MRCKγ CRISPR/Cas9 KO Plasmid (h): sc-406697
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MRCKγ CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MRCKγ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MRCKγ Antibody (2C3): sc-517148
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRCKγ CRISPR/Cas9 KO Plasmid (h) | sc-406697 | 20 µg | $397.00 |
Overview
CDC42BPG encodes myotonic dystrophy kinase-related Cdc42-binding kinase gamma (MRCKγ), a serine/threonine kinase that functions downstream of the Rho GTPase CDC42 to coordinate actin–myosin contractility. MRCKγ phosphorylates cytoskeletal regulators such as myosin light chain and related substrates to modulate cortical tension, cell polarity, and directional migration. Through these activities it contributes to Rho/CDC42-dependent signaling networks that shape adhesion dynamics, morphogenesis, and invasive behavior. Dysregulation of MRCK family signaling has been linked to altered cytoskeletal remodeling in contexts relevant to tumor cell motility and metastatic dissemination, as well as broader disorders of cell architecture.
MRCKγ CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CDC42BPG gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CDC42BPG together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CDC42BPG open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MRCKγ protein expression.
This CRISPR knockout system enables efficient generation of CDC42BPG-deficient cell models for investigation of MRCKγ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CDC42BPG exon(s) critical for MRCKγ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CDC42BPG genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MRCKγ CRISPR/Cas9 KO Plasmid (h) and MRCKγ CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CDC42BPG locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MRCKγ HDR Plasmid (h) and MRCKγ HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CDC42BPG homology arms to support homology-directed repair at defined CDC42BPG target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.