16
Rated 5 out of 5 by from RECOMMENDED FOR IF STAININGS The antibodyRECOMMENDED FOR IF STAININGS The antibody gives reliable IF stainings on HeLa and NMuMG cells: excluded (NMuMG)/evenly distributed (HeLa) upon serum starvation, clearly nuclear upon serum stimulation, clearly nuclear upon cytochalasinD treatment, excluded upon latrunculinA treatment. Fixation: 4% PFA 10' at RT. Primary antibody incubation 1:200 in 2% goat serum PBST 0,1% ON at 4 C. We tested sc32909, sc21558 and sc47282 and NONE gave any specific staining (i.e. none recognized a protein that shuttled between the nucleus and the cytoplasm according to what expected in the literature and observed with the sc390324)
Date published: 2015-08-25
Rated 5 out of 5 by from Good Western Blot data of MRTFGood Western Blot data of MRTF-A expression in NIH/3T3 and A549 nuclear extracts and HeLa and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2013-07-02
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_390324, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT
MRTF-A/MKL1 抗体 (G-8) is rated 4.7 out of 5 by 6.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_390324, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 107ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。