HSP 60 Antibody (F-9): sc-376261

HSP 60 Antibody (F-9) is a mouse monoclonal IgG2a antibody that detects HSP 60 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). HSP 60, a member of the heat shock protein family, plays a crucial role as a molecular chaperone, primarily located in the mitochondria, where HSP 60 assists in the proper folding and assembly of newly synthesized proteins destined for the mitochondrial matrix. This localization is vital because mitochondrial dysfunction can lead to a range of diseases, including neurodegenerative disorders and metabolic syndromes. By ensuring that proteins are correctly folded and functional, HSP 60 helps maintain cellular homeostasis and protects against stress-induced damage. Additionally, HSP 60 has been implicated in immune responses, acting as a cell surface marker for γ/δ T cell recognition, which highlights HSP 60′s importance not only in cellular function but also in the immune system. Anti-HSP 60 antibody (F-9) is an essential tool for researchers studying mitochondrial biology, protein folding mechanisms, and immune interactions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HSP 60 Antibody (F-9) References:

1. Identification of HSP-60 as the specific antigen of IgM produced by BRG-lymphoma cells.  |  Musante, L., et al. 1999. Electrophoresis. 20: 1092-7. PMID: 10344290
2. Human HSP 60 peptide responsive T cell lines are similarly present in both Behçet's disease patients and healthy controls.  |  Saruhan-Direskeneli, G., et al. 2001. Immunol Lett. 79: 203-8. PMID: 11600199
3. Seminal antibodies to human 60kd heat shock protein (HSP 60) in male partners of subfertile couples.  |  Eggert-Kruse, W., et al. 2002. Hum Reprod. 17: 726-35. PMID: 11870127
4. Immunohistochemical analysis of TNF-alpha and HSP-60 in women with tubal factor infertility associated with Chlamydia trachomatis.  |  Zhao, H. and Li, H. 2004. J Huazhong Univ Sci Technolog Med Sci. 24: 630-2. PMID: 15791863
5. Enhanced characterization of serum autoantibody reactivity following HSP 60 immunization in a rat model of experimental autoimmune glaucoma.  |  Joachim, SC., et al. 2010. Curr Eye Res. 35: 900-8. PMID: 20858111
6. Role of anti-HSP 60/65 antibodies in atherogenesis in patients with type 2 diabetes and lower limb ischemia.  |  Rabczynski, M., et al. 2012. J Physiol Pharmacol. 63: 691-6. PMID: 23388486
7. ortho-Carboranylphenoxyacetanilides as inhibitors of hypoxia-inducible factor (HIF)-1 transcriptional activity and heat shock protein (HSP) 60 chaperon activity.  |  Li, G., et al. 2015. Bioorg Med Chem Lett. 25: 2624-8. PMID: 25981686
8. Himatanthus drasticus (Apocynaceae) latex reduces oxidative stress and modulates CD4+, CD8+, FoxP3+ and HSP-60+ expressions in Sarcoma 180-bearing mice.  |  Santos, GJL., et al. 2018. J Ethnopharmacol. 220: 159-168. PMID: 29079220
9. Characteristics of enzyme-linked immunosorbent assay for detection of IgG antibodies specific to Сhlamydia trachomatis heat shock protein (HSP-60).  |  Galkin, OY., et al. 2017. Ukr Biochem J. 89: 22-30. PMID: 29236386
10. Correlation of the survival of ovarian cancer patients with mRNA expression of the 60-kD heat-shock protein HSP-60.  |  Kimura, E., et al. 1993. J Clin Oncol. 11: 891-8. PMID: 8098058

The preferred HSP 60 antibody is HSP 60 Antibody (H-1): sc-13115.