MPHOSPH6抗体 (D-3) 推荐用于 mouse, rat 和 human 来源的MPHOSPH6 WB, IP, IF, IHC(P) 和 ELISA检测

MPHOSPH6 抗体 (D-3): sc-393429

MPHOSPH6抗体 (D-3) is rated 4.5 out of 5 by 4.
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说明书

    • 抗-MPHOSPH6 抗体 D-3 是小鼠单克隆 IgG1 (kappa轻链),MPHOSPH6抗体,规格为200 µg/ml
    • 免疫原氨基酸序列39-129位于human物种的MPHOSPH6的内部区域内
    • 抗-MPHOSPH6 抗体 (D-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 MPHOSPH6
    • 抗-MPHOSPH6 抗体 (D-3) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取MPHOSPH6 (D-3): sc-393429免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Can MPHOSPH6 (D-3): sc-393429 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes, MPHOSPH6 (D-3): sc-393429 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Good for western blot Good for western blot in 293FT cells (dilution: 1:1000)
Date published: 2017-09-10
Rated 4 out of 5 by from Buen resultado Muy contenta con este anticuerpo. Lo he probado para WB (50ug muestra, concentración 1:500, 1% BSA en PBS), con muestras de ratón y funciona perfectamente.
Date published: 2017-08-01
Rated 5 out of 5 by from very nice for Western blot Works well with human samples, and I got a clean band when incubated the membrane with 1:1000 dilution of primary antibody at 4°C overnight.
Date published: 2017-07-25
Rated 5 out of 5 by from Very good Western blot data of MPHOSPH6 expression Very good Western blot data of MPHOSPH6 expression in HeLa, A-431 and KNRK whole cell lysates. -SCBT QC
Date published: 2014-06-02
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