Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HPK1 CRISPR/Cas9 KO Plasmid (h): sc-402367
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HPK1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HPK1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HPK1 Antibody (G-9): sc-374183
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HPK1 CRISPR/Cas9 KO Plasmid (h) | sc-402367 | 20 µg | $397.00 |
Overview
MAP4K1 encodes hematopoietic progenitor kinase 1 (HPK1), a Ste20 family serine/threonine kinase predominantly expressed in immune cells and positioned upstream of MAPK signaling. HPK1 functions as a negative regulator of antigen receptor signaling by modulating proximal adaptor complexes and shaping downstream JNK/p38 activation, thereby influencing T cell receptor and B cell receptor signaling outputs. Through these roles, HPK1 impacts lymphocyte activation thresholds, cytokine production, and immune synapse dynamics. Dysregulated MAP4K1/HPK1 activity has been associated with altered immune homeostasis and has been investigated in contexts of immune-mediated inflammation and tumor immunology as a modulator of anti-tumor immune responses.
HPK1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MAP4K1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MAP4K1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MAP4K1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HPK1 protein expression.
This CRISPR knockout system enables efficient generation of MAP4K1-deficient cell models for investigation of HPK1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MAP4K1 exon(s) critical for HPK1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MAP4K1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HPK1 CRISPR/Cas9 KO Plasmid (h) and HPK1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MAP4K1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HPK1 HDR Plasmid (h) and HPK1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MAP4K1 homology arms to support homology-directed repair at defined MAP4K1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.