ICAM-1/CD54 Antibody (6.5B5): sc-18853

ICAM-1 Antibody (6.5B5) is a mouse monoclonal IgG1 kappa light chain antibody raised against TNF-activated human umbilical vein endothelial cells, demonstrating reactivity with mouse, rat, and human species. Anti-ICAM-1 antibody (6.5B5) is suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). ICAM-1, also known as CD54 or intercellular adhesion molecule-1, is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. Structurally, ICAM-1 consists of five extracellular immunoglobulin-like domains, a single transmembrane domain, and a short cytoplasmic tail. These multiple immunoglobulin-like domains are crucial for binding to leukocyte integrins such as LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18), facilitating cell-cell adhesion during immune responses. This molecular structure enables precise interactions required for leukocyte transmigration across the endothelium during inflammation. Alterations in ICAM-1 structure or expression can impact immune surveillance and contribute to various pathological conditions, including autoimmune diseases and cancer metastasis. ICAM-1 (6.5B5) monoclonal antibody serves as a valuable tool for studying cell adhesion, signaling, and immune regulation in immunology and cell biology research.

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ICAM-1/CD54 Antibody (6.5B5) References:

1. Expression and role of icam-1 in the occurrence and development of hepatocellular carcinoma.  |  Zhu, XW. and Gong, JP. 2013. Asian Pac J Cancer Prev. 14: 1579-83. PMID: 23679239
2. Autophagy facilitates cytokine-induced ICAM-1 expression.  |  Wang, CY., et al. 2014. Innate Immun. 20: 200-13. PMID: 23751820
3. ICAM-1-Related Noncoding RNA in Cancer Stem Cells Maintains ICAM-1 Expression in Hepatocellular Carcinoma.  |  Guo, W., et al. 2016. Clin Cancer Res. 22: 2041-50. PMID: 26667486
4. Essential role of ICAM-1 in aldosterone-induced atherosclerosis.  |  Marzolla, V., et al. 2017. Int J Cardiol. 232: 233-242. PMID: 28089144
5. Genetic and Biological Effects of ICAM-1 E469K Polymorphism in Diabetic Kidney Disease.  |  Zhang, X., et al. 2020. J Diabetes Res. 2020: 8305460. PMID: 32626783
6. Macrophage ICAM-1 functions as a regulator of phagocytosis in LPS induced endotoxemia.  |  Zhong, H., et al. 2021. Inflamm Res. 70: 193-203. PMID: 33474594
7. Hspa8 and ICAM-1 as damage-induced mediators of γδ T cell activation.  |  Johnson, MD., et al. 2022. J Leukoc Biol. 111: 135-145. PMID: 33847413
8. Cordycerebroside A inhibits ICAM-1-dependent M1 monocyte adhesion to osteoarthritis synovial fibroblasts.  |  Lee, KT., et al. 2022. J Food Biochem. 46: e14108. PMID: 35165902
9. NMT1 sustains ICAM-1 to modulate adhesion and migration of tumor cells.  |  Wang, H., et al. 2023. Cell Signal. 109: 110739. PMID: 37269961
10. ICAM-1 on epithelial cells in allergic subjects: a hallmark of allergic inflammation.  |  Canonica, GW., et al. 1995. Int Arch Allergy Immunol. 107: 99-102. PMID: 7613226

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ICAM-1/CD54 Antibody (6.5B5) has 38 citations in a variety of scientific publications.

Primary antibodies like Anti-ICAM-1/CD54 Antibody (6.5B5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ICAM-1/CD54 Antibody (6.5B5) Specifications

Alternate Product Name: CD54

Product Clone Name: 6.5B5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: ICAM-1

Epitope Species: human

Product Reactivity/Detection: ICAM-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 38 citations in various scientific publications

Available Conjugates/Forms for ICAM-1/CD54 Antibody (6.5B5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred ICAM-1/CD54 antibody is ICAM-1/CD54 Antibody (G-5): sc-8439.