M13 Minor Coat Protein Antibody (E1): sc-57926
- M13 Minor Coat Protein Antibody (E1) is a mouse monoclonal IgG2a M13 Minor Coat Protein antibody provided at 100 µg/ml
- raised against isolated M13 bacteriophage coat proteins
- recommended for detection of minor coat protein (also designated as G3P) by WB
- See M13 Major Coat Protein (RL-ph1): sc-53004 for M13 Minor Coat Protein antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for M13 Minor Coat Protein Antibody (E1). This work is in progress.
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M13 Minor Coat Protein Antibody (E1) is a mouse monoclonal IgG2a antibody that detects M13 Minor Coat Protein of M13 phage origin by western blotting (WB). Anti-M13 Minor Coat Protein antibody (E1) is available as the non-conjugated format. M13 Minor Coat Protein, also known as attachment protein G3P, gene 3 protein (G3P), or III, is a 424 amino acid single-pass type I membrane protein that belongs to the inovirus G3P protein family. M13 Minor Coat Protein is crucial for the viral life cycle, facilitating the budding process and ensuring proper entry of the viral genome into bacterial hosts. M13 Minor Coat Protein forms a complex with G6P, which is essential for the correct termination of filamentous phage assembly. M13 Minor Coat Protein is located at the bacterial host inner membrane prior to assembly, and its structure consists of three distinct domains: N1, N2, and CT. The N1 domain is linked to N2 by a glycine-rich linker and interacts with tolA during infection, while the N2 domain associates with the F pilus, highlighting M13 Minor Coat Protein′s multifaceted role in the infection process. Anti-M13 Minor Coat Protein antibody (E1) is an invaluable tool for researchers studying phage biology and the mechanisms of viral infection.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
M13 Minor Coat Protein Antibody (E1) References:
- Filamentous phage infection: crystal structure of g3p in complex with its coreceptor, the C-terminal domain of TolA. | Lubkowski, J., et al. 1999. Structure. 7: 711-22. PMID: 10404600
- Nucleotide sequence of the genes III, VI and I of bacteriophage M13. | van Wezenbeek, P. and Schoenmakers, JG. 1979. Nucleic Acids Res. 6: 2799-818. PMID: 379830
- Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd. | van Wezenbeek, PM., et al. 1980. Gene. 11: 129-48. PMID: 6254849
- Construction and characterization of new coliphage M13 cloning vectors. | Hines, JC. and Ray, DS. 1980. Gene. 11: 207-18. PMID: 6260570
- The role of the adsorption complex in the termination of filamentous phage assembly. | Gailus, V., et al. 1994. Res Microbiol. 145: 699-709. PMID: 7746960
- The structural basis of phage display elucidated by the crystal structure of the N-terminal domains of g3p. | Lubkowski, J., et al. 1998. Nat Struct Biol. 5: 140-7. PMID: 9461080
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
M13 Minor Coat Protein Antibody (E1) | sc-57926 | 100 µg/ml | $322.00 |