Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MAG Double Nickase Plasmid (h): sc-401604-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAG Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MAG Double Nickase Plasmid (h) and MAG Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MAG. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MAG Antibody (A-11): sc-166849
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAG Double Nickase Plasmid (h) | sc-401604-NIC | 20 µg | $410.00 | |||
MAG Double Nickase Plasmid (h2) | sc-401604-NIC-2 | 20 µg | $410.00 |
Myelin-associated glycoprotein (MAG) is a sialic acid–binding immunoglobulin-like cell surface protein enriched on myelinating glia, where it mediates axon–glia adhesion and stabilizes myelin–axon interactions. MAG engages glycan ligands and contributes to signaling that influences cytoskeletal organization, neurite extension, and maintenance of axonal integrity during nervous system development and adult homeostasis. In human cells, MAG-associated processes intersect with pathways governing myelin structure, axonal transport, and response to neural injury. Dysregulated MAG expression or signaling has been implicated in demyelinating and neurodegenerative pathophysiology and in mechanisms that limit axonal regeneration, making it a useful target for mechanistic studies in neurobiology.
MAG Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MAG locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MAG. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MAG function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MAG-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.