Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
karyopherin β2 Double Nickase Plasmid (h): sc-403099-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- karyopherin β2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- karyopherin β2 Double Nickase Plasmid (h) and karyopherin β2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TNPO1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: karyopherin β2 Antibody (F-6): sc-166127
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
karyopherin β2 Double Nickase Plasmid (h) | sc-403099-NIC | 20 µg | $410.00 | |||
karyopherin β2 Double Nickase Plasmid (h2) | sc-403099-NIC-2 | 20 µg | $410.00 |
TNPO1 encodes karyopherin β2 (transportin-1), a RanGTP-regulated nuclear import receptor that recognizes PY-type nuclear localization signals and mediates selective transport of RNA-binding proteins and other cargos through the nuclear pore complex. This import pathway supports RNA metabolism, stress granule dynamics, and nucleo-cytoplasmic partitioning of regulatory factors, linking TNPO1 activity to gene expression control and proteostasis. Altered transportin-dependent trafficking has been associated with disturbed RNA processing and protein aggregation phenotypes observed in neurodegeneration-related biology, as well as with proliferative signaling programs in cancer models. As a result, TNPO1 is frequently studied to dissect mechanisms of nuclear transport specificity, Ran cycle coupling, and how mislocalized RBPs impact cellular homeostasis.
karyopherin β2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TNPO1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TNPO1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TNPO1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TNPO1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.