Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Integrin α7/ITGA7 Double Nickase Plasmid (h): sc-401678-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin α7/ITGA7 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin α7/ITGA7 Double Nickase Plasmid (h) and Integrin α7/ITGA7 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITGA7. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin α7/ITGA7 Antibody (E-2): sc-515716
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin α7/ITGA7 Double Nickase Plasmid (h) | sc-401678-NIC | 20 µg | $410.00 | |||
Integrin α7/ITGA7 Double Nickase Plasmid (h2) | sc-401678-NIC-2 | 20 µg | $410.00 |
ITGA7 encodes integrin α7, a laminin-binding α subunit that pairs with β1 to form a major adhesion receptor in skeletal and cardiac muscle. This heterodimer links extracellular matrix cues to the actin cytoskeleton through focal adhesion and integrin signaling networks, coordinating cell adhesion, migration, mechanotransduction, and myofiber stability. Integrin α7–dependent signaling intersects with pathways regulating cytoskeletal dynamics and survival, including FAK/SRC and downstream MAPK/PI3K axis modulation. Altered ITGA7 expression or function has been associated with muscular dystrophy-related phenotypes, impaired muscle regeneration, and changes in tumor cell adhesion and invasion programs, making it relevant for studies of myogenesis and extracellular matrix remodeling.
Integrin α7/ITGA7 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ITGA7 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ITGA7. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ITGA7 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ITGA7-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.