M-RIP抗体 (E-1) 推荐用于 mouse, rat 和 human 来源的M-RIP WB, IP, IF 和 ELISA检测

M-RIP 抗体 (E-1): sc-515720

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说明书

    • 抗-M-RIP 抗体 E-1 是小鼠单克隆 IgG1 (kappa轻链),M-RIP抗体,规格为200 µg/ml
    • 免疫human来源M-RIP内部区域的668-760氨基酸
    • 抗-M-RIP 抗体 (E-1) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 M-RIP
    • 抗-M-RIP 抗体 (E-1) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取M-RIP (E-1): sc-515720免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for M-RIP Antibody (E-1) for WB applications. This reagent is now offered in a bundle with M-RIP Antibody (E-1) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

How much sample do you recommend loading on the gel for Western blot detection using M-RIP (E-1): sc-515720?

Asked by: Professor Griffin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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