ICAM-1/CD54 Antibody (5K16): sc-71303
- ICAM-1/CD54 Antibody (5K16) is a rat monoclonal IgG2b, cited in 4 publications, provided at 200 µg/ml
- recommended for detection of ICAM-1 of mouse origin by WB, IP, IF, IHC(P) and FCM
- available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
- See ICAM-1/CD54 (G-5): sc-8439 for ICAM-1/CD54 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ICAM-1/CD54 Antibody (5K16). This work is in progress.
ICAM-1 Antibody (5K16) is a rat monoclonal IgG2b antibody demonstrating reactivity with mouse species, suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). ICAM-1, also known as CD54 or intercellular adhesion molecule-1, is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. ICAM-1 plays a crucial role in immune responses by mediating cell-cell adhesion through interactions with leukocyte integrins like LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). These interactions facilitate the adhesion and transmigration of leukocytes across the endothelium during inflammation, which is essential for immune surveillance and host defense mechanisms. ICAM-1 expression is upregulated in response to pro-inflammatory cytokines, making ICAM-1 a key molecule in inflammatory processes. Dysregulation of ICAM-1 is associated with various pathological conditions, including autoimmune diseases, cardiovascular disorders, and cancer metastasis. Understanding ICAM-1 function is important for developing therapeutic strategies targeting inflammation and immune responses. Anti-ICAM-1 antibody (5K16) provides a valuable tool for studying ICAM-1 expression, localization, and function in physiological and pathological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ICAM-1/CD54 Antibody (5K16) References:
- Value of ICAM-1 expression and soluble ICAM-1 level as a marker of activity in sarcoidosis. | Kim, DS., et al. 1999. Chest. 115: 1059-65. PMID: 10208208
- ICAM-1 and CD11b inhibition worsen outcome in rats with E. coli pneumonia. | Zeni, F., et al. 1999. J Appl Physiol (1985). 87: 299-307. PMID: 10409588
- The proinflammatory phenotype of senescent cells: the p53-mediated ICAM-1 expression. | Kletsas, D., et al. 2004. Ann N Y Acad Sci. 1019: 330-2. PMID: 15247038
- Downregulation of ICAM-1 and VCAM-1 expression in endothelial cells treated by photodynamic therapy. | Volanti, C., et al. 2004. Oncogene. 23: 8649-58. PMID: 15467759
- ICAM-1 inhibits the homocluster formation of MHC-I in colon carcinoma cells. | Nagy, P., et al. 2006. Biochem Biophys Res Commun. 347: 758-63. PMID: 16844085
- IL-6 promotes ICAM-1 expression and cell motility in human osteosarcoma. | Lin, YM., et al. 2013. Cancer Lett. 328: 135-43. PMID: 22939995
- Identification of a high-mannose ICAM-1 glycoform: effects of ICAM-1 hypoglycosylation on monocyte adhesion and outside in signaling. | Scott, DW., et al. 2013. Am J Physiol Cell Physiol. 305: C228-37. PMID: 23703526
- Emerging Functions of ICAM-1 in Macrophage Efferocytosis and Wound Healing. | Dalal, PJ. and Sumagin, R. 2020. J Cell Immunol. 2: 250-253. PMID: 33426539
- Acute Myeloid Leukemia Epigenetic Immune Escape From Nature Killer Cells by ICAM-1. | Xiao, Y., et al. 2021. Front Oncol. 11: 751834. PMID: 34722306
- Involvement of adhesion molecules LFA-1 and ICAM-1 in osteoclast development. | Kurachi, T., et al. 1993. Biochim Biophys Acta. 1178: 259-66. PMID: 7779165
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ICAM-1/CD54 Antibody (5K16) | sc-71303 | 200 µg/ml | $322.00 | |||
ICAM-1/CD54 Antibody (5K16) FITC | sc-71303 FITC | 200 µg/ml | $336.00 | |||
ICAM-1/CD54 Antibody (5K16) PE | sc-71303 PE | 200 µg/ml | $349.00 |