Mel-CAM Antibody (1): sc-135987

Mel-CAM Antibody (1) is a mouse monoclonal IgG1 antibody that detects Mel-CAM in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Mel-CAM, also known as MCAM, MUC18, or CD146, is a transmembrane glycoprotein encoded by the human Mel-CAM gene, which plays a crucial role in cell adhesion and is a member of the immunoglobulin superfamily. This protein consists of 603 amino acids, featuring a signal peptide, five immunoglobulin-like domains, a transmembrane region, and a short cytoplasmic tail, which are essential for its function. Mel-CAM is predominantly expressed in advanced primary and metastatic melanomas, as well as in neuroectodermal lineage cell lines, but is absent in normal melanocytes. Notably, Mel-CAM is present in approximately 80% of advanced primary human melanomas and is closely associated with the progression of metastatic disease. The activation of Mel-CAM triggers an outside-in signaling pathway involving protein tyrosine kinases such as Fyn, FAK, and paxillin, which are vital for the dynamics of actin cytoskeleton rearrangement. This process is not only important for tumor cell migration and invasion but also plays a significant role in maintaining thymic architecture and function, highlighting Mel-CAM′s importance in both cancer biology and immune system regulation. Anti-Mel-CAM antibody (1) is an invaluable tool for researchers studying melanoma progression and the underlying mechanisms of cell adhesion and signaling.

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Mel-CAM Antibody (1) References:

1. Laminin-411 is a vascular ligand for MCAM and facilitates TH17 cell entry into the CNS.  |  Flanagan, K., et al. 2012. PLoS One. 7: e40443. PMID: 22792325
2. CD166 positively regulates MCAM via inhibition to ubiquitin E3 ligases Smurf1 and βTrCP through PI3K/AKT and c-Raf/MEK/ERK signaling in Bel-7402 hepatocellular carcinoma cells.  |  Tang, X., et al. 2015. Cell Signal. 27: 1694-702. PMID: 26004137
3. The histone demethylase KDM3A, and its downstream target MCAM, promote Ewing Sarcoma cell migration and metastasis.  |  Sechler, M., et al. 2017. Oncogene. 36: 4150-4160. PMID: 28319067
4. Physical Binding of Endothelial MCAM and Neural Transmembrane Protease Matriptase-Novel Cell Adhesion in Neural Stem cell Vascular Niche.  |  Tung, HH. and Lee, SL. 2017. Sci Rep. 7: 4946. PMID: 28694515
5. Monocyte-released HERV-K dUTPase engages TLR4 and MCAM causing endothelial mesenchymal transition.  |  Otsuki, S., et al. 2021. JCI Insight. 6: PMID: 34185707
6. Phenotype and pathological significance of MCAM+ (CD146+) T cell subset in psoriatic arthritis.  |  Raychaudhuri, SK., et al. 2021. Mol Biol Rep. 48: 6787-6796. PMID: 34491483
7. MCAM is associated with metastasis and poor prognosis in osteosarcoma by modulating tumor cell migration.  |  Du, X., et al. 2022. J Clin Lab Anal. 36: e24214. PMID: 34961985
8. Mcam stabilizes a luminal progenitor-like breast cancer cell state via Ck2 control and Src/Akt/Stat3 attenuation.  |  Balcioglu, O., et al. 2024. NPJ Breast Cancer. 10: 80. PMID: 39277578
9. Expression of Mel-CAM in implantation site intermediate trophoblastic cell line, IST-1, limits its migration on uterine smooth muscle cells.  |  Shih, I., et al. 1998. J Cell Sci. 111 (Pt 17): 2655-64. PMID: 9701564
10. Diagnostic and biological implications of mel-CAM expression in mesenchymal neoplasms.  |  Shih, IM., et al. 1996. Clin Cancer Res. 2: 569-75. PMID: 9816205

The preferred Mel-CAM antibody is Mel-CAM Antibody (P1H12): sc-18837.