IFITM1/2/3抗体 (F-12) 推荐用于 human 来源的IFITM1, IFITM2 and IFITM3 WB, IP, IF, IHC(P) 和 ELISA检测

IFITM1/2/3 抗体 (F-12): sc-374026

IFITM1/2/3抗体 (F-12) is rated 4.8 out of 5 by 4.
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说明书

    • 抗-IFITM1/2/3 抗体 F-12 是小鼠单克隆 IgG1 κ,IFITM1/2/3抗体, 在3篇文献中引用,规格为200 µg/ml
    • raised against amino acids 1-125 representing full length IFITM1 of human origin
    • IFITM1/2/3 抗体 (F-12) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 IFITM1, IFITM2 and IFITM3
    • 抗-IFITM1/2/3 抗体 (F-12) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取IFITM1/2/3 (F-12): sc-374026免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for IFITM1/2/3 Antibody (F-12) for WB and IHC(P) applications. This reagent is now offered in a bundle with IFITM1/2/3 Antibody (F-12) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

I am using IFITM1/2/3 (F-12): sc-374026 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenni
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good for western blot! It detected endogenous IFIT3 in IFN-alpha-treated HeLa cells (1:500 dilution in 5% milk in TBS-0.1% Tween). Recommended!
Date published: 2017-11-01
Rated 5 out of 5 by from good antibody The results of Western Blot analysis were very good, the baseband was clear and significant, and the effect of IFITM1/2/3 expression in NIH/3T3 cell lysate was no problem.
Date published: 2017-06-01
Rated 5 out of 5 by from Produced great immunoperoxidase cytoplasmic Produced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human appendix tissue. -SCBT QC
Date published: 2015-04-04
Rated 5 out of 5 by from Produced positive Western Blot data of IFITM1 Produced positive Western Blot data of IFITM1 expression in non-transfected and human IFITM1 transfected 293 whole cell lysates. -SCBT QC
Date published: 2014-07-11
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