Date published: 2025-9-8
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IRS-4 Antibody (RI-7) - Western Blotting - Image 34226
IRS-4 抗体 (RI-7): sc-100854. 对 HeLa 全细胞裂解液中 IRS-4 的表达进行 Western 印迹分析.
IRS-4 Antibody (RI-7) - Western Blotting - Image 34226
IRS-4 抗体 (RI-7): sc-100854. 对 HeLa 全细胞裂解液中 IRS-4 的表达进行 Western 印迹分析.
IRS-4 Antibody (RI-7) - Western Blotting - Image 135787
IRS-4 抗体 (RI-7): sc-100854. 非转染: sc-117752 (A) 和人 IRS-4 转染: sc-176236 (B) 293T 全细胞裂解液中 IRS-4 表达的 Western 印迹分析.
IRS-4 抗体 (RI-7): sc-100854. 对 HeLa 全细胞裂解液中 IRS-4 的表达进行 Western 印迹分析.

IRS-4 抗体 (RI-7): sc-100854

4.5(4)
寫評論提問

说明书
  • IRS-4抗体(RI-7)是小鼠单克隆IgG1 κ, 在4篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组IRS-4
  • 推荐用于 human 来源的IRS-4 WB, IP 和 ELISA检测
  • 关于如何获取IRS-4 (RI-7): sc-100854的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成IRS-4 抗体 (RI-7)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

此外 IRS 抗体包括 IRS-1, IRS-2 和 IRS-4

IRS-4抗体（RI-7）是一种IgG1κ小鼠单克隆IRS-4抗体，可通过WB、IP和ELISA检测人源IRS-4。IRS-4抗体（RI-7）可作为非偶联抗IRS-4抗体形式提供。胰岛素受体底物（IRS）蛋白是胰岛素受体信号传导的关键成分。IRS-4是IRS家族中最新鉴定的成员，具有未确定的体内功能。磷酸化的IRS-4与磷脂酰肌醇3激酶（PI3-激酶）结合，参与胰岛素刺激的DNA合成、GH诱导的IRS-4酪氨酸磷酸化和Stat5的核转位。IRS-4还与IRAS结合，当其过表达时，增强IRS-4依赖的胰岛素刺激PI3-激酶。IRS-4蛋白在心脏和骨骼肌组织中表现出有限的纤维类型特异性表达，尚未在任何小鼠或原代人组织中检测到。小鼠中缺乏IRS-4会导致生长、繁殖和葡萄糖稳态轻度缺陷，而IRS-4过表达会增加基础PI3-激酶活性和Akt磷酸化。IRS-4缺陷小鼠的缺陷可能是由于总体血糖浓度较低。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IRS-4 抗体 (RI-7) 参考文献:

  1. 小儿 T 细胞急性淋巴细胞白血病中的胰岛素受体底物 4 基因（IRS4）发生突变。  |  Karrman, K., et al. 2011. Br J Haematol. 155: 516-9. PMID: 21517825
  2. 在下丘脑神经元中，杏仁蛋白重复序列和含SOCS盒的蛋白4（Asb-4）与胰岛素受体底物4（IRS4）共定位，并介导IRS4降解。  |  Li, JY., et al. 2011. BMC Neurosci. 12: 95. PMID: 21955513
  3. IRS4 通过构成性 PI3K/AKT 通路超活化诱导乳腺肿瘤发生并产生对 HER2 靶向疗法的抗药性。  |  Ikink, GJ., et al. 2016. Nat Commun. 7: 13567. PMID: 27876799
  4. 下调 miR-493 可抑制 IRS4 的表达，从而促进黑色素瘤的增殖。  |  Cui, A., et al. 2017. Tumour Biol. 39: 1010428317701640. PMID: 28475006
  5. CK1γ2对IRS4的磷酸化促进其通过泛素/溶酶体途径被CHIP降解。  |  Li, X., et al. 2018. Theranostics. 8: 3643-3653. PMID: 30026872
  6. IRS4 通过激活 PI3K/Akt 和 Ras-MAPK 通路，促进非小细胞肺癌的进展，并赋予其对表皮生长因子受体-TKI 的抗性。  |  Hao, P., et al. 2021. Exp Cell Res. 403: 112615. PMID: 33894221
  7. 结合 eIF3I 触发的 PD-L1 通过调节 IRS4 促进糖尿病相关伤口愈合缺陷的改善  |  Kuai, L., et al. 2022. J Invest Dermatol. 142: 220-231.e8. PMID: 34293353
  8. FER 介导的 IRS4 磷酸化和 PIK3R2 招募促进了卵巢癌细胞中 AKT 的活化和肿瘤发生。  |  Zhang, Y., et al. 2022. Elife. 11: PMID: 35550247
  9. HDAC 抑制剂以 IRS4 为靶点，加强 AR 阳性三阴性乳腺癌的抗 AR 治疗。  |  He, Y., et al. 2024. Int J Oncol. 64: PMID: 38214343
  10. 伊维菌素通过抑制IRS4/PI3K/AKT信号通路抑制维莫非尼耐药黑色素瘤的生长  |  Guo, X., et al. 2024. J Nat Med. 78: 342-354. PMID: 38324123

订购信息

产品名称产品编号规格价格数量收藏夹

IRS-4 抗体 (RI-7)

sc-100854
100 µg/ml
$333.00

Which IRS4 epitope is recognized by the antibody? Is it N-terminal within the amino acids 3-342? Thank you.

Asked by: thegut
Thank you for your question. The exact epitope used to raise this monoclonal antibody has not been mapped so we are unable to provide this information. Please contact our Technical Service Department for further support. We are happy to help perform sequence analysis for you to answer any specific questions you have about the antibody reactivity.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30

For Western Blot, is it recommended to use denatured or non-denatured conditions with IRS-4 (RI-7): sc-100854 antibody?

Asked by: Trav11
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from Clean band in WB indicating a good antibodyClean band in WB indicating a good antibody to use especially in HeLa whole cell lysate. -SCBT QC
Date published: 2015-03-03
Rated 5 out of 5 by from Sizable band obtained in human IRSSizable band obtained in human IRS-4 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-01-19
Rated 4 out of 5 by from Good publishable data on Western blot onGood publishable data on Western blot on human endothelial cells. -SCBT Publication Review
Date published: 2015-01-16
Rated 5 out of 5 by from Publishable Western blot and IP data usingPublishable Western blot and IP data using human and mouse embryonic kidney epithelial 293T cells and NIH 3T3s -SCBT Publication Review
Date published: 2013-10-05
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IRS-4 抗体 (RI-7) is rated 4.5 out of 5 by 4.
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