IL-18Rβ CRISPR/Cas9 KO Plasmid (m): sc-421095
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-18Rβ CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IL-18Rβ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-18Rβ CRISPR/Cas9 KO Plasmid (m) | sc-421095 | 20 µg | $397.00 |
Overview
Il18rap encodes IL-18 receptor accessory protein (IL-18Rβ), an essential co-receptor that heterodimerizes with IL-18Rα to form a functional IL-18 receptor complex on immune cells. Upon IL-18 binding, IL-18Rβ helps recruit MyD88 and downstream IRAK/TRAF6 signaling, promoting NF-κB and MAPK pathway activation and induction of inflammatory cytokines such as IFN-γ. This axis influences Th1 polarization, NK-cell effector function, and myeloid activation, linking Il18rap to regulation of innate and adaptive immune responses. Altered IL-18/IL-18R signaling has been implicated in inflammatory and autoimmune processes and is frequently studied in contexts of infection, sterile inflammation, and tumor immunology models in mouse.
IL-18Rβ CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Il18rap gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Il18rap together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Il18rap open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IL-18Rβ protein expression.
This CRISPR knockout system enables efficient generation of Il18rap-deficient cell models for investigation of IL-18Rβ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Il18rap exon(s) critical for IL-18Rβ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Il18rap genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IL-18Rβ CRISPR/Cas9 KO Plasmid (m) and IL-18Rβ CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Il18rap locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IL-18Rβ HDR Plasmid (m) and IL-18Rβ HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Il18rap homology arms to support homology-directed repair at defined Il18rap target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.