IL-20Rα CRISPR/Cas9 KO Plasmid (h): sc-405034
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-20Rα CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IL-20Rα genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-20Rα Antibody (EE09): sc-80065
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-20Rα CRISPR/Cas9 KO Plasmid (h) | sc-405034 | 20 µg | $397.00 |
Overview
IL20RA encodes interleukin‑20 receptor alpha (IL‑20Rα), a type I cytokine receptor subunit that heterodimerizes with IL20RB to form functional receptors for IL‑19, IL‑20, and IL‑24. Ligand engagement activates JAK/STAT signaling, with prominent STAT3 and STAT1 phosphorylation, linking IL‑20 family cytokines to epithelial and keratinocyte programs that regulate barrier function, antimicrobial responses, and tissue remodeling. IL‑20Rα-mediated signaling intersects with inflammatory networks that shape cytokine and chemokine expression and can influence proliferation and differentiation states in responsive cell types. Dysregulation of IL‑20 family pathways has been associated with inflammatory skin phenotypes and broader immune-mediated processes, making IL20RA a useful node for mechanistic studies of cytokine-driven epithelial biology.
IL-20Rα CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IL20RA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IL20RA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IL20RA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IL-20Rα protein expression.
This CRISPR knockout system enables efficient generation of IL20RA-deficient cell models for investigation of IL-20Rα signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IL20RA exon(s) critical for IL-20Rα function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IL20RA genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IL-20Rα CRISPR/Cas9 KO Plasmid (h) and IL-20Rα CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IL20RA locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IL-20Rα HDR Plasmid (h) and IL-20Rα HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IL20RA homology arms to support homology-directed repair at defined IL20RA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.