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JAM-A Antibody (1H2A9) - Western Blotting - Image 13983 — JAM-A 抗体 (1H2A9): sc-53624. 还原条件 (A, B, C) 和非还原条件 (D, E, F) 下, 人血小板 (A, D), 人 PBL (B, E) 和 T84 (C, F) 全细胞裂解液中 JAM-A 表达的 Western 印迹分析.

JAM-A 抗体 (1H2A9): sc-53624. 还原条件 (A, B, C) 和非还原条件 (D, E, F) 下, 人血小板 (A, D), 人 PBL (B, E) 和 T84 (C, F) 全细胞裂解液中 JAM-A 表达的 Western 印迹分析.

JAM-A 抗体 (1H2A9): sc-53624

Name: JAM-A抗体 (1H2A9)
Brand: SCBT
SKU: sc-53624
Price: 316.0 USD
Availability: InStock

5.0(6)

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说明书

JAM-A抗体(1H2A9)是小鼠单克隆IgG_2b κ, 在8篇文献中引用，规格为200 µg/ml
针对human的重组JAM融合蛋白的抗体
JAM-A 抗体 (1H2A9) 推荐用于WB, IP, IF 和 FCM，检测human 来源的 JAM-A
抗JAM-A抗体（1H2A9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取JAM-A (1H2A9): sc-53624的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP是JAM-A Antibody (1H2A9) 适用于 WB 应用。的首选辅助检测试剂。该试剂现与JAM-A Antibody (1H2A9) 搭配使用（请参阅下面的订购信息）。

查看产品引用文献(8)

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関連項目

附加JAM抗体，包括JAM-A, JAM-B, JAM-C和JAM4

JAM-A抗体（1H2A9）是一种IgG2b κ小鼠单克隆JAM-A抗体，可通过WB、IP、IF和FCM检测人源的JAM-A。JAM-A抗体（1H2A9）可作为非偶联抗JAM-A抗体形式提供，也可作为多种偶联形式的抗JAM-A抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。连接粘附分子（JAM）是免疫球蛋白超家族的成员，表达于上皮细胞和内皮细胞的紧密连接处。它与白细胞的跨内皮迁移有关。JAM在循环单核细胞、中性粒细胞、淋巴细胞亚群和血小板上持续表达。JAM家族由JAM-A、JAM-B和JAM-C组成，也可分别命名为JAM-1、JAM-2和JAM-3。JAM-A通过与F-Actin的定位在细胞-细胞接触处和膜皱褶处。它通过同型相互作用参与细胞间粘附，并在组织紧密连接和调节白细胞渗出方面发挥作用。JAM-B与离散的PBL亚群相互作用，表明它可能在淋巴细胞迁移中发挥作用。JAM-B和JAM-C蛋白是结合伙伴；JAM-C可能是功能性JAM-B受体。具体来说，JAM-B通过与JAM-C的异型相互作用粘附到T细胞上。JAM-B/JAM-C相互作用可能在T细胞、NK细胞和树突状细胞炎症中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor^® 是Molecular Probes Inc., OR., USA的商标

LI-COR^®和 Odyssey^® 是LI-COR Biosciences的注册商标

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
JAM-A 抗体 (1H2A9)	sc-53624	200 µg/ml	$316.00
JAM-A (1H2A9): m-IgG Fc BP-HRP 套装	sc-525613	200 µg Ab; 10 µg BP	$354.00
JAM-A 抗体 (1H2A9) AC	sc-53624 AC	500 µg/ml, 25% agarose	$416.00
JAM-A 抗体 (1H2A9) HRP	sc-53624 HRP	200 µg/ml	$316.00
JAM-A 抗体 (1H2A9) FITC	sc-53624 FITC	200 µg/ml	$330.00
JAM-A 抗体 (1H2A9) PE	sc-53624 PE	200 µg/ml	$343.00
JAM-A 抗体 (1H2A9) Alexa Fluor^® 488	sc-53624 AF488	200 µg/ml	$357.00
JAM-A 抗体 (1H2A9) Alexa Fluor^® 546	sc-53624 AF546	200 µg/ml	$357.00
JAM-A 抗体 (1H2A9) Alexa Fluor^® 594	sc-53624 AF594	200 µg/ml	$357.00
JAM-A 抗体 (1H2A9) Alexa Fluor^® 647	sc-53624 AF647	200 µg/ml	$357.00
JAM-A 抗体 (1H2A9) Alexa Fluor^® 680	sc-53624 AF680	200 µg/ml	$357.00
JAM-A 抗体 (1H2A9) Alexa Fluor^® 790	sc-53624 AF790	200 µg/ml	$357.00

human JAM-A抗体的克隆号就是括号里面的1H2A9吗？

Asked by: 骄阳似火

Thank you for your inquiry. Yes，1H2A9 is clone name. 感谢您的提问。1H2A9是克隆名。

Answered by: Technical Support 9

Date published: 2020-03-25

Can JAM-A (1H2A9): sc-53624 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp

Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, JAM-A (1H2A9): sc-53624 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.

Answered by: Technical Support

Date published: 2017-02-24

Rated 5 out of 5 by jerojero from Excellent!This antibody works great in western blot analysis of JAM-A expression in human platelet, human PBL and T84 whole cell lysates under reducing and non-reducing conditions.

Date published: 2017-01-19

Rated 5 out of 5 by LF from Strong band in WB in human platelet extractStrong band in WB in human platelet extract, human PBL and T84 whole cell lysates. -SCBT QC

Date published: 2015-06-01

Rated 5 out of 5 by TO from Produced excellent indirect FCM data forProduced excellent indirect FCM data for human peripheral blood leukocytes stained with JAM-A . -SCBT QC

Date published: 2015-03-31

Rated 5 out of 5 by SM from IF publishable data with human and dog andIF publishable data with human and dog and hamster A549, HeLa, MDCK, and CHO - SCBT Publication Review

Date published: 2015-03-25

Rated 5 out of 5 by SH from Good WB publishable data gathered with HUVECsGood WB publishable data gathered with HUVECs, human pulmonary arterial ECs (HPAECs), and HSaVECs. -SCBT Publication Review

Date published: 2015-03-25

Rated 5 out of 5 by JT from Beautiful immunofluorescence membrane stainingBeautiful immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC

Date published: 2015-03-13

JAM-A 抗体 (1H2A9) is rated 5.0 out of 5 by 6.

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JAM-A 抗体 (1H2A9): sc-53624

JAM-A 抗体 (1H2A9) 规格

替代产品名称: JAM-A

产品克隆名称: 1H2A9

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG_2b kappa light chain

氨基酸:

抗原表位靶标: JAM

抗原表位种属: human

产品反应性/检测: JAM-A

种属反应性/检测: human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, FCM

瓶的信息: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 8篇各种科学出版物的引文

JAM-A 抗体 (1H2A9)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

優選的 JAM-A 抗體是 JAM-A 抗體 (J10.4): sc-53623.