MLN64 Antibody (H-1): sc-166215
- MLN64 Antibody (H-1) is a mouse monoclonal IgG1 κ MLN64 antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 351-385 within an internal region of MLN64 of human origin
- MLN64 Antibody (H-1) is recommended for detection of MLN64 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-MLN64 Antibody (H-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for MLN64 Antibody (H-1) for WB applications. This reagent is now offered in a bundle with MLN64 Antibody (H-1) (see ordering information below).
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MLN64 Antibody (H-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects MLN64 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MLN64 (H-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MLN64 protein plays a crucial role in cholesterol transport, particularly in the late endosomal compartment, where MLN64 is involved in the trafficking of cholesterol to cytoplasmic receptor sites. This function is vital as MLN64 helps regulate cholesterol levels within cells, which is essential for maintaining cellular membrane integrity and signaling pathways. Dysregulation of cholesterol transport has been implicated in various diseases, including cancer, where amplification of MLN64 gene has been observed in breast cancer cell lines, leading to overexpression and coamplification with ErbB-2. Immunoblot analysis reveals that MLN64 is expressed in a majority of breast cancer cell lines and tissues, as well as in ovarian carcinoma cell lines, while immunofluorescence microscopy demonstrates MLN64 cytoplasmic localization, particularly in condensation sites and perinuclear regions in breast cancer biopsies. MLN64′s ability to modulate cholesterol levels highlights MLN64′s potential as a therapeutic target in conditions characterized by altered lipid metabolism.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MLN64 Antibody (H-1) References:
- The steroidogenic acute regulatory protein homolog MLN64, a late endosomal cholesterol-binding protein. | Alpy, F., et al. 2001. J Biol Chem. 276: 4261-9. PMID: 11053434
- The cholesterol-regulated StarD4 gene encodes a StAR-related lipid transfer protein with two closely related homologues, StarD5 and StarD6. | Soccio, RE., et al. 2002. Proc Natl Acad Sci U S A. 99: 6943-8. PMID: 12011452
- Metastatic lymph node 64 (MLN64), a gene overexpressed in breast cancers, is regulated by Sp/KLF transcription factors. | Alpy, F., et al. 2003. Oncogene. 22: 3770-80. PMID: 12802284
- Molten globule structure and steroidogenic activity of N-218 MLN64 in human placental mitochondria. | Tuckey, RC., et al. 2004. Endocrinology. 145: 1700-7. PMID: 14715710
- Targeted mutation of the MLN64 START domain causes only modest alterations in cellular sterol metabolism. | Kishida, T., et al. 2004. J Biol Chem. 279: 19276-85. PMID: 14963026
- Effect of increasing the expression of cholesterol transporters (StAR, MLN64, and SCP-2) on bile acid synthesis. | Ren, S., et al. 2004. J Lipid Res. 45: 2123-31. PMID: 15342684
- Identification of four novel human genes amplified and overexpressed in breast carcinoma and localized to the q11-q21.3 region of chromosome 17. | Tomasetto, C., et al. 1995. Genomics. 28: 367-76. PMID: 7490069
- MLN64 exhibits homology with the steroidogenic acute regulatory protein (STAR) and is over-expressed in human breast carcinomas. | Moog-Lutz, C., et al. 1997. Int J Cancer. 71: 183-91. PMID: 9139840
- MLN64 contains a domain with homology to the steroidogenic acute regulatory protein (StAR) that stimulates steroidogenesis. | Watari, H., et al. 1997. Proc Natl Acad Sci U S A. 94: 8462-7. PMID: 9237999
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MLN64 Antibody (H-1) | sc-166215 | 200 µg/ml | $322.00 | |||
MLN64 Antibody (H-1): m-IgG1 BP-HRP Bundle | sc-531678 | 200 µg Ab; 20 µg BP | $361.00 | |||
MLN64 Antibody (H-1) AC | sc-166215 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MLN64 Antibody (H-1) HRP | sc-166215 HRP | 200 µg/ml | $322.00 | |||
MLN64 Antibody (H-1) FITC | sc-166215 FITC | 200 µg/ml | $336.00 | |||
MLN64 Antibody (H-1) PE | sc-166215 PE | 200 µg/ml | $349.00 | |||
MLN64 Antibody (H-1) Alexa Fluor® 488 | sc-166215 AF488 | 200 µg/ml | $364.00 | |||
MLN64 Antibody (H-1) Alexa Fluor® 546 | sc-166215 AF546 | 200 µg/ml | $364.00 | |||
MLN64 Antibody (H-1) Alexa Fluor® 594 | sc-166215 AF594 | 200 µg/ml | $364.00 | |||
MLN64 Antibody (H-1) Alexa Fluor® 647 | sc-166215 AF647 | 200 µg/ml | $364.00 | |||
MLN64 Antibody (H-1) Alexa Fluor® 680 | sc-166215 AF680 | 200 µg/ml | $364.00 | |||
MLN64 Antibody (H-1) Alexa Fluor® 790 | sc-166215 AF790 | 200 µg/ml | $364.00 | |||
MLN64 (H-1) Neutralizing Peptide | sc-166215 P | 100 µg/0.5 ml | $69.00 |