MAP LC3 alpha / beta抗体 (G-4) 推荐用于 MAP LC3α and MAP LC3β of mouse, rat and human origin and MAP LC3β2 of human origin WB, IP, IF 和 ELISA检测

MAP LC3α/β 抗体 (G-4): sc-398822

MAP LC3α/β抗体 (G-4) is rated 4.2 out of 5 by 44.
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说明书

    • 抗-MAP LC3 alpha / beta 抗体 G-4 是小鼠单克隆 IgG1 κ,MAP LC3α/β抗体, 在37篇文献中引用,规格为200 µg/ml
    • 特异性抗原位于human物种的MAP LC3β的内部区域内的氨基酸33-56之间
    • MAP LC3 alpha / beta 抗体 (G-4) 推荐用于 WB, IP, IF 和 ELISA,检测 MAP LC3α and MAP LC3β of mouse, rat and human origin and MAP LC3β2 of human origin
    • 抗-MAP LC3 alpha / beta 抗体 (G-4) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取MAP LC3α/β (G-4): sc-398822免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for MAP LC3α/β Antibody (G-4) for WB applications. These reagents are now offered in bundles with MAP LC3α/β Antibody (G-4) (see ordering information below).

What kind of secondary detection reagent works best with this mouse monoclonal primary antibody?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-09

Due to some issues, I will not be able to do methanol fixation for IF and have to stick with 4% PFA followed by TritonX-100 permeabilization. Will this antibody work in those conditions?

Asked by: Anjali
Thank you for your question. This antibody, MAP LC3α/β (G-4): sc-398822, has not yet been tested on formalin or PFA fixed tissues or cells so we do not know if it will work in this application. However, we would recommend including a permeabilization step, such as with Triton X-100, if using 4% PFA as a fixative.
Answered by: Technical Support
Date published: 2016-12-20

What fixation method do you recommend for IF (human fibroblasts)?

Asked by: Steven94
Thank you for your question. Our recommended fixation method for tissue culture cells is 5 minutes in -10° C methanol. Here is a link to our complete recommended protocol for immunofluorescence cell staining. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2016-11-14
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Rated 4 out of 5 by from it works good SC398822 it works at first try using 20ug mouse brain lysate. 1:1000 dilution is good.
Date published: 2020-07-02
Rated 4 out of 5 by from Buena señal. Detectamos solo la banda de LC3a; células estrelladas.
Date published: 2020-05-09
Rated 2 out of 5 by from Fair LC3BII strong, but LC3BI weak. Difficult to calculate ratio LC3BII:LC3BI
Date published: 2020-03-04
Rated 4 out of 5 by from works good in WB I used this antibody in WB in human fibroblasts. It showed a good signal for LC3I bot only weak for LC3II.
Date published: 2018-06-06
Rated 4 out of 5 by from Very good for ICC using rat primary neurons We used this antibody on rat primary neurons at a dilution of 1:50, the secondary was donkey anti-mouse Alexa 488 at 1:1000 we imaged at 40x. It worked well but the sc-271625 (G-2) worked the best for us.
Date published: 2018-04-11
Rated 5 out of 5 by from Good Antibody I just wanna try one anibody which is usually a little tricky, so I asked the staffs if I can get some free trial samples, they are very helpful and ship the samples to me by only one day. It works perfectly in my experiments, thank you so much for the company.
Date published: 2018-04-06
Rated 5 out of 5 by from Works Great.. I found this antibody as a free trial. it works really well in 20ug of whole cell lysate in N2A cells.
Date published: 2018-02-09
Rated 5 out of 5 by from 条带清晰,无杂带 申请的试用装。用的组织蛋白,第一次摸条件就出结果的。很棒!支持!申请的试用装。用的组织蛋白,第一次摸条件就出结果的。很棒!支持!申请的试用装。用的组织蛋白,第一次摸条件就出结果的。很棒!支持!
Date published: 2018-02-01
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