Hygromycin B solution CAS: 31282-04-9
MF: C20H37N3O13
MW: 527.52
An antibiotic often used for selection of cells transfected with E. coli hygromycin resistance gene..

Hygromycin B solution (CAS 31282-04-9)

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同义词: Hygrovetine; D-streptamine
应用; An antibiotic often used for selection of cells transfected with E. coli hygromycin resistance gene.
CAS号码: 31282-04-9
纯度: >90%
分子量: 527.52
分子式: C20H37N3O13
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
* 参考分析证明 大量特定数据 (包括水 含量).
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Hygromycin B is an aminoglycoside antibiotic substance naturally produced by Streptomyces hygroscopicus, which also shows anthelmintic activity. Hygromycin B has been shown to effectively inhibit translocation of mRNA and tRNAs on the ribosome in both bacteria and eukaryotes and also has a subtle effect on decoding fidelity as it slightly increases aminoacyl tRNA (A-site tRNA) affinity. Suitable for the selection of cells transfected with Escherichia coli hygromycin resistance gene (hyg or hph), as well as for the maintenance of the Hygromycin phenotype of resistant cells. Consistent with its more specific effect on translocation, hygromycin B exhibits monophasic inhibition of polypeptide synthesis, suggesting that it binds to a single site on the ribosome.


参考文献

1. González, A., et al. 1978. Biochim. Biophys. Acta. 521: 459-469. PMID: 367435
2. Rao, R.N., et al. 1983. Antimicrob. Agents Chemother. 24: 689-695. PMID: 6318654
3. Cullen, D., et al. 1987. Gene. 57: 21-26. PMID: 3322945
4. Perlin, D.S., et al. 1988. J. Biol. Chem. 263: 18118-18122. PMID: 3056938
5. Kronstad, J.W., et al. 1989. Gene. 79: 97-106. PMID: 2673937
6. Perlin, D.S., et al. 1989. J. Biol. Chem. 264: 21857-21864. PMID: 2532214
7. Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9: 1965-1968. PMID: 2546056
8. Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34: 1915-1920. PMID: 1963287
9. Bilang, R., et al. 1991. Gene. 100: 247-250. PMID: 1647356
10. Dean, N., 1995. Proc. Natl. Acad. Sci. U.S.A. 92: 1287-1291. PMID: 7877969

用法 :
• This product is provided as 20 mL of a sterile filtered solution in PBS.


• Appearance: Solution color can vary from light yellow to a dark brown or caramel color. Concentrated Hygromycin solution tends to have a darker appearance.


• The working concentration for the purpose of selection varies with cell type, media, growth conditions and cell metabolic rate. A titration experiment for each cell type should be performed to determine the amount of Hygromycin B needed to kill non-transfected cells.


• Recommended concentrations for the selection of resistant cells is 25-1000 μg/ml. Commonly used concentrations for selection are 200 μg/ml for mammalian cells, 20-200 μg/ml for plant cells & bacteria cells and 200-1000 μg/ml for fungi. Your optimum concentration should be tested experimentally.


• Control cells should die within 5-7 days after addition of the antibiotic allowing colonies of resistant cells to form by 10–14 days.
规划 :
50 mg/ml in PBS
外观 :
Solution
物理状态 :
Liquid
来源于 :
Streptomyces hygroscopicus
溶解度 :
Soluble in water, methanol, buffer solution, and ethanol.
保存 :
Store at 4° C
仅供科研使用。不可用于诊断或治疗。
RTECS :
WK2130000
PubChem CID :
35766
默克索引 :
14: 4852
MDL 号码 :
MFCD06795479
EC号码 :
250-545-5

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PMID: # 30829571  Vallardi, G.|Allan, LA.|Crozier, L.|Saurin, AT.| et al. 2019. Elife. 8:

PMID: # 30838870  Wu, M.|Feng, Y.|Ye, GX.|Han, YC.|Wang, SS.|Ni, HF.|Wang, FM.|Gao, M.|Lv, LL.|Liu, BC.| et al. 2019. Am. J. Physiol. Renal Physiol. 316: F1006-F1015.

PMID: # 30881116  Chiang, KC.|Huang, ST.|Wu, RC.|Huang, SC.|Yeh, TS.|Chen, MH.|Hsu, JT.|Chen, LW.|Kuo, SF.|Chueh, HY.|Juang, HH.|Hung, SI.|Yeh, CN.|Pang, JS.| et al. 2019. Cancer Manag Res. 11: 1893-1905.

PMID: # 30034634  Barone, G.|Arora, A.|Ganesh, A.|Abdel-Fatah, T.|Moseley, P.|Ali, R.|Chan, SY.|Savva, C.|Schiavone, K.|Carmell, N.|Myers, KN.|Rakha, EA.|Madhusudan, S.|Collis, SJ.| et al. 2018. Oncotarget. 9: 29508-29524.

PMID: # 28674183  Paclíková, P. et al. 2017. Mol. Cell. Biol.

PMID: # 28870912  Pan, PJ. et al. 2017. Anticancer Res. 37: 4911-4918.

PMID: # 25271153  Kannike, K. et al. 2014. J. Biol. Chem. 289: 32845-57.

PMID: # 24606125  Eigler, T. et al. 2014. Mol. Endocrinol. 28: 554-64.

PMID: # 19468300  Wolfe, BA. et al. 2009. PLoS Biol. 7: -.

PMID: # 17362967  Laurentino, EC. et al. 2007. Int. J. Parasitol. 37: 735-742.

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