JIP-3 Antibody (D-3): sc-48392

JIP-3 Antibody (D-3) is a mouse monoclonal IgG1 antibody that detects JIP-3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). JIP-3, also known as JSAP1 or MAPK8IP3, is a 1,336 amino acid protein that plays a crucial role in the JNK signaling pathway by acting as a scaffold that organizes various components of the mitogen-activated protein kinase cascade. This organization is vital for proper JNK signaling, which drives numerous cellular processes, including stress responses, apoptosis, and neuronal development. JIP-3 is predominantly found in the cytoplasm and expressed in various tissues, including the brain and heart, where JIP-3 forms homo- or heterooligomeric complexes that interact with key signaling molecules. JIP-3 (D-3) antibody helps researchers study how JIP-3 regulates kinesin-dependent axonal transport, which is essential for neuronal function and communication. JIP-3 may undergo phosphorylation in response to DNA damage, highlighting its potential role in cellular stress responses. With 69% similarity to its mouse counterpart, human JIP-3 underscores the evolutionary conservation of its function across species, and the existence of multiple isoforms due to alternative splicing further emphasizes the complexity and regulatory potential of this important protein. Anti-JIP-3 antibody (D-3) serves as an invaluable tool for researchers studying the intricacies of JNK signaling and its implications in health and disease.

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JIP-3 Antibody (D-3) References:

1. A JIP3-regulated GSK3β/DCX signaling pathway restricts axon branching.  |  Bilimoria, PM., et al. 2010. J Neurosci. 30: 16766-76. PMID: 21159948
2. JIP3 Activates Kinesin-1 Motility to Promote Axon Elongation.  |  Watt, D., et al. 2015. J Biol Chem. 290: 15512-15525. PMID: 25944905
3. ARF6-JIP3/4 regulate endosomal tubules for MT1-MMP exocytosis in cancer invasion.  |  Marchesin, V., et al. 2015. J Cell Biol. 211: 339-58. PMID: 26504170
4. JIP3 regulates neuronal radial migration by mediating TrkB axonal anterograde transport in the developing cerebral cortex.  |  Ma, H., et al. 2017. Biochem Biophys Res Commun. 485: 790-795. PMID: 28259553
5. Impaired JIP3-dependent axonal lysosome transport promotes amyloid plaque pathology.  |  Gowrishankar, S., et al. 2017. J Cell Biol. 216: 3291-3305. PMID: 28784610
6. JIP3 knockout protects mice against high fat diet-induced liver injury.  |  Ma, XJ., et al. 2018. Biochem Biophys Res Commun. 497: 819-826. PMID: 29454969
7. JIP3 deficiency attenuates cardiac hypertrophy by suppression of JNK pathway.  |  Ma, Q., et al. 2018. Biochem Biophys Res Commun. 503: 1-7. PMID: 29604277
8. Insights into Kinesin-1 Activation from the Crystal Structure of KLC2 Bound to JIP3.  |  Cockburn, JJB., et al. 2018. Structure. 26: 1486-1498.e6. PMID: 30197037
9. Doublecortin and JIP3 are neural-specific counteracting regulators of dynein-mediated retrograde trafficking.  |  Fu, X., et al. 2022. Elife. 11: PMID: 36476638
10. Axonal transport of autophagosomes is regulated by dynein activators JIP3/JIP4 and ARF/RAB GTPases.  |  Cason, SE. and Holzbaur, ELF. 2023. J Cell Biol. 222: PMID: 37909920

The preferred JIP-3 antibody is JIP-3 Antibody (F-6): sc-46663.