IRX4 CRISPR/Cas9 KO Plasmid (h): sc-405618
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IRX4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IRX4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IRX4 CRISPR/Cas9 KO Plasmid (h) | sc-405618 | 20 µg | $397.00 |
Overview
IRX4 (Iroquois homeobox 4) encodes a homeodomain transcription factor that regulates spatiotemporal gene expression programs during development, with prominent roles in cardiac and other mesodermal lineages. As a nuclear DNA-binding protein, IRX4 contributes to transcriptional networks that shape cell fate decisions, differentiation, and tissue patterning by modulating downstream target genes and interacting with broader homeobox-regulated pathways. Dysregulated IRX4 expression or regulatory circuitry has been implicated in altered developmental signaling and context-dependent transcriptional states observed in cancer biology, making it relevant for studies of lineage specification and disease-associated gene regulation.
IRX4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IRX4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IRX4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IRX4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IRX4 protein expression.
This CRISPR knockout system enables efficient generation of IRX4-deficient cell models for investigation of IRX4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IRX4 exon(s) critical for IRX4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IRX4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IRX4 CRISPR/Cas9 KO Plasmid (h) and IRX4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IRX4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IRX4 HDR Plasmid (h) and IRX4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IRX4 homology arms to support homology-directed repair at defined IRX4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.