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western blotting image from recommended NFKBIA/IkB alpha (H-4) monoclonal antibody replacement. Image 354367 — 这种多克隆抗体已经停产。请参阅我们推荐的NFKBIA/IkB alpha (H-4)单克隆抗体(显示样本数据)。

这种多克隆抗体已经停产。请参阅我们推荐的NFKBIA/IkB alpha (H-4)单克隆抗体(显示样本数据)。

IκB-α 抗体 (C-21): sc-371

4.8(30)

寫評論提問

I kappa B- alpha 抗体 C-21 是兔多克隆 IgG，提供 100 µg/ml; 或者山羊IgG; 200 µg/ml, sc-371-G
抗原表位位于human物种的IκB-α的C-terminus
下架的多克隆抗体

查看产品引用文献(907)

此外 I κ B 抗体包括 I kappa B-α, I kappa B-β 和 I κ B-ε

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor^® 是Molecular Probes Inc., OR., USA的商标

NFKBIA/IkB alpha (H-4): sc-1643 [用于替代IκB-α (C-21)的推荐单抗]

产品名称	产品编号	规格	价格	数量	收藏夹
NFKBIA/IkB alpha 抗体 (H-4)	sc-1643	200 µg/ml	$316.00
NFKBIA/IkB alpha (H-4): m-IgG Fc BP-HRP 套装	sc-528129	200 µg Ab; 10 µg BP	$354.00
NFKBIA/IkB alpha (H-4): m-IgGκ BP-HRP 套装	sc-520432	200 µg Ab, 40 µg BP	$354.00
NFKBIA/IkB alpha 抗体 (H-4) AC	sc-1643 AC	500 µg/ml, 25% agarose	$416.00
NFKBIA/IkB alpha 抗体 (H-4) HRP	sc-1643 HRP	200 µg/ml	$316.00
NFKBIA/IkB alpha 抗体 (H-4) FITC	sc-1643 FITC	200 µg/ml	$330.00
NFKBIA/IkB alpha 抗体 (H-4) PE	sc-1643 PE	200 µg/ml	$343.00
NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 488	sc-1643 AF488	200 µg/ml	$357.00
NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 546	sc-1643 AF546	200 µg/ml	$357.00
NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 594	sc-1643 AF594	200 µg/ml	$357.00
NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 647	sc-1643 AF647	200 µg/ml	$357.00
NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 680	sc-1643 AF680	200 µg/ml	$357.00
NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 790	sc-1643 AF790	200 µg/ml	$357.00
NFKBIA/IkB alpha 抗体 (H-4) TRITC	sc-1643 TRITC	200 µg/ml	$320.00

Hi, I've tried this antibody in WB using 30 ug of rat  liver cytoplasm lysate. I blocked nitrocellulose membrane with 10% skim milk for 1 h followed by overnight incubation with 1:100 primary antibody. Unfortunately I saw only a band at 90kDa.

Asked by: dany58

Thanks for your feedback. We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Please contact our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge. Please contact our Technical Service by phone or email or by Live Chat if you need support or additional information

Answered by: SCBT Heidelberg Support

Date published: 2018-11-27

hi, i keep getting two bands using this antibody. one band appears at around 37 kda and another one above 37 kda. could you explain what i can do to solve this problem? i'm using hacat (human keratinocyte cell line), 5% skim milk for blocking thank you

Asked by: shiba

Thank you for your question about our rabbit polyclonal antibody, sc-371: IkB-alpha (C-21). The expected molecular weight for IkB-alpha is approximately 37 kDa. This protein may also be phosphorylated which is one possible explanation for why you may also be detecting an additional slightly higher molecular weight band in your samples. I would recommend including additional controls in your experiment to confirm if the additional band above 37 kDa is also IkB-alpha, and a result of the binding of the specific primary antibody. One suggestion is to treat your lysates with Lambda Phosphatase: sc-200312 (https://www.scbt.com/scbt/product/lambda-phosphatase?requestFrom=search) to release phosphate groups and observe the difference in detection by Western blot. I would also recommend to perform a secondary antibody control blot (without primary antibody) to confirm that there is not any non-specific binding of your secondary antibody alone. Please contact our Technical Service Department for additional support in troubleshooting this antibody in your research application. https://www.scbt.com/scbt/resources/technical-service

Answered by: Technical Support

Date published: 2018-12-18

Rated 5 out of 5 by EBV girl from Good for western blotFor western blot, use at 1:1000 in 5% milk TBST, overnight incubation with rocking at 4 degrees C. Example of published result: PMID 29950426

Date published: 2020-01-09

Rated 5 out of 5 by NatasaV from IkB antibody in rat samplesIt is working very well in the rat samples without unspecific bands

Date published: 2019-08-02

Rated 5 out of 5 by sun2019 from quality of anti-IkBa antibodyThis antibody's quality is very good, and works very well in our hand.

Date published: 2019-01-15

Rated 4 out of 5 by KuanYH from Nice primary antibodyThe primary antibody is available for Western blotting in mice sample.

Date published: 2017-06-13

Rated 5 out of 5 by Eshu from Works really wellI have used this antibody for nearly five years and it worked really well with bone-marrow derived dendritic cell extracts of BALB/c and C57BL/6 mice. There were no non-specific bands and the band intensity for the specific bands was also very good.

Date published: 2017-06-06

Rated 4 out of 5 by Steventian from Excellent experience.I bought this antobody for my current research project involved in the IkB-alpha protein detection by western blot assay for the mice kidney samples, this antibody works very well!

Date published: 2017-02-12

Rated 5 out of 5 by Carme from Very clean immunoblots and high sensitivityThis is a great antibody for immunoblot analisis of endogenous IkBalpha. Very clean western blots and it can be diluted even 1:5000.

Date published: 2016-12-21

Rated 5 out of 5 by Jing H from Great result in WBGreat result in WB using human and rat lungs tissue.

Date published: 2016-11-04

IκB-α 抗体 (C-21) Reviews - page 2

IκB-α 抗体 (C-21) is rated 4.8 out of 5 by 30.

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IκB-α 抗体 (C-21): sc-371

NFKBIA/IkB alpha 抗体 (H-4)

NFKBIA/IkB alpha (H-4): m-IgG Fc BP-HRP 套装

NFKBIA/IkB alpha (H-4): m-IgGκ BP-HRP 套装

NFKBIA/IkB alpha 抗体 (H-4) AC

NFKBIA/IkB alpha 抗体 (H-4) HRP

NFKBIA/IkB alpha 抗体 (H-4) FITC

NFKBIA/IkB alpha 抗体 (H-4) PE

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor® 488

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor® 546

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor® 594

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor® 647

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor® 680

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor® 790

NFKBIA/IkB alpha 抗体 (H-4) TRITC

Hi, I've tried this antibody in WB using 30 ug of rat &nbsp;liver cytoplasm lysate. I blocked nitrocellulose membrane with 10% skim milk for 1 h followed by overnight incubation with 1:100 primary antibody. Unfortunately I saw only a band at 90kDa.

hi, i keep getting two bands using this antibody. one band appears at around 37 kda and another one above 37 kda. could you explain what i can do to solve this problem? i'm using hacat (human keratinocyte cell line), 5% skim milk for blocking thank you

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 488

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 546

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 594

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 647

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 680

NFKBIA/IkB alpha 抗体 (H-4) Alexa Fluor^® 790

Hi, I've tried this antibody in WB using 30 ug of rat liver cytoplasm lysate. I blocked nitrocellulose membrane with 10% skim milk for 1 h followed by overnight incubation with 1:100 primary antibody. Unfortunately I saw only a band at 90kDa.