LIN-9 Double Nickase Plasmid (h): sc-418584-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LIN-9 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LIN-9 Double Nickase Plasmid (h) and LIN-9 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LIN9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LIN-9 Antibody (C-10): sc-398234
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LIN-9 Double Nickase Plasmid (h) | sc-418584-NIC | 20 µg | $410.00 | |||
LIN-9 Double Nickase Plasmid (h2) | sc-418584-NIC-2 | 20 µg | $410.00 |
LIN9 encodes LIN-9, a core component of the MuvB (MMB) transcriptional regulatory complex that coordinates cell-cycle gene expression. LIN-9 helps couple DREAM-mediated repression in quiescence with activation of G2/M transcription programs via interactions with B-MYB/FOXM1, supporting orderly DNA replication, mitotic progression, and chromosome stability. Through these roles, LIN-9 influences checkpoint control and proliferative capacity in diverse cell types. Dysregulation of MuvB/DREAM network components, including LIN9, is frequently investigated in the context of uncontrolled proliferation, genome instability, and tumor-associated cell-cycle transcription signatures.
LIN-9 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LIN9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LIN9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LIN9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LIN9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.