Date published: 2025-9-10
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Influenza A NP 抗体 (9G8): sc-101352

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说明书
  • Influenza A NP 抗体 9G8 是小鼠单克隆 IgG2a, 在24篇文献中引用，提供 50 µg/500 µl
  • 针对重组Influenza A NP
  • 推荐用于 Influenza A virus 来源的nucleoprotein (NP) WB, IF 和 ELISA检测; 不会与 Influenza B or other respiratory viruses产生交联反应
  • 关于如何获取Influenza A NP (9G8): sc-101352的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是Influenza A NP Antibody (9G8) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与Influenza A NP Antibody (9G8) 打包提供（请参阅下面的订购信息）。

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描述
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研究信息

関連項目

额外Influenza抗体，包括Influenza A m1, Influenza A m2, Influenza A NP, Influenza A ns1, Influenza A Virus Hemagglutinin, Influenza B, Influenza B M1, Influenza B NA和Influenza B NP

Influenza A NP抗体（9G8）是一种IgG2a κ型小鼠单克隆Influenza A NP抗体（也称为Influenza A NP抗体或Influenza A核蛋白（NP）抗体），可通过蛋白质印迹（WB）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测来自Influenza A病毒的核蛋白（NP）。Influenza A NP抗体（9G8）以非偶联的抗Influenza A NP抗体形式提供。Influenza A病毒是一种负义、单链、分段的RNA病毒，以鸟类为主要宿主，但也可能感染多种哺乳动物。所有已知的亚型在鸟类中均为地方性流行。Influenza A亚型的分类基于病毒外膜糖蛋白血凝素（HA）和神经氨酸酶（NA）亚型的组合。Influenza A有16种不同的HA抗原（H1-H16）和9种不同的NA抗原（N1-N9）。感染宿主生物的程度由HA决定，HA与含有末端唾液酸残基的寡糖的细胞表面蛋白相互作用。Influenza A核蛋白（NP）与其RNA基因组相关，并存在于核糖核蛋白（RNP）的八个独立片段中，每个片段都必须存在才能成功复制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Influenza A NP 抗体 (9G8) 参考文献:

  1. 病毒进入过程中的受体结合和膜融合：流感血凝素。  |  Skehel, JJ. and Wiley, DC. 2000. Annu Rev Biochem. 69: 531-69. PMID: 10966468
  2. 流感病毒血凝素构象转变为融合活性状态的早期步骤：血凝素的稳定性和能量学。  |  Huang, Q., et al. 2003. Biochim Biophys Acta. 1614: 3-13. PMID: 12873761
  3. 流感病毒血凝素集中在脂筏微域中，以实现高效的病毒融合。  |  Takeda, M., et al. 2003. Proc Natl Acad Sci U S A. 100: 14610-7. PMID: 14561897
  4. 流感病毒血凝素三聚发夹形成的完成促进了融合孔的打开和扩大。  |  Borrego-Diaz, E., et al. 2003. Virology. 316: 234-44. PMID: 14644606
  5. 融合肽的寡聚化通过增强膜的不稳定性来促进膜融合。  |  Lau, WL., et al. 2004. Biophys J. 86: 272-84. PMID: 14695269
  6. 用表达甲型流感NP的重组痘苗病毒免疫小鼠，可保护其免受人类和禽流感病毒的实验性感染。  |  Altstein, AD., et al. 2006. Arch Virol. 151: 921-31. PMID: 16292596
  7. 流感病毒血凝素免疫识别的结构基础。  |  Wilson, IA. and Cox, NJ. 1990. Annu Rev Immunol. 8: 737-71. PMID: 2188678
  8. 一种新型抗病毒靶标结构，涉及甲型流感病毒核蛋白的RNA结合、二聚化和核出口功能。  |  Kakisaka, M., et al. 2015. PLoS Pathog. 11: e1005062. PMID: 26222066
  9. 在衰老的近交系和远交系小鼠中，使用粘膜佐剂N3和表达鞭毛蛋白的DNA质粒进行鼻腔全流感免疫接种，可显著延长对甲型流感病毒的粘膜和全身免疫，并增强保护作用。  |  Hinkula, J., et al. 2019. Vaccines (Basel). 7: PMID: 31315253
  10. 甲型流感病毒的致病性：血凝素的关键作用。  |  Webster, RG. and Rott, R. 1987. Cell. 50: 665-6. PMID: 3304656
  11. 流感血凝素野生型和突变型的表达：折叠在细胞内运输中的作用。  |  Gething, MJ., et al. 1986. Cell. 46: 939-50. PMID: 3757030
  12. 流感病毒血凝素的免疫原结构。  |  Green, N., et al. 1982. Cell. 28: 477-87. PMID: 6176330

订购信息

产品名称产品编号规格价格数量收藏夹

Influenza A NP 抗体 (9G8)

sc-101352
50 µg/500 µl
$333.00

Influenza A NP (9G8): m-IgG Fc BP-HRP 套装

sc-537308
50 µg Ab; 10 µg BP
$354.00

Influenza A NP (9G8): m-IgGκ BP-HRP 套装

sc-534543
50 µg Ab; 40 µg BP
$354.00

What Influenza A strain Nucleoprotein does it recognize? H1N1, H7N7, H5N1,...?

Asked by: JLOU
Thank you for your question. Nucleoprotein is highly conserved across different Influenza A strains, so Influenza A NP Antibody (9G8): sc-101352 will be able to detect the protein in H1N1, H7N7, H5N1 and others.
Answered by: Tech Support Europe
Date published: 2025-04-03

Hi, I would like to inquire if this antibody can be used for IP. Thanks

Asked by: anshikasharma
Thank you for your question. This antibody has not been tested for IP (Immunopreciptation) and so it is not a recommended application at this time.
Answered by: Technical Support
Date published: 2018-12-21

What is the total volume provided of this Influenza A NP (9G8) antibody?

Asked by: cjMara
Thank you for your question. This antibody is provided as 50 µg in 500 µl total volume.
Answered by: Technical Support 5
Date published: 2017-01-11

Do you provide a positive control for use with Influenza A NP (9G8): sc-101352?

Asked by: cjMara
No, we do not offer any positive controls for our Influenza A NP antibodies.
Answered by: Technical Support 5
Date published: 2017-01-11
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Rated 1 out of 5 by from Didn't work for WBThe antibodies didn't detect NP from Influenza H5N1 in WB, tried several dilutions and nothing. I used then polyclonal antibodies and they worked at the first try.
Date published: 2022-05-25
Rated 5 out of 5 by from Works great for IFGood immunofluorescence signal for influenza strain PR8 NP even at 1:1,000 dilution!
Date published: 2022-02-25
Rated 5 out of 5 by from Antibody works wellThe Influenza A NP Antibody (9G8): sc-101352 is specific to Influenza A NP and works well for detection via Western blot.
Date published: 2017-01-13
Rated 5 out of 5 by from Antibody was published for use in WB on humanAntibody was published for use in WB on human A549 cells - SCBT Publication Review
Date published: 2015-01-13
Rated 5 out of 5 by from Good publishable data in IF (pfa) using humanGood publishable data in IF (pfa) using human lung epithelial A549 cells -SCBT Publication Review
Date published: 2013-06-03
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Influenza A NP抗体 (9G8) is rated 4.2 out of 5 by 5.
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