Date published: 2025-9-7

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Max 抗体 (H-2): sc-8011

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说明书
  • Max 抗体 H-2 是小鼠单克隆 IgG1 κ,Max抗体, 在29篇文献中引用,规格为200 µg/ml
  • 免疫human来源Max位于C-terminus 的28-151氨基酸
  • Max 抗体 (H-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Max p21 and Max p22; 也和以下物种反应,包括: canine
  • 抗Max抗体(H-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-8011 X, 200 µg/0.1 ml)
  • 关于如何获取Max (H-2): sc-8011免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Max 抗体 (H-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Max 抗体 (H-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Max抗体(H-2)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类样本中的Max蛋白。Max抗体(H-2)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®选项。Max蛋白在转录因子复合物中作为基因表达的关键调节因子,特别是与Myc蛋白形成异二聚体,以增强DNA结合和转录激活。Max-Myc异二聚体与E-box序列CACGTG的结合强度高于Max同二聚体,从而影响靶基因的表达。Max在细胞中的稳定存在与高度调节的Myc不同,可确保转录网络的正常功能。Max与Mad和Mxi1等蛋白的相互作用可抑制Max-Myc活性,从而凸显细胞行为和肿瘤发生中的复杂调节机制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Max 抗体 (H-2) 参考文献:

  1. 转录因子myc-max/max-max和E-box DNA结合常数的测定:抑制剂对结合的影响。  |  Park, S., et al. 2004. Biochim Biophys Acta. 1670: 217-28. PMID: 14980448
  2. Myc家族的癌蛋白通过共同的途径在培养中转化正常细胞:Max和反式作用显性突变体的交叉干扰。  |  Mukherjee, B., et al. 1992. Genes Dev. 6: 1480-92. PMID: 1644290
  3. Myn(max 的小鼠同源物)与 c-Myc 的结合可刺激甲基化敏感 DNA 结合和 ras 共转化。  |  Prendergast, GC., et al. 1991. Cell. 65: 395-407. PMID: 1840505
  4. 细胞内亮氨酸拉链的相互作用表明 c-Myc 具有异质偶联性。  |  Dang, CV., et al. 1991. Mol Cell Biol. 11: 954-62. PMID: 1990293
  5. Max:一种螺旋-环-螺旋拉链蛋白,与 Myc 形成序列特异的 DNA 结合复合物。  |  Blackwood, EM. and Eisenman, RN. 1991. Science. 251: 1211-7. PMID: 2006410
  6. 小分子调节剂对c-Myc/Max和Max/Max相互作用的影响。  |  Berg, T. 2011. Curr Top Microbiol Immunol. 348: 139-49. PMID: 20680803
  7. 二聚化的转录调控:乱伦关系的两面。  |  Jones, N. 1990. Cell. 61: 9-11. PMID: 2180585
  8. MAX与减数分裂开始之间的意外联系。  |  Okuda, A. and Suzuki, A. 2016. Cell Cycle. 15: 2235-6. PMID: 27267300
  9. c-Myc/Max和Max/Max二聚体对DNA的特异性结合。  |  Solomon, DL., et al. 1993. Nucleic Acids Res. 21: 5372-6. PMID: 8265351
  10. Mad:Max 的异源二聚体伙伴,可颉颃 Myc 的转录活性。  |  Ayer, DE., et al. 1993. Cell. 72: 211-22. PMID: 8425218
  11. Mxi1是一种能与Max发生特异性相互作用的蛋白质,能与Myc-Max识别位点结合。  |  Zervos, AS., et al. 1993. Cell. 72: 223-32. PMID: 8425219
  12. c-Myc 蛋白的致癌活性需要与 Max 进行二聚。  |  Amati, B., et al. 1993. Cell. 72: 233-45. PMID: 8425220

订购信息

产品名称产品编号规格价格数量收藏夹

Max 抗体 (H-2)

sc-8011
200 µg/ml
$316.00

Max (H-2): m-IgG Fc BP-HRP 套装

sc-528200
200 µg Ab; 10 µg BP
$354.00

Max (H-2): m-IgGκ BP-HRP 套装

sc-520522
200 µg Ab, 40 µg BP
$354.00

Max (H-2): m-IgG1 BP-HRP 套装

sc-542812
200 µg Ab; 20 µg BP
$354.00

Max 抗体 (H-2) X

sc-8011 X
200 µg/0.1 ml
$316.00

Max 抗体 (H-2) AC

sc-8011 AC
500 µg/ml, 25% agarose
$416.00

Max 抗体 (H-2) HRP

sc-8011 HRP
200 µg/ml
$316.00

Max 抗体 (H-2) FITC

sc-8011 FITC
200 µg/ml
$330.00

Max 抗体 (H-2) PE

sc-8011 PE
200 µg/ml
$343.00

Max 抗体 (H-2) Alexa Fluor® 488

sc-8011 AF488
200 µg/ml
$357.00

Max 抗体 (H-2) Alexa Fluor® 546

sc-8011 AF546
200 µg/ml
$357.00

Max 抗体 (H-2) Alexa Fluor® 594

sc-8011 AF594
200 µg/ml
$357.00

Max 抗体 (H-2) Alexa Fluor® 647

sc-8011 AF647
200 µg/ml
$357.00

Max 抗体 (H-2) Alexa Fluor® 680

sc-8011 AF680
200 µg/ml
$357.00

Max 抗体 (H-2) Alexa Fluor® 790

sc-8011 AF790
200 µg/ml
$357.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Service 11
Date published: 2017-01-11

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-8011 PE), fluorescein (sc-8011 FITC), Alexa Fluor® 488 (sc-8011 AF488) or Alexa Fluor® 647 (sc-8011 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Service 11
Date published: 2017-01-11
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Rated 1 out of 5 by from Hat leider nicht funktioniert.Verschiedene Verdünnungen (1:1000 bis herunter auf 1:50) unter Verwendung von Standard Immunoblot Methoden und Proteinextrakten aus humanen Zellen führten zu keinem Signal auf Höhe der 22-kDa Bande.
Date published: 2021-07-06
Rated 5 out of 5 by from Check MAX protein expression cancer cellsWestern blot antibody:sc-8011 result:Postive in prostate cancer cell line(LNCaP Cell)
Date published: 2017-10-31
Rated 5 out of 5 by from works wellWorks well in western blot analysis with clean background.
Date published: 2017-10-15
Rated 4 out of 5 by from mouse ES cellsthis max antibody could work in mouse ES cells ,good
Date published: 2017-04-07
Rated 5 out of 5 by from Superb westernVery clear western blot data; strong signal with low background
Date published: 2017-01-29
Rated 5 out of 5 by from Great, versatile antibodyThe Max Antibody (H-2): sc-8011 works very well for a variety of applications including WB, IP, IF, and IHC(P). It has also been cited multiple times for further verification.
Date published: 2017-01-13
Rated 5 out of 5 by from Worked well for western blot with human cellWorked well for western blot with human cell lines: HT29-APC & HT29-beta-galactosidase. -SCBT Publication Review
Date published: 2015-05-08
Rated 5 out of 5 by from EMSA and Western Blot: published results in HeLaEMSA and Western Blot: published results in HeLa, RMS-13, and Tera-1 using H-2 antibody. -SCBT Publication Review
Date published: 2015-04-01
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Max 抗体 (H-2) is rated 4.5 out of 5 by 11.
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