021-6093-6350
MRTF-A 抗体 (G-7): sc-398675. Hep G2 (A), HEK293 (B), HUV-EC-C (C) 和 CCRF-CEM (D) 全细胞裂解液及 HeLa 核提取物 (E) 中 MRTF-A 表达的 Western 印迹分析.
MRTF-A/MKL1 抗体 (G-7): sc-398675
4.0(10)
寫評論提問- MRTF-A/MKL1 抗体 G-7 是小鼠单克隆 IgM κ，MRTF-A/MKL1抗体, 在18篇文献中引用，规格为200 µg/ml
- 特异性抗原位于human物种的MRTF-A的C-terminus的氨基酸905-931之间
- 推荐用于 mouse, rat 和 human 来源的MRTF-A WB, IP, IF 和 ELISA检测
- 关于如何获取MRTF-A/MKL1 (G-7): sc-398675的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- 目前，我们还没有完成MRTF-A/MKL1 抗体 (G-7)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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描述
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此外 MRTF 抗体包括 MRTF-A
Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional
MRTF-A抗体（G-7）是一种IgM κ型小鼠单克隆MRTF-A抗体（也称为MKL1抗体、MRTFA抗体或心肌相关转录因子A抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人的MRTF-A蛋白。MRTF-A抗体（G-7）既有非偶联的抗MRTF-A抗体形式，也有多种Alexa Fluor®偶联物形式。血清反应因子（SRF）是一种转录因子，它与血清反应元件（SRE）结合，SRE是一种介导许多细胞基因对生长刺激的瞬时反应的序列。SRF调节几种肌肉基因对生长因子的瞬时反应，并募集辅助成肌因子以激活这些肌肉基因。SRF对于脊椎动物中胚层的形成至关重要，中胚层是心血管系统的起源。心肌素与心肌细胞中的SRF结合，激活心肌启动子。心肌素相关转录因子A（MRTF-A），也称为MKL1，与SRF相互作用并刺激其转录活性。
仅限研究使用。不适用于诊断和治疗用途。
Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标
| 种属 | 基因名称 | 基因ID | 染色体位置 | 亚型（mRNA）登录号 | 蛋白质登录号 | OMIM™ 号码 |
|---|---|---|---|---|---|---|
| Human | MKL1 | Entrez Gene ID: 57591 | 22q13.1 | XM_005261692, XM_005261694, XM_011530283, XM_011530285, XM_011530287, XM_017028888, XM_017028889, NM_001318139, NM_001282660, NM_001282661, NM_001282662, NM_020831 | UniProt ID: Q969V6 | 606078 |
| Mouse | Mkl1 | Entrez Gene ID: 223701 | 15 E1 | XM_006520812, NM_001082536, NM_153049 | UniProt ID: Q8K4J6 | N/A |
针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz®偶联抗体，从而可以省去使用二抗。
圣克鲁斯生物技术的抗体有超过36万的研究引文。抗MRTF-A/MKL1抗体(G-7)在各种科学出版物中有18篇引文。
针对哺乳动物靶标蛋白的一抗，如抗MRTF-A/MKL1 抗体 (G-7)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。 许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。
MRTF-A/MKL1 抗体 (G-7) 规格
替代产品名称: MKL1
产品克隆名称: G-7
分类: 一抗
Clonality: 单克隆抗体
抗体同型: IgM kappa light chain
氨基酸: 905-931
抗原表位靶标: MRTF-A
抗原表位种属: human
产品反应性/检测: MRTF-A
种属反应性/检测: mouse, rat, human
其它哺乳动物反应性: N/A
其它非哺乳动物反应性: N/A
应用: WB, IP, IF, ELISA
瓶的信息: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin
研究引文: 18篇各种科学出版物的引文
MRTF-A/MKL1 抗体 (G-7)可选的偶联物/形式: P: Blocking Peptide
针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.
圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗MRTF-A/MKL1抗体(G-7)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), ELISA (Enzyme-Linked Immunosorbent Assay).
我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。
组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。
单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。
偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。
正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。
優選的 MRTF-A/MKL1 抗體是 MRTF-A/MKL1 抗體 (G-8): sc-390324.
订购信息
| 产品名称 | 产品编号 | 规格 | 价格 | 数量 | 收藏夹 | |
MRTF-A/MKL1 抗体 (G-7) | sc-398675 | 200 µg/ml | $316.00 | |||
MRTF-A/MKL1 (G-7) 中和勝肽 | sc-398675 P | 100 µg/0.5 ml | $68.00 |
Do you recommend G-7 or G-8 for IHC from 4% PFA fixed paraffin embedded tissue on 5 micron sections?
Asked by: MoneyShot
Thank you for your question. I would suggest sc-398675 for IHC use, although either one should be perfectly fine.
Answered by: Tech Service
Date published: 2019-10-18
What secondary antibody do you suggest to use? Your data sheet says it's IgM light chain specific, all mouse IgM HRP I could find are specific for the heavy chain. However your data sheet recommends mouse IgG light chain specific HRP--very confusing......
Asked by: LiUTMDACC
Thank you for your question. We recommend using our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP), m-IgGκ BP-HRP: sc-516102 (see link below). We do not offer a secondary binding protein that is specific to mouse IgM. However, our in-house data indicates that this anti-mouse IgG kappa light chain binding protein will also bind to the kappa light chain of mouse IgM. You could also use any conventional anti-mouse IgM secondary antibody. We do not offer any anti-mouse IgM that is specific for the heavy chain, so I'm not sure what you are referring to, unless those are provided by other suppliers.
https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp
Answered by: Technical Support
Date published: 2017-06-22
Can you recommend a positive control to use with MRTF-A (G-7): sc-398675 monoclonal antibody for Western blot?
Asked by: jenni
We recommend Hep G2 (sc-2227), HEK293 (sc-45136), HUV-EC-C (sc-364180) or CCRF-CEM (sc-2225) whole cell lysates or HeLa nuclear extract (sc-2120) to use as positive controls as we have Western blot data showing specific detection of MRTF-A in these cell lines.
Answered by: Technical Support 3
Date published: 2017-01-17
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Rated 5 out of
5 by
sm26 from
Detection of endogenous MRTF-A/MKL1used for detection of endogenous MRTF-A/MKL1 by immunoblotting
Date published: 2022-05-25
Rated 5 out of
5 by
Kira from
Great fzqtureI bought this one month ago and it is the Best thing!
Date published: 2021-06-01
Rated 4 out of
5 by
yeyan from
MRTF-A抗体评论我一个月前买了该抗体，效果还不错，虽然条带不单一，有很多杂带，但是目的蛋白条带还是很亮，没有其他问题
Date published: 2017-11-07
Rated 5 out of
5 by
Qiao Lyle from
不错申请到了公司的小样，很好用，按照常规RIPA裂解，上样，以前得到的条带都是多条，换抗体后得到了单一的条带。
Date published: 2017-08-27
Rated 5 out of
5 by
ruhulmb from
This antibody works great for meI used this antibody for the detection of MRTF-A protein in 4T1 mouse mamary tumor cell nuclear lysate
Date published: 2017-05-22
Rated 3 out of
5 by
Kauto_lives from
Unsure of specificityIt stains in the correct cellular area but its cellular localization is unspecific. I stained a fibrotic Human and Mouse Skin, liver and Kidney tissue. Staining is specific. However, I did not do an in vitro actin assay to truly determine antibody specificity. MRTF-A is published to be susceptible to actin destabilizing drugs.
Date published: 2017-04-22
Rated 1 out of
5 by
tn310 from
Theona NatisviliThe antibody does not work for ChIP, did not give any signal.
Date published: 2017-04-07
Rated 5 out of
5 by
Dr Ninau Qelp from
Clean bands for WBMRTF-A (G-7) gives nice clean bands for Western blot at 160 kDa. Recommend NIH/3T3 Whole Cell Lysate: sc-2210 as a positive control.
Date published: 2017-01-13
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MRTF-A/MKL1 抗体 (G-7) is rated
4.0 out of
5 by
10.
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版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。