Date published: 2025-9-11

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Mast Cell Chymase 抗体 (CC1): sc-59586

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说明书
  • Mast Cell Chymase 抗体 CC1 是小鼠单克隆 IgG1 κ,Mast Cell Chymase抗体, 在16篇文献中引用,规格为200 µg/ml
  • 针对纯化的human来源皮肤糜蛋白酶
  • Mast Cell Chymase 抗体 (CC1) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 Mast Cell Chymase distributed in skin, synovium, lung and heart; 也和以下物种反应,包括: 和 porcine and canine
  • 抗Mast Cell Chymase抗体(CC1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Mast Cell Chymase (CC1): sc-59586免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成Mast Cell Chymase 抗体 (CC1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

肥大细胞糜蛋白酶抗体(CC1)是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过西部印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和石蜡包埋切片免疫组织化学(IHCP)检测小鼠、大鼠和人源肥大细胞糜蛋白酶蛋白。肥大细胞糜蛋白酶(CC1)抗体有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种 Alexa Fluor® 共轭物。肥大细胞糜蛋白酶又称 CMA1 或 MCT1,通过产生血管活性肽和降解细胞外基质在免疫反应中发挥着至关重要的作用,而细胞外基质对组织重塑和炎症反应至关重要。肥大细胞糜蛋白酶主要存在于肥大细胞中,肥大细胞是一种特化的免疫细胞,在过敏反应和炎症过程中释放介质。肥大细胞糜蛋白酶存在于这些细胞中,凸显了它在调节局部免疫反应和维持组织平衡方面的重要性。人类糜蛋白酶基因位于染色体 14q11.2 上,编码的前酶包括 19 个氨基酸的信号肽、2 个氨基酸的酸性前肽和 226 个氨基酸的催化结构域,结构复杂,功能重要。肥大细胞糜蛋白酶被归类为糜丝氨酸蛋白酶,属于肽酶家族 S1,这突出了它在处理各种底物时的酶活性。此外,小鼠和大鼠肥大细胞蛋白酶 1 蛋白是人类胰蛋白酶的同源物,在氨基酸水平上有 76% 的序列相同性,因此它们在研究肥大细胞生物学及其在健康和疾病中的作用方面很有价值。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Mast Cell Chymase 抗体 (CC1) 参考文献:

  1. 肥大细胞的多种作用  |  Gurish, MF. and Austen, KF. 2001. J Exp Med. 194: F1-5. PMID: 11435478
  2. 三种小鼠肥大细胞特异性丝氨酸蛋白酶 MMCP-1, MMCP-4 和 MMCP-5 的克隆与结构分析。  |  Huang, RY., et al. 1991. Eur J Immunol. 21: 1611-21. PMID: 2060576
  3. 人类肥大细胞糜蛋白酶基因的结构, 染色体分配和推导的氨基酸序列。  |  Caughey, GH., et al. 1991. J Biol Chem. 266: 12956-63. PMID: 2071582
  4. 人类肥大细胞糜蛋白酶基因(CMA1):与糜蛋白酶 G/酶基因簇的映射关系及系限制性表达。  |  Caughey, GH., et al. 1993. Genomics. 15: 614-20. PMID: 8468056

订购信息

产品名称产品编号规格价格数量收藏夹

Mast Cell Chymase 抗体 (CC1)

sc-59586
200 µg/ml
$316.00

Mast Cell Chymase 抗体 (CC1) AC

sc-59586 AC
500 µg/ml, 25% agarose
$416.00

Mast Cell Chymase 抗体 (CC1) HRP

sc-59586 HRP
200 µg/ml
$316.00

Mast Cell Chymase 抗体 (CC1) FITC

sc-59586 FITC
200 µg/ml
$330.00

Mast Cell Chymase 抗体 (CC1) PE

sc-59586 PE
200 µg/ml
$343.00

Mast Cell Chymase 抗体 (CC1) Alexa Fluor® 488

sc-59586 AF488
200 µg/ml
$357.00

Mast Cell Chymase 抗体 (CC1) Alexa Fluor® 546

sc-59586 AF546
200 µg/ml
$357.00

Mast Cell Chymase 抗体 (CC1) Alexa Fluor® 594

sc-59586 AF594
200 µg/ml
$357.00

Mast Cell Chymase 抗体 (CC1) Alexa Fluor® 647

sc-59586 AF647
200 µg/ml
$357.00

Mast Cell Chymase 抗体 (CC1) Alexa Fluor® 680

sc-59586 AF680
200 µg/ml
$357.00

Mast Cell Chymase 抗体 (CC1) Alexa Fluor® 790

sc-59586 AF790
200 µg/ml
$357.00

What is the optimal concentration for flow testing? Cat #:sc-59586 Batch #:E2318

Asked by: Anonymous
Thank you for your question. We recommend 1 µg normal immunoglobulins per 1 million cells. Here is our recomended protocol:https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2022-09-07

To Technical support I want to use this mast cell chymase (CC1): SC-59586 to detect Chymase in Rat Basophilic Leukemia cells (RBL-2H3). I want to know, Can I use it? Best regards,

Asked by: Pual
Thank you for your question. Yes, this antibody is recommended for use on mouse, rat and human samples, so you will be fine for this use. We do not have any information regarding Mast Cell Chymase expression in your specific cell type, so that we can not guarantee.
Answered by: Tech Service
Date published: 2019-08-21

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 7
Date published: 2017-01-22
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Rated 5 out of 5 by from Very good antibodyClear band observed by WB using human recombinant chymase, no background
Date published: 2017-06-01
Rated 5 out of 5 by from Excellent choice for IHCThe CC1 clone is an excellent monoclonal antibody for IHC detection of Mast Cell Chymase.
Date published: 2016-12-22
Rated 5 out of 5 by from Publishable IHC data with human sinus mucosaPublishable IHC data with human sinus mucosa and NP - SCBT Publication Review
Date published: 2015-05-14
Rated 5 out of 5 by from Good specificity and signal strength in humanGood specificity and signal strength in human skin chymase and human recombinant chymase -SCBT QC
Date published: 2015-01-04
Rated 5 out of 5 by from IHCP publishable data with mouse brainIHCP publishable data with mouse brain - SCBT Publication Review
Date published: 2014-07-10
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Mast Cell Chymase 抗体 (CC1) is rated 5.0 out of 5 by 5.
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