Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HSP 105 Double Nickase Plasmid (h): sc-402155-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSP 105 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HSP 105 Double Nickase Plasmid (h) and HSP 105 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HSPH1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSP 105 Antibody (B-7): sc-74550
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSP 105 Double Nickase Plasmid (h) | sc-402155-NIC | 20 µg | $410.00 | |||
HSP 105 Double Nickase Plasmid (h2) | sc-402155-NIC-2 | 20 µg | $410.00 |
HSPH1 encodes the heat shock protein HSP 105, a high-molecular-weight molecular chaperone induced by proteotoxic stress that helps prevent protein aggregation and supports refolding of misfolded clients in the cytosol. HSP 105 cooperates with HSP70/HSP40 chaperone systems and contributes to proteostasis networks linked to the heat shock response, ubiquitin–proteasome function, and cellular recovery after thermal or oxidative stress. By stabilizing proteins under stress, HSPH1 influences apoptosis resistance, cell cycle progression, and stress-adaptive signaling programs. Dysregulated chaperone activity and elevated HSPH1 expression have been associated with contexts of malignant transformation and other proteostasis-imbalanced states, making it relevant for mechanistic studies of stress tolerance and protein quality control.
HSP 105 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HSPH1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HSPH1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HSPH1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HSPH1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.