Date published: 2025-9-6
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Lamin A/C Antibody (E-1) - Western Blotting - Image 390725
Lamin A/C 抗体 (E-1) Alexa Fluor® 594: sc-376248 AF594. C32 (A), PC-3 (B), HeLa (C), A-431 (D), NIH/3T3 (E) 和 Sol8 (F) 全细胞裂解液中 Lamin A/C 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
Lamin A/C 抗体 (E-1) Alexa Fluor® 594: sc-376248 AF594. C32 (A), PC-3 (B), HeLa (C), A-431 (D), NIH/3T3 (E) 和 Sol8 (F) 全细胞裂解液中 Lamin A/C 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

Lamin A/C 抗体 (E-1): sc-376248

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说明书
  • Lamin A/C 抗体 E-1 是小鼠单克隆 IgG1 κ，Lamin A/C抗体, 在280篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Lamin A/C的N-terminus的氨基酸2-29之间
  • Lamin A/C 抗体 (E-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Lamin A and Lamin C; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗Lamin A/C抗体（E-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Lamin A/C (E-1): sc-376248的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是Lamin A/C Antibody (E-1) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂，这些试剂现与Lamin A/C Antibody (E-1) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

附加Lamin抗体，包括Lamin A, Lamin B1, Lamin B2和Lamin C

Lamin A/C Antibody (E-1) 是一种 IgG1 κ 小鼠单克隆 Lamin A/C 抗体（也称为 LMNA 抗体或 LMNC 抗体），可通过 WB、IP、IF、IHC(P) 和 ELISA 检测小鼠、大鼠和人类来源的 Lamin A/C 蛋白。Lamin A/C Antibody (E-1) 既可作为非偶联的抗 Lamin A/C 抗体形式提供，也可作为多种偶联形式的抗 Lamin A/C 抗体提供，包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。已描述了一个独特的半胱氨酸蛋白酶家族，该家族在序列、结构和底物特异性上与以往描述的任何蛋白酶家族都不同。这个被称为 Ced-3/ICE 的家族由 ICE、CPP32、ICH-1/Nedd-2、Tx、Mch2、Mch3（ICE-LAP3 或 CMH-1）、Mch4 和 ICE-LAP6 组成。Ced-3/ICE 家族成员是细胞凋亡机制的关键组成部分，并起着破坏对细胞寿命至关重要的特定靶蛋白的作用。核纤层蛋白对于维持核膜和细胞形态的完整性至关重要。核 Lamin A 被 Mch2 切割，而不是 CPP32。核 Lamin B 因凋亡而被 ICE 家族的一个未知成员切割。Lamin C 是 Lamin A 的剪接变体，仅在羧基末端有所不同。Lamin A 和 C 的前 566 个氨基酸是相同的，而 Lamin C 仅在六个独特的羧基末端氨基酸上有所不同。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Lamin A/C 抗体 (E-1) 参考文献:

  1. 小鼠精子发生过程中精子顶轴体中的Lamin A/C蛋白至关重要  |  Shen, J., et al. 2014. Reproduction. 148: 479-87. PMID: 25118303
  2. 依赖于 Lamin A/C 的 53BP1 相互作用促进了细胞对 DNA 损伤的反应。  |  Gibbs-Seymour, I., et al. 2015. Aging Cell. 14: 162-9. PMID: 25645366
  3. Lamin A/C 可维持 PcG 蛋白结构，维持目标基因的转录抑制。  |  Cesarini, E., et al. 2015. J Cell Biol. 211: 533-51. PMID: 26553927
  4. Lamin A/C 在早期乳腺癌中的表达及其预后价值  |  Alhudiri, IM., et al. 2019. Breast Cancer Res Treat. 174: 661-668. PMID: 30610489
  5. PCAF 参与肌肉分化早期阶段 Lamin A/C-HDAC2 的相互作用  |  Santi, S., et al. 2020. Cells. 9: PMID: 32698523
  6. 片层蛋白 A/C错义变异：从发现到功能验证。  |  Lazarte, J. and Hegele, RA. 2021. NPJ Genom Med. 6: 102. PMID: 34862397
  7. 基质重塑控制着核片层蛋白A/C-emerin网络，该网络引导着Wnt调控的干细胞命运。  |  Tang, Y., et al. 2022. Dev Cell. 57: 480-495.e6. PMID: 35150612
  8. BAF A12T突变会破坏层粘连A/C的相互作用，从而影响Nestor-Guillermo早衰综合征细胞核包膜破裂的稳健修复。  |  Janssen, A., et al. 2022. Nucleic Acids Res. 50: 9260-9278. PMID: 36039758
  9. 在微尺度受限微环境中，Lamin A/C和Vimentin作为变形虫迁移过程中的协调调节因子。  |  Wang, YJ., et al. 2023. Nano Lett. 23: 6727-6735. PMID: 37459599
  10. 细胞核中的 Lamin A/C和PI(4,5)P2--新型复合物  |  Escudeiro-Lopes, S., et al. 2024. Cells. 13: PMID: 38474363

订购信息

产品名称产品编号规格价格数量收藏夹

Lamin A/C 抗体 (E-1)

sc-376248
200 µg/ml
$316.00

Lamin A/C (E-1): m-IgG Fc BP-HRP 套装

sc-529844
200 µg Ab; 10 µg BP
$354.00

Lamin A/C (E-1): m-IgGκ BP-HRP 套装

sc-522892
200 µg Ab, 40 µg BP
$354.00

Lamin A/C 抗体 (E-1) AC

sc-376248 AC
500 µg/ml, 25% agarose
$416.00

Lamin A/C 抗体 (E-1) HRP

sc-376248 HRP
200 µg/ml
$316.00

Lamin A/C 抗体 (E-1) FITC

sc-376248 FITC
200 µg/ml
$330.00

Lamin A/C 抗体 (E-1) PE

sc-376248 PE
200 µg/ml
$343.00

Lamin A/C 抗体 (E-1) Alexa Fluor® 488

sc-376248 AF488
200 µg/ml
$357.00

Lamin A/C 抗体 (E-1) Alexa Fluor® 546

sc-376248 AF546
200 µg/ml
$357.00

Lamin A/C 抗体 (E-1) Alexa Fluor® 594

sc-376248 AF594
200 µg/ml
$357.00

Lamin A/C 抗体 (E-1) Alexa Fluor® 647

sc-376248 AF647
200 µg/ml
$357.00

Lamin A/C 抗体 (E-1) Alexa Fluor® 680

sc-376248 AF680
200 µg/ml
$357.00

Lamin A/C 抗体 (E-1) Alexa Fluor® 790

sc-376248 AF790
200 µg/ml
$357.00

Lamin A/C (E-1) 中和勝肽

sc-376248 P
100 µg/0.5 ml
$68.00

Hi, I want to try out this ab and would like to get the free sample first to see whether it will work for my experiments, would it be possible to receive this? thanks!

Asked by: Anonymous
Thank you for your question. Please contact technical service to arrange for a free vial of this antibody.
Answered by: Tech Support Europe
Date published: 2025-06-04

Does this antibody recognize progerin?

Asked by: Cristinadpr
Thank you for your question. Progerin has a C-terminal 50 amino acid deletion. As this antibody recognizes the N-terminus of Lamin, it should also be able to detect Progerin.
Answered by: Tech Support Europe
Date published: 2021-02-24

What retrieval would you suggest for the staining of paraffin sections?

Asked by: RS777
Thank you for your question. You can find this information in our recommended protocol, which is available here: https://www.scbt.com/resources/protocols/immunoperoxidase-staining Our recommended method is heat treatment. Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides.
Answered by: Technical Support Europe
Date published: 2020-10-12

I have seen that LMNA antibody (N-18, sc-6215) has been used in several publications related to IPSC-derived cardiomyocytes. Since this product has been discontinued, I would like to know the similarities or differences of this antibody and sc-6215.Thanks

Asked by: lywong33
Thank you for your question. Both antibodies are made against a peptide mapping at the N terminus of Lamin A/C of human origin (accession#P02545). sc-376248 is an excellent mouse monoclonal antibody that is recommended for the same applications.
Answered by: Tech Service
Date published: 2020-05-29

I received a sample of lamin A/C (E-1) (sc-376248); what is the recommended secondary for this? I am using it to blot for mouse lamin.

Asked by: Amonta
Thank you for your question. This antibody is a mouse monoclonal IgG1 (kappa light chain). The recommended secondary is our m-IgGκ BP-HRP: sc-516102, mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP). For other conjugates, please check on our website.
Answered by: Technical Support
Date published: 2019-12-07

Which blocking agent do you recommend for the dilution of this antibody? BSA or non-fat milk 5%?

Asked by: VirL
Thank you for your question. We recommend using 5% nonfat milk in TBST. We provide this pre-made as our TBS Blotto A: sc-2333. https://www.scbt.com/scbt/product/tbs-blotto-a
Answered by: Technical Support
Date published: 2021-09-21

Can you recommend a negative control to use with Lamin A/C Antibody (E-1): sc-376248?

Asked by: jenni
We recommend using the blocking peptide sc-376248 P as a negative control. Antibody binding to antigen may be blocked/competed by pre-absorption with the blocking peptide. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 6
Date published: 2017-01-09
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Rated 5 out of 5 by from Lamin A/C antibody works well for WBAntibody works well for WB at 1/1000 for MDA-468 cells, BT-549 cells, MDA-231 cells, PyMT cells, and Met1 cells.
Date published: 2023-12-06
Rated 5 out of 5 by from 很好用上个月购买此产品 用起来很好，后面会给同行推荐，希望有越来越多的人能够购买此产品，为我们的科研事业助力！
Date published: 2021-10-08
Rated 5 out of 5 by from Works very good in WB, IF and IHCVery good results in WB and immunofluorescence (4% PFA fixation) in mouse cells and in IHC on mouse and human bone samples.
Date published: 2021-06-25
Rated 4 out of 5 by from good for IFI used sc-376248 on 96 wells fixed in 4%PFA. It works very well (IF)
Date published: 2021-06-21
Rated 4 out of 5 by from Useful for IFI compared E-1 (sc-376248) and 346 (sc-7293) for IF of both MEF wt and MEF Lmna-/-. E-1 are useful for IF, but slightly labels other nuclear proteins in our condition (4%PFA 15min fixation followed 1:500 dilution / 4C overnight reaction).
Date published: 2021-03-07
Rated 4 out of 5 by from Lamin A/C (E-1) for WB Good detectionI have bought this product a month ago and it is working perfectly for WB. Bands are sharp at the correct molecular weight, a bit of nonspecific signal at lower molecular weight but good resolution.
Date published: 2020-05-19
Rated 4 out of 5 by from good antibodywe tested it on WB of total lysates from mouse embryonic hearts ( 40 micrograms). Antibody was diltuted 1:300. we got good and specific signal
Date published: 2019-11-14
Rated 5 out of 5 by from Beautiful myonuclear envelope labelLamin A/C (green) 1:100 following ice cold acetone fixation of fresh frozen human muscle tissue. Secondary antibody was goat anti-mouse IgG1 AlexaFluor 488 (1:200). Staining was on sections previously stained with Laminin (also in green) and DAPI (blue) to identify myonuclei. This antibody provided beautiful staining with little effort!
Date published: 2019-07-31
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Lamin A/C 抗体 (E-1) is rated 4.8 out of 5 by 60.
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