Date published: 2025-9-6

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LC3B 抗体 (G-9): sc-376404

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说明书
  • LC3B抗体(G-9)是小鼠单克隆IgG2b κ, 在91篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的MAP LC3β的N-terminus附近的氨基酸3-39之间
  • LC3B 抗体 (G-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测 MAP LC3β and MAP LC3β2 of human origin and MAP LC3β of mouse and rat origin
  • 抗LC3B抗体(G-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取LC3B (G-9): sc-376404免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 LC3B 抗体 (G-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与LC3B 抗体 (G-9) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

MAP LC3β 抗体(G-9)是一种 IgG2b κ 小鼠单克隆 MAP LC3β 抗体(也称为 MAP LC3β 抗体或 MAP LC3 抗体),它通过 WB、IP、IF、IHC(P) 和 ELISA 方法检测人源的 MAP LC3β 和 MAP LC3β2,以及小鼠和大鼠来源的 MAP LC3β。MAP LC3β 抗体(G-9)既可以是非偶联的抗 MAP LC3β 抗体形式,也可以是多种偶联形式的抗 MAP LC3β 抗体,包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。微管相关蛋白(MAPs)调节微管的稳定性,并在神经元发育以及维持神经元可塑性和刚性之间的平衡中发挥关键作用。MAP-轻链 3 beta(MAP-LC3β)和 MAP-轻链 3 alpha(MAP-LC3α)是 MAP1A 和 MAP1B 的亚基。MAP-LC3β 是 Apg8p 的同源物,对自噬至关重要,并在加工后与自噬体膜相关联。LC3β 的两种形式,即胞质 LC3-I 和膜结合 LC3-II,都是在翻译后产生的。LC3-I 是通过从新合成的 LC3β 中去除 C 末端的 22 个氨基酸而形成的,随后一部分 LC3-I 转化为 LC3-II。LC3 通过与 60S 核糖体结合并结合到纤连蛋白 mRNA 的 3' 非翻译区的 AU 富集元件上,增强动脉导管细胞中纤连蛋白 mRNA 的翻译。这有助于将纤连蛋白 mRNA 分选到粗糙内质网上并进行翻译。MAP LC3β 也可能参与自噬体空泡的形成。它主要在心脏、睾丸、大脑和骨骼肌中表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

LC3B 抗体 (G-9)

sc-376404
200 µg/ml
$316.00

LC3B (G-9): m-IgGκ BP-HRP 套装

sc-522943
200 µg Ab, 40 µg BP
$354.00

LC3B (G-9): m-IgG2b BP-HRP 套装

sc-549081
200 µg Ab; 10 µg BP
$354.00

LC3B 抗体 (G-9) AC

sc-376404 AC
500 µg/ml, 25% agarose
$416.00

LC3B 抗体 (G-9) HRP

sc-376404 HRP
200 µg/ml
$316.00

LC3B 抗体 (G-9) FITC

sc-376404 FITC
200 µg/ml
$330.00

LC3B 抗体 (G-9) PE

sc-376404 PE
200 µg/ml
$343.00

LC3B 抗体 (G-9) Alexa Fluor® 488

sc-376404 AF488
200 µg/ml
$357.00

LC3B 抗体 (G-9) Alexa Fluor® 546

sc-376404 AF546
200 µg/ml
$357.00

LC3B 抗体 (G-9) Alexa Fluor® 594

sc-376404 AF594
200 µg/ml
$357.00

LC3B 抗体 (G-9) Alexa Fluor® 647

sc-376404 AF647
200 µg/ml
$357.00

LC3B 抗体 (G-9) Alexa Fluor® 680

sc-376404 AF680
200 µg/ml
$357.00

LC3B 抗体 (G-9) Alexa Fluor® 790

sc-376404 AF790
200 µg/ml
$357.00

LC3B (G-9) 中和勝肽

sc-376404 P
100 µg/0.5 ml
$68.00

I would like to receive a FREE 10 µg sample of LC3B (G-9): sc-376404

Asked by: Carla Pernaci
Thank you for contacting Santa Cuz Biotechnology. Please email technical service for details on our sample program scbt@scbt.com
Answered by: BlakeJ
Date published: 2024-03-28

what host is the antibody from? is it mouse? If so, how can we use this antibody to do IF?

Asked by: ruicong
Thank you for your question. MAP LC3β Antibody (G-9) is a mouse monoclonal IgG2b and is recommended for IF applications based on this protocol: https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: Tech Support Europe
Date published: 2020-12-28

Can you recommend a negative control to use with MAP LC3β Antibody (G-9): sc-376404?

Asked by: jenni
We recommend using the blocking peptide sc-376404 P as a negative control. Antibody binding to antigen may be blocked/competed by pre-absorption with the blocking peptide. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 12
Date published: 2017-01-09
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Rated 4 out of 5 by from Funziona bene anche su cellule canineAb che funziona bene anche su cellule muscolari di cane
Date published: 2025-04-03
Rated 5 out of 5 by from Excellent in western blottingWestern Blotting Dilution 1:1000 MDA cell lysate Stong and specific signals (two band at the expected molecular weight) that respond to cell starvation (lower band increases)
Date published: 2020-06-11
Rated 3 out of 5 by from okLC3BII strong, but LC3BI weak. Difficult to calculate ratio LC3BII:LC3BI
Date published: 2020-02-06
Rated 4 out of 5 by from Good for immunostainingI have been using this antibody for immunocytochemistry and got good staining. for western bloting have difficulty to see both band
Date published: 2019-11-26
Rated 4 out of 5 by from Good Ab for IFThis antibody is good for immunofluorescent staining (1:100 in 1%BSA in PBS). Extensive washes are necessary!
Date published: 2018-11-13
Rated 5 out of 5 by from Good for western blotI tried this ab for western blot using HEK 293 cells. As shown, 5ug cell lysates yielded quite clean results with1:500 dilution.
Date published: 2018-10-03
Rated 5 out of 5 by from Good antibodyWorks well in mouse cell lysates. Loaded 30 ug of protein. Blocked in 5% milk. Overnight incubation with primary antibody (1:200). Got two distinct bands around 20 kDa.
Date published: 2018-08-15
Rated 4 out of 5 by from Works well on ICC of rat primary neuronsWe received this antibody as a sample to try for ICC. We used the antibody at 1:50 and the secondary antibody was donkey anti-mouse Alexa 488 at 1:1000. It worked well but overall the G-2 antibody (sc271625) seemed to work a little better for this application.
Date published: 2018-04-11
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LC3B 抗体 (G-9) is rated 4.7 out of 5 by 20.
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