Date published: 2025-9-7
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KIF2A Antibody (D-7) - Western Blotting - Image 300152
KIF2A 抗体 (D-7): sc-271471. HeLa (A), Hep G2 (B) 和 NIH/3T3 (C) 全细胞裂解液中 KIF2A 表达的 Western 印迹分析.
KIF2A 抗体 (D-7): sc-271471. HeLa (A), Hep G2 (B) 和 NIH/3T3 (C) 全细胞裂解液中 KIF2A 表达的 Western 印迹分析.

KIF2A 抗体 (D-7): sc-271471

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说明书
  • KIF2A 抗体 D-7 是小鼠单克隆 IgG1 κ，KIF2A抗体, 在3篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的KIF2A的C-terminus附近的氨基酸528-569之间
  • KIF2A 抗体 (D-7) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 KIF2A
  • 抗KIF2A抗体（D-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取KIF2A (D-7): sc-271471的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是KIF2A Antibody (D-7) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与KIF2A Antibody (D-7) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外KIF抗体，包括KIF12, KIF13B, KIF14, KIF15, KIF17, KIF18A, KIF1B, KIF20A, KIF21B, KIF26A, KIF27, KIF2A, KIF2C, KIF3A, KIF3B和KIF3C

KIF2A抗体（D-7）是一种IgG1 κ小鼠单克隆KIF2A抗体（也称为Kinesin-2抗体或KNS2抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人的KIF2A蛋白。KIF2A抗体（D-7）既可以是非偶联的抗KIF2A抗体形式，也可以是多种偶联形式的抗KIF2A抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Kinesin是一种细胞骨架马达蛋白，参与轴突运输和细胞分裂。驱动蛋白超家族蛋白（KIFs）是马达蛋白，它们沿着微管运输对发育中神经元的轴突延伸重要的囊泡，例如大分子和膜性细胞器。KIF参与神经元功能和发展。驱动蛋白重链成员2A（KIF2A），也称为KNS2，是一种与微管相关的中心型马达蛋白，属于类驱动蛋白家族。KIF2A在发育中的轴突中大量存在。合成类视黄醇N-(4-羟基苯基)-全反式视黄酰胺HPR是一种体内癌症化学预防剂和体外凋亡细胞死亡诱导剂，可调节KIF2A。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

KIF2A 抗体 (D-7) 参考文献:

  1. 驱动蛋白超家族蛋白 2A（KIF2A）在抑制侧枝延伸方面的功能  |  Homma, N., et al. 2003. Cell. 114: 229-39. PMID: 12887924
  2. 驱动蛋白超家族蛋白及其各种功能和动力学。  |  Hirokawa, N. and Takemura, R. 2004. Exp Cell Res. 301: 50-9. PMID: 15501445
  3. 利用 cDNA 差示技术识别新型人类驱动蛋白相关基因（HK2）。  |  Debernardi, S., et al. 1997. Genomics. 42: 67-73. PMID: 9177777
  4. 抑制 PC12 细胞中的 KIF2 会改变生长锥非突触膜受体的分布并抑制神经元的延伸。  |  Morfini, G., et al. 1997. J Cell Biol. 138: 657-69. PMID: 9245793

订购信息

产品名称产品编号规格价格数量收藏夹

KIF2A 抗体 (D-7)

sc-271471
200 µg/ml
$316.00

KIF2A (D-7): m-IgG Fc BP-HRP 套装

sc-529191
200 µg Ab; 10 µg BP
$354.00

KIF2A (D-7): m-IgGκ BP-HRP 套装

sc-521952
200 µg Ab, 40 µg BP
$354.00

KIF2A (D-7): m-IgG1 BP-HRP 套装

sc-543378
200 µg Ab; 20 µg BP
$354.00

KIF2A 抗体 (D-7) AC

sc-271471 AC
500 µg/ml, 25% agarose
$416.00

KIF2A 抗体 (D-7) HRP

sc-271471 HRP
200 µg/ml
$316.00

KIF2A 抗体 (D-7) FITC

sc-271471 FITC
200 µg/ml
$330.00

KIF2A 抗体 (D-7) PE

sc-271471 PE
200 µg/ml
$343.00

KIF2A 抗体 (D-7) Alexa Fluor® 488

sc-271471 AF488
200 µg/ml
$357.00

KIF2A 抗体 (D-7) Alexa Fluor® 546

sc-271471 AF546
200 µg/ml
$357.00

KIF2A 抗体 (D-7) Alexa Fluor® 594

sc-271471 AF594
200 µg/ml
$357.00

KIF2A 抗体 (D-7) Alexa Fluor® 647

sc-271471 AF647
200 µg/ml
$357.00

KIF2A 抗体 (D-7) Alexa Fluor® 680

sc-271471 AF680
200 µg/ml
$357.00

KIF2A 抗体 (D-7) Alexa Fluor® 790

sc-271471 AF790
200 µg/ml
$357.00

KIF2A (D-7) 中和勝肽

sc-271471 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-271471 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good antibodyI got a free sample and it works well.Good signal.
Date published: 2019-04-22
Rated 4 out of 5 by from Good for IFThis KIF2A Antibody (D-7) shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2018-02-20
Rated 4 out of 5 by from a good antibody for WBThis antibody recognizes a clear signal at the predicted M.W. on WB (1:500 dilution, 40 ug HeLa extract).
Date published: 2017-09-03
Rated 3 out of 5 by from Kif2A reviewThe antibody do not work very well diluted at 1:100 in Hela and A549 whole cell lysates.
Date published: 2017-07-11
Rated 5 out of 5 by from Conclusive performance on Cultures; WB, and IFWB: OK (good signal at 1:200 dilution) IF: OK (likely detecting KIF2A under all fixation conditions, including formaldehyde, methanol, formaldehyde followed by methanol, but also producing noise staining)
Date published: 2017-02-08
Rated 1 out of 5 by from Poor results by IP (only IP 0Poor results by IP (only IP 0.1% of the total KIF2A at 5 g/ml).
Date published: 2015-09-09
Rated 4 out of 5 by from WB: Good signal at 1:200 dilution; IF:WB: Good signal at 1:200 dilution; IF: OK (likely detecting KIF2A under all fixation conditions, including formaldehyde, methanol, formaldehyde followed by methanol, but also producing noise staining)
Date published: 2015-09-09
Rated 5 out of 5 by from Good specificity in WB in mouse brain tissueGood specificity in WB in mouse brain tissue extract, SK-N-MC and H4 whole cell lysates. -SCBT QC
Date published: 2015-03-10
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KIF2A 抗体 (D-7) is rated 4.0 out of 5 by 10.
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