JAM-A Double Nickase Plasmid (m): sc-421202-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- JAM-A Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- JAM-A Double Nickase Plasmid (m) and JAM-A Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting F11r. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: JAM-A Antibody (J10.4): sc-53623
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
JAM-A Double Nickase Plasmid (m) | sc-421202-NIC | 20 µg | $410.00 | |||
JAM-A Double Nickase Plasmid (m2) | sc-421202-NIC-2 | 20 µg | $410.00 |
Mouse F11r encodes junctional adhesion molecule A (JAM-A), an immunoglobulin superfamily protein concentrated at tight junctions where it supports epithelial and endothelial barrier integrity and cell polarity. JAM-A participates in junctional assembly and remodeling through interactions with scaffold proteins such as ZO-1 and PDZ-domain partners, influencing processes including paracellular permeability and contact-dependent signaling. In leukocytes and vascular endothelium, JAM-A contributes to adhesion and transmigration dynamics, linking junctional organization to inflammatory trafficking. Dysregulated JAM-A/F11r expression or localization has been associated with barrier dysfunction phenotypes relevant to inflammation, vascular pathology, and tumor cell invasion models.
JAM-A Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the F11r locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within F11r. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt F11r function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of F11r-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.