Date published: 2025-9-11

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Lex A 抗体 (2-12): sc-7544

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说明书
  • Lex A抗体(2-12)是小鼠单克隆IgG1 κ, 在79篇文献中引用,规格为200 µg/ml
  • 在Lex A的DNA结合域内的表位图谱
  • Lex A 抗体 (2-12) 推荐用于 WB, IP 和 IF,检测 Lex A and Lex A fusion proteins
  • 抗Lex A抗体(2-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Lex A (2-12): sc-7544免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Lex A Antibody (2-12) for WB applications. 的首选辅助检测试剂,这些试剂现与Lex A Antibody (2-12) 打包提供(请参阅下面的订购信息)。

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関連項目

Lex A 抗体(2-12)是一种小鼠单克隆 IgG1 kappa 轻链抗体,可用于检测 Lex A,包括在蛋白质印迹(WB)、免疫沉淀(IP)和免疫荧光(IF)等多种应用中。Anti-Lex A 抗体(2-12)有非结合型和多种结合型,如琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种 Alexa Fluor® 结合物,可满足不同实验需求。Lex A是一种参与基因表达调控的关键蛋白,在双杂交系统中扮演着重要角色,而双杂交系统是研究蛋白质-蛋白质相互作用的强大方法。该系统利用基因工程酵母或细菌,将"诱饵"蛋白与DNA结合域(DBD)融合,将"猎物"蛋白与转录激活域(AD)连接。这些蛋白质之间的相互作用会激活报告基因,使研究人员能够有效地识别和分析蛋白质相互作用。双杂交系统的适应性很强,它结合了GAL4或LexA等DNA结合域(DBD)以及GAL4或单纯疱疹病毒蛋白VP16的转录激活域(AD),因此成为蛋白质组学研究和相互作用组学绘图的最佳选择。利用原核蛋白(如大肠杆菌LexA的DBD)和酿酒酵母GAL4的N端的能力,增强了在各种生物体中进行实验的灵活性,确保了特定的转录激活和蛋白质在细胞内的适当定位。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Lex A 抗体 (2-12) 参考文献:

  1. 用于在酵母或哺乳动物细胞中表达的 GAL4 融合载体。  |  Sadowski, I., et al. 1992. Gene. 118: 137-41. PMID: 1511877
  2. 细菌 LexA 转录抑制物。  |  Butala, M., et al. 2009. Cell Mol Life Sci. 66: 82-93. PMID: 18726173
  3. 双杂交系统:一种识别和克隆与感兴趣的蛋白质相互作用的蛋白质基因的方法。  |  Chien, CT., et al. 1991. Proc Natl Acad Sci U S A. 88: 9578-82. PMID: 1946372
  4. 酵母 STE12 蛋白的信息素依赖性磷酸化与转录激活有关。  |  Song, D., et al. 1991. Genes Dev. 5: 741-50. PMID: 2026326
  5. 激活剂和目标。  |  Ptashne, M. and Gann, AA. 1990. Nature. 346: 329-31. PMID: 2142753
  6. 检测蛋白质-蛋白质相互作用的新型基因系统。  |  Fields, S. and Song, O. 1989. Nature. 340: 245-6. PMID: 2547163
  7. 移动遗传因子对 LexA 的使用和滥用。  |  Fornelos, N., et al. 2016. Trends Microbiol. 24: 391-401. PMID: 26970840
  8. 真菌基因调控机制模型:酿酒酵母的 GAL 基因。  |  Johnston, M. 1987. Microbiol Rev. 51: 458-76. PMID: 2830478
  9. GAL4 的缺失分析确定了两个转录激活区段。  |  Ma, J. and Ptashne, M. 1987. Cell. 48: 847-53. PMID: 3028647
  10. 靶向 LexA 自动裂解的纳米抗体揭示了一种抑制 SOS 反应途径的新策略。  |  Maso, L., et al. 2022. Structure. 30: 1479-1493.e9. PMID: 36240773
  11. 纯化的 lexA 蛋白是 recA 和 lexA 基因的抑制因子。  |  Little, JW., et al. 1981. Proc Natl Acad Sci U S A. 78: 4199-203. PMID: 7027255
  12. 双杂交系统:蛋白质-蛋白质相互作用的检测方法。  |  Fields, S. and Sternglanz, R. 1994. Trends Genet. 10: 286-92. PMID: 7940758

订购信息

产品名称产品编号规格价格数量收藏夹

Lex A 抗体 (2-12)

sc-7544
200 µg/ml
$316.00

Lex A (2-12): m-IgG Fc BP-HRP 套装

sc-526502
200 µg Ab; 10 µg BP
$354.00

Lex A (2-12): m-IgG1 BP-HRP 套装

sc-531875
200 µg Ab; 20 µg BP
$354.00

Lex A 抗体 (2-12) AC

sc-7544 AC
500 µg/ml, 25% agarose
$416.00

Lex A 抗体 (2-12) HRP

sc-7544 HRP
200 µg/ml
$316.00

Lex A 抗体 (2-12) FITC

sc-7544 FITC
200 µg/ml
$330.00

Lex A 抗体 (2-12) PE

sc-7544 PE
200 µg/ml
$343.00

Lex A 抗体 (2-12) Alexa Fluor® 488

sc-7544 AF488
200 µg/ml
$357.00

Lex A 抗体 (2-12) Alexa Fluor® 546

sc-7544 AF546
200 µg/ml
$357.00

Lex A 抗体 (2-12) Alexa Fluor® 594

sc-7544 AF594
200 µg/ml
$357.00

Lex A 抗体 (2-12) Alexa Fluor® 647

sc-7544 AF647
200 µg/ml
$357.00

Lex A 抗体 (2-12) Alexa Fluor® 680

sc-7544 AF680
200 µg/ml
$357.00

Lex A 抗体 (2-12) Alexa Fluor® 790

sc-7544 AF790
200 µg/ml
$357.00

What is this Lex A antibody raised against?

Asked by: cjMara
This antibody is raised agsinst a LexA-VP16 fusion protein mapping within the DNA binding domain of Lex A.
Answered by: Technical Support 7
Date published: 2017-01-18
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Rated 5 out of 5 by from Ottimo funzionamentoHo provato un campione per la detezione di un Lex A tag in lieviti e ha funzionato. Ho utilizzato il campione in una diluzione 1:500.
Date published: 2019-03-27
Rated 5 out of 5 by from Great WBVery clear western blot data; strong signal with low background
Date published: 2017-02-01
Rated 5 out of 5 by from Recommended for WBWell cited antibody recommended for detection of Lex A and Lex A fusion proteins by WB, IP and IF.
Date published: 2016-12-23
Rated 3 out of 5 by from Worked well for western blotting using L40Worked well for western blotting using L40 yeast cell lysates. -SCBT Publication Review
Date published: 2015-05-08
Rated 3 out of 5 by from Positive results in western blot analysisPositive results in western blot analysis of recombinant VP16-Lex A and Lex A proteins and untransformed E. coli cell lysate. -SCBT QC
Date published: 2015-02-22
Rated 4 out of 5 by from Used for western blot studies with transfected EUsed for western blot studies with transfected E. Coli cells. -SCBT Publication Review
Date published: 2015-02-19
Rated 4 out of 5 by from WB with human C33A cell lysatesWB with human C33A cell lysates. -SCBT Publication Review
Date published: 2015-01-07
Rated 4 out of 5 by from Published western blot data using yeast cellPublished western blot data using yeast cell lysates: EGY40, EGY42, and EGY48. -SCBT Publication Review
Date published: 2013-09-08
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Lex A 抗体 (2-12) is rated 4.1 out of 5 by 8.
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