Integrin α9/ITGA9 Double Nickase Plasmid (h): sc-402221-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin α9/ITGA9 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin α9/ITGA9 Double Nickase Plasmid (h) and Integrin α9/ITGA9 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITGA9. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin α9/ITGA9 Double Nickase Plasmid (h) | sc-402221-NIC | 20 µg | $410.00 | |||
Integrin α9/ITGA9 Double Nickase Plasmid (h2) | sc-402221-NIC-2 | 20 µg | $410.00 |
Human ITGA9 encodes integrin α9, an α subunit that heterodimerizes primarily with β1 to form a cell-surface receptor mediating adhesion to extracellular matrix ligands and guidance cues. Integrin α9/ITGA9 coordinates outside-in and inside-out signaling that influences cytoskeletal remodeling, focal adhesion turnover, and directed cell migration through pathways involving FAK/Src, PI3K–AKT, and MAPK signaling. By regulating cell–matrix interactions, ITGA9 contributes to processes such as epithelial and mesenchymal cell motility, tissue remodeling, and lymphatic and neural-associated responses. Dysregulated integrin signaling and altered ITGA9 expression have been investigated in contexts including inflammation, fibrosis, and tumor-associated invasion and metastasis, making it relevant for mechanistic studies of adhesion-dependent signaling.
Integrin α9/ITGA9 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ITGA9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ITGA9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ITGA9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ITGA9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.