Date published: 2025-9-6

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LC3A/LC3B 抗体 (G-4): sc-398822

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说明书
  • LC3A/LC3B 抗体 G-4 是小鼠单克隆 IgG1 κ,LC3A/LC3B抗体, 在101篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的MAP LC3β的内部区域内的氨基酸33-56之间
  • LC3A/LC3B 抗体 (G-4) 推荐用于 WB, IP, IF 和 ELISA,检测 MAP LC3α and MAP LC3β of mouse, rat and human origin and MAP LC3β2 of human origin
  • 抗LC3A/LC3B抗体(G-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取LC3A/LC3B (G-4): sc-398822免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是LC3A/LC3B Antibody (G-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与LC3A/LC3B Antibody (G-4) 打包提供(请参阅下面的订购信息)。

快捷链接

関連項目

MAP LC3α/β抗体(G-4)是一种IgG1κ小鼠单克隆MAP LC3α/β抗体(也被称为MAP LC3抗体、MAP1LC3A抗体、MAP1LC3B抗体或微管相关蛋白1轻链3αβ抗体),通过WB、IP、IF和ELISA检测小鼠、大鼠和人的MAP LC3α和MAP LC3β以及人的MAP LC3β2。MAP LC3α/β 抗体(G-4)既可以是非偶联的抗 MAP LC3 α/β 抗体形式,也可以是多种偶联形式的抗 MAP LC3 α/β 抗体,包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。微管相关蛋白(MAPs)可调节微管的稳定性,并在神经元发育以及维持神经元可塑性与刚性之间的平衡中发挥关键作用。MAP-轻链 3 β(MAP-LC3β)和 MAP-轻链 3 α(MAP-LC3α)是可以与 MAP-1A 或 MAP-1B 结合的亚基。MAP-LC3β 对于自噬至关重要,并且在加工后与自噬体膜相关,而 MAP LC3α 则参与自噬体空泡的形成,并定位于胞质膜。MAP LC3α 表达为两种选择性剪接的异构体,它们在睾丸、大脑、心脏、肝脏和骨骼肌中表达,但在胸腺和外周血白细胞中缺失。MAP LC3β 以胞质和膜结合形式存在,也可能参与自噬体空泡的形成,并且主要在心脏、睾丸、大脑和骨骼肌中表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

LC3A/LC3B 抗体 (G-4) 参考文献:

  1. 膜转运调节器和哺乳动物自噬吞噬因子 Aut7p 的直系同源物 GATE-16 的结构。  |  Paz, Y., et al. 2000. J Biol Chem. 275: 25445-50. PMID: 10856287
  2. LC3是酵母Apg8p在哺乳动物中的同源物,在加工后定位在自噬体膜上。  |  Kabeya, Y., et al. 2000. EMBO J. 19: 5720-8. PMID: 11060023
  3. 酿酒酵母 Apg7p 的人类同源物是一种蛋白激活酶,可激活多种底物,包括人类 Apg12p, GATE-16, GABARAP 和 MAP-LC3。  |  Tanida, I., et al. 2001. J Biol Chem. 276: 1701-6. PMID: 11096062
  4. 人类微管相关蛋白 1a(MAP1A)基因:基因组组织, cDNA 序列以及发育和组织特异性表达。  |  Fink, JK., et al. 1996. Genomics. 35: 577-85. PMID: 8812494
  5. 轻链 3 的基因定位和发育表达:微管相关蛋白 1A(MAP1A)和 MAP1B 的共同亚基  |  Mann, SS. and Hammarback, JA. 1996. J Neurosci Res. 43: 535-44. PMID: 8833088
  6. 微管相关蛋白 1 轻链 3 是一种纤维粘连蛋白 mRNA 结合蛋白,与羔羊血管平滑肌细胞的 mRNA 翻译有关。  |  Zhou, B., et al. 1997. J Clin Invest. 100: 3070-82. PMID: 9399954
  7. 微管参与血管平滑肌细胞中微管相关蛋白 1 的轻链 3 对纤维连接蛋白表达的翻译调控。  |  Zhou, B. and Rabinovitch, M. 1998. Circ Res. 83: 481-9. PMID: 9734470

订购信息

产品名称产品编号规格价格数量收藏夹

LC3A/LC3B 抗体 (G-4)

sc-398822
200 µg/ml
$316.00

LC3A/LC3B (G-4): m-IgG Fc BP-HRP 套装

sc-530941
200 µg Ab; 10 µg BP
$354.00

LC3A/LC3B (G-4): m-IgGκ BP-HRP 套装

sc-524501
200 µg Ab, 40 µg BP
$354.00

LC3A/LC3B (G-4): m-IgG1 BP-HRP 套装

sc-544327
200 µg Ab; 20 µg BP
$354.00

LC3A/LC3B 抗体 (G-4) AC

sc-398822 AC
500 µg/ml, 25% agarose
$416.00

LC3A/LC3B 抗体 (G-4) HRP

sc-398822 HRP
200 µg/ml
$316.00

LC3A/LC3B 抗体 (G-4) FITC

sc-398822 FITC
200 µg/ml
$330.00

LC3A/LC3B 抗体 (G-4) PE

sc-398822 PE
200 µg/ml
$343.00

LC3A/LC3B 抗体 (G-4) Alexa Fluor® 488

sc-398822 AF488
200 µg/ml
$357.00

LC3A/LC3B 抗体 (G-4) Alexa Fluor® 546

sc-398822 AF546
200 µg/ml
$357.00

LC3A/LC3B 抗体 (G-4) Alexa Fluor® 594

sc-398822 AF594
200 µg/ml
$357.00

LC3A/LC3B 抗体 (G-4) Alexa Fluor® 647

sc-398822 AF647
200 µg/ml
$357.00

LC3A/LC3B 抗体 (G-4) Alexa Fluor® 680

sc-398822 AF680
200 µg/ml
$357.00

LC3A/LC3B 抗体 (G-4) Alexa Fluor® 790

sc-398822 AF790
200 µg/ml
$357.00

LC3A/LC3B (G-4) 中和勝肽

sc-398822 P
100 µg/0.5 ml
$68.00

What kind of secondary detection reagent works best with this mouse monoclonal primary antibody?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-09

Due to some issues, I will not be able to do methanol fixation for IF and have to stick with 4% PFA followed by TritonX-100 permeabilization. Will this antibody work in those conditions?

Asked by: Anjali
Thank you for your question. This antibody, MAP LC3α/β (G-4): sc-398822, has not yet been tested on formalin or PFA fixed tissues or cells so we do not know if it will work in this application. However, we would recommend including a permeabilization step, such as with Triton X-100, if using 4% PFA as a fixative.
Answered by: Technical Support
Date published: 2016-12-20

What fixation method do you recommend for IF (human fibroblasts)?

Asked by: Steven94
Thank you for your question. Our recommended fixation method for tissue culture cells is 5 minutes in -10° C methanol. Here is a link to our complete recommended protocol for immunofluorescence cell staining. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2016-11-14
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Rated 5 out of 5 by from Works well for WBWorks well on neonatal mouse lungs at concentration of 30ug. Antibody dilution will be 1:250 for overnight at 4oC
Date published: 2024-12-13
Rated 2 out of 5 by from Specificity is poor?Wrong band?Hi, We tried this antibody five times for mouse solid tumor. But It signals in the range of 25-34 kDA. We diluted it with 3% milk powder at 1/500 and 1/1000 ratios. And each time we capture the signal in the range of 55-72 kDa. Where are we going wrong?
Date published: 2022-10-03
Rated 3 out of 5 by from Specificity is poor?For IF,The signal is present in the nucleus and cytoplasm.
Date published: 2022-08-31
Rated 3 out of 5 by from Not badWe used this antibody to assess protein levels in human non-small cell lung cancer cells by western blot. We got a good signal with 1:500 dilution.
Date published: 2022-01-21
Rated 5 out of 5 by from It works very well!I used it in total lysates diluted at 1:1000. It recognizes a strong band.
Date published: 2021-12-02
Rated 4 out of 5 by from it works goodSC398822 it works at first try using 20ug mouse brain lysate. 1:1000 dilution is good.
Date published: 2020-07-02
Rated 4 out of 5 by from Buena señal.Detectamos solo la banda de LC3a; células estrelladas.
Date published: 2020-05-09
Rated 2 out of 5 by from FairLC3BII strong, but LC3BI weak. Difficult to calculate ratio LC3BII:LC3BI
Date published: 2020-03-04
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LC3A/LC3B 抗体 (G-4) is rated 4.2 out of 5 by 49.
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