HR CRISPR/Cas9 KO Plasmid (m): sc-420945
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HR CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HR genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HR Antibody (A-11): sc-514686
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HR CRISPR/Cas9 KO Plasmid (m) | sc-420945 | 20 µg | $397.00 |
Overview
Mouse Hr encodes the hairless (HR) nuclear protein, a transcriptional corepressor that modulates gene expression programs downstream of nuclear hormone receptors, including thyroid hormone and retinoic acid receptors. HR influences epidermal differentiation and hair follicle cycling by coordinating chromatin-associated repression in skin and adnexal tissues. Disruption of Hr function perturbs follicular morphogenesis and keratinocyte homeostasis, making it a widely used locus for studying pathways that govern hair growth and skin barrier biology. Hr is therefore relevant to experimental models of alopecia-like phenotypes and broader investigations of transcriptional control in cutaneous development.
HR CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hr gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hr together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hr open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HR protein expression.
This CRISPR knockout system enables efficient generation of Hr-deficient cell models for investigation of HR signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hr exon(s) critical for HR function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hr genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HR CRISPR/Cas9 KO Plasmid (m) and HR CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hr locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HR HDR Plasmid (m) and HR HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hr homology arms to support homology-directed repair at defined Hr target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.