Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LIS1 CRISPR/Cas9 KO Plasmid (m): sc-422102
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LIS1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LIS1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LIS1 Antibody (H-7): sc-374586
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LIS1 CRISPR/Cas9 KO Plasmid (m) | sc-422102 | 20 µg | $397.00 |
Overview
Pafah1b1 encodes LIS1, a dynein-binding regulatory protein that governs microtubule-dependent transport, centrosome positioning, and mitotic spindle organization. In mouse cells, LIS1 supports neuronal progenitor proliferation and neuronal migration by tuning cytoplasmic dynein–dynactin processivity and coupling cargo movement to the microtubule network. These functions integrate with cytoskeletal remodeling and cell cycle progression pathways that shape tissue architecture during development. Disruption of LIS1-associated processes is linked to neurodevelopmental phenotypes characterized by abnormal cortical layering and impaired neuronal positioning.
LIS1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pafah1b1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pafah1b1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pafah1b1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LIS1 protein expression.
This CRISPR knockout system enables efficient generation of Pafah1b1-deficient cell models for investigation of LIS1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pafah1b1 exon(s) critical for LIS1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pafah1b1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LIS1 CRISPR/Cas9 KO Plasmid (m) and LIS1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pafah1b1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LIS1 HDR Plasmid (m) and LIS1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pafah1b1 homology arms to support homology-directed repair at defined Pafah1b1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.