Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
MEK kinase-1 Antibody (F-11) - Western Blotting - Image 12569
MEK 激酶-1 抗体 (F-11): sc-17820. A-431 (A), HeLa (B), HL-60 (C), A-10 (D) 和 MDCK (E) 全细胞裂解液中裂解的 MEK 激酶-1 表达的 Western 印迹分析.
MEK 激酶-1 抗体 (F-11): sc-17820. A-431 (A), HeLa (B), HL-60 (C), A-10 (D) 和 MDCK (E) 全细胞裂解液中裂解的 MEK 激酶-1 表达的 Western 印迹分析.

MEK kinase-1 抗体 (F-11): sc-17820

4.5(8)
寫評論提問

说明书
  • MEK kinase-1抗体(F-11)是小鼠单克隆IgG2a κ, 在4篇文献中引用，规格为200 µg/ml
  • 抗-MEK kinase-1 抗体 (F-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 MEK kinase-1; 也和以下物种反应，包括： canine
  • 抗MEK kinase-1抗体（F-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MEK kinase-1 (F-11): sc-17820的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 MEK kinase-1 抗体 (F-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MEK kinase-1 抗体 (F-11) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 MEK kinase 抗体 包括 MEK kinase-1, MEK kinase-2, MEK kinase-3和MEK kinase-4

MEK激酶-1抗体（F-11）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类的MEK激酶-1。MEK激酶-1（F-11）抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。MEK激酶-1在细胞生长因子和其他细胞外信号的细胞反应中起着至关重要的作用。MEK激酶-1负责磷酸化并激活MAP2K1和MAP2K4，从而激活细胞外信号调节激酶（ERK）和c-Jun NH（2）-末端激酶（JNK）通路。这种激活作用可调节多种细胞过程，包括增殖、分化及存活。MEK激酶-1参与NFκB信号通路，激活中央蛋白激酶（如CHUK和IKBKB），从而影响炎症反应和细胞存活。MEK激酶-1通过其PHD结构域利用E3连接酶，通过泛素化降解靶向蛋白，这凸显了MEK激酶-1在维持细胞稳态和调节细胞内蛋白水平方面的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MEK kinase-1 抗体 (F-11) 参考文献:

  1. 一种新型的Jun N-末端激酶（JNK）结合蛋白，它能增强MEK激酶1和TGF-beta激活的激酶1对JNK的激活。  |  Koyano, S., et al. 1999. FEBS Lett. 457: 385-8. PMID: 10471813
  2. c-Abl蛋白酪氨酸激酶响应DNA损伤激活MEK激酶1。  |  Kharbanda, S., et al. 2000. Mol Cell Biol. 20: 4979-89. PMID: 10866655
  3. MEK激酶1与粘着斑激酶相互作用，调节胰岛素受体底物-1的表达。  |  Yujiri, T., et al. 2003. J Biol Chem. 278: 3846-51. PMID: 12458213
  4. MEK激酶1通过NF-κB激活介导Bcr-Abl癌基因的抗凋亡作用。  |  Nawata, R., et al. 2003. Oncogene. 22: 7774-80. PMID: 14586403
  5. MEK激酶1调节角膜形态发生中的c-Jun磷酸化。  |  Zhang, L., et al. 2003. Mol Vis. 9: 584-93. PMID: 14627958
  6. 小鼠胎儿肝脏的最终红细胞生成需要MEK激酶1的活性。  |  Bonnesen, B., et al. 2005. Blood. 106: 3396-404. PMID: 16081685
  7. 在表达活化MEK激酶1的细胞中，低剂量应激刺激可增强细胞凋亡。  |  Widmann, C., et al. 1997. Oncogene. 15: 2439-47. PMID: 9395240
  8. MEK激酶1是DEVD导向的半胱天冬酶的底物，参与基因毒素诱导的细胞凋亡。  |  Widmann, C., et al. 1998. Mol Cell Biol. 18: 2416-29. PMID: 9528810
  9. CD28 信号激活 p21-CDC42/Rac 激活激酶：p21 激活激酶 (PAK) 和 MEK 激酶 1 (MEKK1) 可能介导 CD3 和 CD28 信号之间的相互作用。  |  Kaga, S., et al. 1998. J Immunol. 160: 4182-9. PMID: 9574518
  10. 应激诱导的Fas配体在T细胞中的表达是通过Fas配体启动子中由MEK激酶1调节的反应元件介导的。  |  Faris, M., et al. 1998. Mol Cell Biol. 18: 5414-24. PMID: 9710625

订购信息

产品名称产品编号规格价格数量收藏夹

MEK kinase-1 抗体 (F-11)

sc-17820
200 µg/ml
$316.00

MEK kinase-1 (F-11): m-IgG Fc BP-HRP 套装

sc-525489
200 µg Ab; 10 µg BP
$354.00

MEK kinase-1 (F-11): m-IgG2a BP-HRP 套装

sc-546284
200 µg Ab; 10 µg BP
$354.00

MEK kinase-1 抗体 (F-11) AC

sc-17820 AC
500 µg/ml, 25% agarose
$416.00

MEK kinase-1 抗体 (F-11) HRP

sc-17820 HRP
200 µg/ml
$316.00

MEK kinase-1 抗体 (F-11) FITC

sc-17820 FITC
200 µg/ml
$330.00

MEK kinase-1 抗体 (F-11) PE

sc-17820 PE
200 µg/ml
$343.00

MEK kinase-1 抗体 (F-11) Alexa Fluor® 488

sc-17820 AF488
200 µg/ml
$357.00

MEK kinase-1 抗体 (F-11) Alexa Fluor® 546

sc-17820 AF546
200 µg/ml
$357.00

MEK kinase-1 抗体 (F-11) Alexa Fluor® 594

sc-17820 AF594
200 µg/ml
$357.00

MEK kinase-1 抗体 (F-11) Alexa Fluor® 647

sc-17820 AF647
200 µg/ml
$357.00

MEK kinase-1 抗体 (F-11) Alexa Fluor® 680

sc-17820 AF680
200 µg/ml
$357.00

MEK kinase-1 抗体 (F-11) Alexa Fluor® 790

sc-17820 AF790
200 µg/ml
$357.00

I am using MEK kinase-1 (F-11): sc-17820 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_17820, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 98ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from Didnot work for WBdid not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from good antibody for WBWe get a very good result when dilute with 1:1000. We can see a specific band and the antibody runs smoothly.
Date published: 2017-10-19
Rated 5 out of 5 by from good IHC result.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed using this antibody, and it's very specific and clear.
Date published: 2017-02-24
Rated 5 out of 5 by from Clear stripsPositive signal in WB in mouse brain tissue extract, robust band.
Date published: 2016-12-17
Rated 5 out of 5 by from Excellent antibody for Western blotting.This product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a very potent and highly specific antibody for Western blotting with in diverse human cell lines. It very strongly detects an ~75 kDa protein in immunoblots of a mix of A431, HepG2 and MCF7 cell lysates. This would appear to be a catalytically active fragment of MEKK1.
Date published: 2016-10-13
Rated 5 out of 5 by from Strong bands seen in WB using AStrong bands seen in WB using A-431, HeLa, HL-60, A-10 and MDCK whole cell lysates. -SCBT QC
Date published: 2015-03-13
Rated 5 out of 5 by from WB publishable data with human A549WB publishable data with human A549 - SCBT Publication Review
Date published: 2015-03-11
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic stainingExcellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human colon tissue. -SCBT QC
Date published: 2014-07-05
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_17820, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 21ms
  • REVIEWS, PRODUCT
MEK kinase-1 抗体 (F-11) is rated 4.5 out of 5 by 8.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_17820, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 108ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。