Date published: 2025-9-7
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MEK kinase-1 Antibody (1-9C-2A) - Western Blotting - Image 370524
MEK 激酶-1 抗体 (1-9C-2A): sc-449. c4 (A) 和 NRK (B) 全细胞裂解液中 MEK 激酶-1 表达的 Western 印迹分析.
MEK kinase-1 Antibody (1-9C-2A) - Western Blotting - Image 370524
MEK 激酶-1 抗体 (1-9C-2A): sc-449. c4 (A) 和 NRK (B) 全细胞裂解液中 MEK 激酶-1 表达的 Western 印迹分析.
MEK kinase-1 Antibody (1-9C-2A) - Western Blotting - Image 1846
MEK 激酶-1 抗体 (1-9C-2A): sc-449. A-431 全细胞裂解液中 MEK 激酶-1 表达的 Western 印迹分析. 注意已裂解的 MEK 激酶-1 表达.
MEK 激酶-1 抗体 (1-9C-2A): sc-449. c4 (A) 和 NRK (B) 全细胞裂解液中 MEK 激酶-1 表达的 Western 印迹分析.

MEK kinase-1 抗体 (1-9C-2A): sc-449

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说明书
  • MEK kinase-1 抗体 1-9C-2A 是小鼠单克隆 IgG1 κ，MEK kinase-1抗体, 在17篇文献中引用，规格为200 µg/ml
  • 免疫human来源MEK kinase-1近N-terminus的1-301氨基酸
  • MEK kinase-1 抗体 (1-9C-2A) 推荐用于 WB, IP 和 IF，检测mouse, rat 和human 来源的 MEK kinase-1
  • 抗MEK kinase-1抗体（1-9C-2A）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MEK kinase-1 (1-9C-2A): sc-449的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是MEK kinase-1 Antibody (1-9C-2A) for WB applications. 的首选辅助检测试剂，这些试剂现与MEK kinase-1 Antibody (1-9C-2A) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

额外MEK kinase抗体，包括MEK kinase-1, MEK kinase-2, MEK kinase-3和MEK kinase-4

MEK激酶-1抗体（1-9C-2A）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）检测小鼠、大鼠和人类来源的MEK激酶-1蛋白。MEK激酶-1（1-9C-2A）抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。MEK激酶-1在细胞生长因子和其他细胞外信号的细胞反应中起着至关重要的作用。MEK激酶-1负责磷酸化并激活MAP2K1和MAP2K4，进而激活细胞外信号调节激酶（ERK）和c-Jun NH（2）-末端激酶（JNK）通路。这种激活对于调节细胞的各种过程至关重要，包括增殖、分化以及存活。MEK激酶-1参与NFκB信号通路，激活中央蛋白激酶，如CHUK和IKBKB，这些蛋白激酶对于免疫反应和炎症非常重要。MEK激酶-1通过其PHD结构域利用E3连接酶，通过泛素化靶向蛋白降解，突出了其在维持细胞内稳态和调节细胞内蛋白水平中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MEK kinase-1 抗体 (1-9C-2A) 参考文献:

  1. MEK激酶1（MEKK1）在诱导细胞凋亡方面对微管靶向药物和DNA损伤剂具有不同的作用。  |  Gibson, S., et al. 1999. J Biol Chem. 274: 10916-22. PMID: 10196170
  2. MEK激酶1诱导线粒体通透性转变，导致细胞凋亡，而与细胞色素c释放无关。  |  Gibson, EM., et al. 2002. J Biol Chem. 277: 10573-80. PMID: 11756439
  3. MEK激酶1在TGF-beta/activin诱导的上皮运动和胚胎眼睑闭合中发挥作用。  |  Zhang, L., et al. 2003. EMBO J. 22: 4443-54. PMID: 12941696
  4. 在停止小鼠血流模型中，MEK激酶1的缺失通过抑制平滑肌细胞迁移和尿激酶纤溶酶原激活剂表达来抑制内膜增生。  |  Li, Y., et al. 2005. Circulation. 111: 1672-8. PMID: 15795331
  5. MEK激酶1对于Bcr-Abl诱导的胚胎干细胞中的STAT3和自我更新活性至关重要。  |  Nakamura, Y., et al. 2005. Oncogene. 24: 7592-8. PMID: 16044153
  6. MEK激酶1：小鼠的激酶结构域缺失揭示了其在协调胸腺依赖性免疫和TNFR家族信号传导中的作用。  |  Gallagher, E. 2011. Adv Exp Med Biol. 691: 391-7. PMID: 21153343
  7. 由 MEK 激酶和 Raf 定义的 MAP 激酶调控网络存在分歧。  |  Lange-Carter, CA., et al. 1993. Science. 260: 315-9. PMID: 8385802
  8. 大鼠MEK激酶1 cDNA的克隆揭示了一种内源性膜相关195 kDa蛋白，该蛋白具有较大的调节域。  |  Xu, S., et al. 1996. Proc Natl Acad Sci U S A. 93: 5291-5. PMID: 8643568
  9. 具有与丝裂原活化蛋白激酶同源催化结构域的新型蛋白激酶的分子克隆和表征。  |  Wang, XS., et al. 1996. J Biol Chem. 271: 31607-11. PMID: 8940179
  10. 克隆一种新型丝裂原活化蛋白激酶激酶 MEKK4，它能选择性地调节 c-Jun 氨基末端激酶通路。  |  Gerwins, P., et al. 1997. J Biol Chem. 272: 8288-95. PMID: 9079650
  11. MEK 激酶受 EGF 调节，并选择性地与 Rac/Cdc42 相互作用。  |  Fanger, GR., et al. 1997. EMBO J. 16: 4961-72. PMID: 9305638
  12. Grb2与MEK激酶1的相互作用参与调节表皮生长因子对Jun激酶活性的影响。  |  Pomérance, M., et al. 1998. J Biol Chem. 273: 24301-4. PMID: 9733714

订购信息

产品名称产品编号规格价格数量收藏夹

MEK kinase-1 抗体 (1-9C-2A)

sc-449
200 µg/ml
$316.00

MEK kinase-1 (1-9C-2A): m-IgG Fc BP-HRP 套装

sc-526458
200 µg Ab; 10 µg BP
$354.00

MEK kinase-1 (1-9C-2A): m-IgG1 BP-HRP 套装

sc-531831
200 µg Ab; 20 µg BP
$354.00

MEK kinase-1 抗体 (1-9C-2A) AC

sc-449 AC
500 µg/ml, 25% agarose
$416.00

MEK kinase-1 抗体 (1-9C-2A) HRP

sc-449 HRP
200 µg/ml
$316.00

MEK kinase-1 抗体 (1-9C-2A) FITC

sc-449 FITC
200 µg/ml
$330.00

MEK kinase-1 抗体 (1-9C-2A) PE

sc-449 PE
200 µg/ml
$343.00

MEK kinase-1 抗体 (1-9C-2A) Alexa Fluor® 488

sc-449 AF488
200 µg/ml
$357.00

MEK kinase-1 抗体 (1-9C-2A) Alexa Fluor® 546

sc-449 AF546
200 µg/ml
$357.00

MEK kinase-1 抗体 (1-9C-2A) Alexa Fluor® 594

sc-449 AF594
200 µg/ml
$357.00

MEK kinase-1 抗体 (1-9C-2A) Alexa Fluor® 647

sc-449 AF647
200 µg/ml
$357.00

MEK kinase-1 抗体 (1-9C-2A) Alexa Fluor® 680

sc-449 AF680
200 µg/ml
$357.00

MEK kinase-1 抗体 (1-9C-2A) Alexa Fluor® 790

sc-449 AF790
200 µg/ml
$357.00

Are there any issues with using sc-449: MEK kinase-1 (1-9C-2A) mouse monoclonal primary antibody on mouse samples?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-449 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 1 out of 5 by from Did not workTried this on HeLa lysates (40 ug lysate loaded per lane). I did not see anything for 1:1000, 1:500 or even 1:200 dilution. 1:100 shows multiple bands, but nothing at the predicted MW.
Date published: 2022-02-14
Rated 5 out of 5 by from remarkableI used diluted 1/1000 to get the strong band, which was in the right place for the target band, the band about 80kDa
Date published: 2017-10-18
Rated 5 out of 5 by from obtained expected resultThis antibody against the target protein MEK kinase-1, specific band presen at the target position,while the siRNA interfere controled lane shows weak expression,very specific and satisfied result by diluted the antibody at 1:500.
Date published: 2017-07-29
Rated 5 out of 5 by from western blotThe antibody used for Western blot, BEWO cell lines, Wenstern blot bands were clear, no obvious impurity band, we have another gene, antibody effect is given before the good, we will continue to order the company's antibody products
Date published: 2017-02-20
Rated 5 out of 5 by from Excellent!This antibody is great in western blot analysis of MEK kinase-1 expression in A-431 whole cell lysate.
Date published: 2017-02-03
Rated 5 out of 5 by from See the signalWe experience some troubles at first while trying to determine the right dilution,we ended up seeing signal at dilution 1:200.
Date published: 2016-12-17
Rated 4 out of 5 by from Band seen in WB using ABand seen in WB using A-431 whole cell lysate. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from This antibody was used in a publication forThis antibody was used in a publication for KA and WB on transfetced LnCAP - SCBT Publication Review
Date published: 2015-04-23
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MEK kinase-1 抗体 (1-9C-2A) is rated 4.5 out of 5 by 11.
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