Date published: 2025-9-6
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Huntingtin Antibody (3E10) - Western Blotting - Image 362888
Huntingtin 抗体 (3E10): sc-47757. HeLa (A) 和 K-562 (B) 全细胞裂解液中 Huntingtin 表达的 Western 印迹分析.
Huntingtin 抗体 (3E10): sc-47757. HeLa (A) 和 K-562 (B) 全细胞裂解液中 Huntingtin 表达的 Western 印迹分析.

Huntingtin 抗体 (3E10): sc-47757

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说明书
  • Huntingtin 抗体 3E10 是小鼠单克隆 IgG1 κ，Huntingtin抗体, 在5篇文献中引用，规格为200 µg/ml
  • 免疫mouse物种的Huntingtin的氨基酸997-1276
  • Huntingtin 抗体 (3E10) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 an epitope corresponding to the HDA region (amino acids 1171-1177) of Huntingin
  • 抗Huntingtin抗体（3E10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Huntingtin (3E10): sc-47757的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Huntingtin 抗体 (3E10)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

亨廷顿抗体（3E10）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）检测小鼠、大鼠和人类亨廷顿蛋白（HTT）。抗HTT抗体（3E10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。HTT（3E10）单克隆抗体在研究神经元功能、细胞内运输、转录调控和突触功能方面发挥着至关重要的作用。 HTT的正常功能对于维持神经元健康至关重要，而HTT的突变，特别是导致多聚谷氨酰胺区域扩增的突变，与亨廷顿病有关。 多聚谷氨酰胺异常扩增会导致功能毒性增强，通过可能涉及与其他细胞蛋白相互作用改变的机制导致神经变性。值得注意的是，HTT与几种关键蛋白相互作用，包括HAP1、磷酸甘油醛脱氢酶（GAPDH）和HIP1，这些蛋白与各种细胞过程有关。了解这些相互作用对于阐明亨廷顿病的病理生理学和制定潜在的治疗策略至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Huntingtin 抗体 (3E10) 参考文献:

  1. 大脑中富含的亨廷汀相关蛋白对病理学的影响。  |  Li, XJ., et al. 1995. Nature. 378: 398-402. PMID: 7477378
  2. 亨廷顿氏病的遗传学和分子生物学。  |  Albin, RL. and Tagle, DA. 1995. Trends Neurosci. 18: 11-4. PMID: 7535483
  3. 亨廷顿氏病基因的结构和表达：证据表明，CAG 重复序列的扩大不会导致简单的失活。  |  Ambrose, CM., et al. 1994. Somat Cell Mol Genet. 20: 27-38. PMID: 8197474
  4. 亨廷顿氏病染色体上含有三核苷酸重复的新型基因，该基因在亨廷顿氏病染色体上扩增且不稳定。亨廷顿氏病合作研究小组。  |  . 1993. Cell. 72: 971-83. PMID: 8458085
  5. 亨廷汀蛋白和 DRPLA 蛋白选择性地与 GAPDH 酶相互作用。  |  Burke, JR., et al. 1996. Nat Med. 2: 347-50. PMID: 8612237
  6. HIP1是S. cerevisiae Sla2p的人类同源物，在大脑中与膜缔合作用的亨廷蛋白相互作用。  |  Kalchman, MA., et al. 1997. Nat Genet. 16: 44-53. PMID: 9140394
  7. 亨廷顿氏症  |  Gusella, JF., et al. 1996. Cold Spring Harb Symp Quant Biol. 61: 615-26. PMID: 9246488
  8. 亨廷汀蛋白在细胞核内诱导细胞凋亡，但死亡与核内包涵体的形成无关。  |  Saudou, F., et al. 1998. Cell. 95: 55-66. PMID: 9778247

订购信息

产品名称产品编号规格价格数量收藏夹

Huntingtin 抗体 (3E10)

sc-47757
200 µg/ml
$316.00

Huntingtin 抗体 (3E10) AC

sc-47757 AC
500 µg/ml, 25% agarose
$416.00

Huntingtin 抗体 (3E10) HRP

sc-47757 HRP
200 µg/ml
$316.00

Huntingtin 抗体 (3E10) FITC

sc-47757 FITC
200 µg/ml
$330.00

Huntingtin 抗体 (3E10) PE

sc-47757 PE
200 µg/ml
$343.00

Huntingtin 抗体 (3E10) Alexa Fluor® 488

sc-47757 AF488
200 µg/ml
$357.00

Huntingtin 抗体 (3E10) Alexa Fluor® 546

sc-47757 AF546
200 µg/ml
$357.00

Huntingtin 抗体 (3E10) Alexa Fluor® 594

sc-47757 AF594
200 µg/ml
$357.00

Huntingtin 抗体 (3E10) Alexa Fluor® 647

sc-47757 AF647
200 µg/ml
$357.00

Huntingtin 抗体 (3E10) Alexa Fluor® 680

sc-47757 AF680
200 µg/ml
$357.00

Huntingtin 抗体 (3E10) Alexa Fluor® 790

sc-47757 AF790
200 µg/ml
$357.00

The Huntingtin Antibody (3E10): sc-47757 links to the N-Terminal of the protein?

Asked by: Carina
Thank you for your question. Huntingtin Antibody (3E10): sc-47757 was raised against amino acids 997-1276 of Huntingtin of mouse origin.
Answered by: Tech Support Europe
Date published: 2023-12-14

Can I get a quote about Huntingtin Antibody (3E10): sc-47757?

Asked by: zcczcw
Thank you for your question. For accurate availability, shipping and pricing information regarding our products, or to receive a quote, please contact Customer Service.
Answered by: Tech Service
Date published: 2020-03-12

I am interested in the FREE 10 µg sample of Huntingtin (3E10): sc-47757. I want yo know what is the procedure for this request

Asked by: MrMauricio
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

I am using Huntingtin (3E10): sc-47757 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good!This produced clean and specific signals in WB (5% milk, 1:200)
Date published: 2018-11-13
Rated 4 out of 5 by from Good AntibodyI tested this antibody on mouse brain lysate. I detected two major protein bands in WB (1:200 dilution). One protein band is around 400 kDa and another one is 130 kDa protein band.
Date published: 2018-07-09
Rated 5 out of 5 by from Good for WBI have tested by WB, 1:1000 (blocking milk 5% and primary Ab dilution in BSA 3% ON) and it worked very well for both endogenous protein and recombinant HTT.
Date published: 2017-11-07
Rated 4 out of 5 by from Works wellWe have this antibody in our lab and found it works well.
Date published: 2017-04-05
Rated 5 out of 5 by from western blottingUsed for western blotting for mouse liver tissue analysis , the antibody can work well in 1:500, strong signal.
Date published: 2017-03-18
Rated 4 out of 5 by from Potent and fairly specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium detection of the ~350 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, except for medium immunoreactivity with a ~100 kDa protein.
Date published: 2016-11-10
Rated 4 out of 5 by from Wonderful immunoperoxidase cytoplasmic stainingWonderful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human cerebral cortex tissue. -SCBT QC
Date published: 2015-04-24
Rated 4 out of 5 by from Single strong band at correct mw in JurkatSingle strong band at correct mw in Jurkat whole cell lysate. -SCBT QC
Date published: 2013-05-22
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Huntingtin 抗体 (3E10) is rated 4.4 out of 5 by 8.
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