Date published: 2025-9-7
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HoxA9 Antibody (HOX5I043) - Western Blotting - Image 368226
HoxA9 抗体 (HOX5I043): sc-81291. Hep G2 (A) 和 SW480 (B) 全细胞裂解液以及小鼠脊髓 (C) 和大鼠脊髓 (D) 组织提取物中 HoxA9 表达的 Western 印迹分析.
HoxA9 Antibody (HOX5I043) - Western Blotting - Image 368226
HoxA9 抗体 (HOX5I043): sc-81291. Hep G2 (A) 和 SW480 (B) 全细胞裂解液以及小鼠脊髓 (C) 和大鼠脊髓 (D) 组织提取物中 HoxA9 表达的 Western 印迹分析.
HoxA9 Antibody (HOX5I043) - Western Blotting - Image 127012
HoxA9 抗体 (HOX5I043): sc-81291. 未转染: sc-117752 (A) 和转染人 HoxA9: sc-174099 (B) 293T 全细胞裂解液中 HoxA9 表达的 Western 印迹分析.
HoxA9 Antibody (HOX5I043) - Western Blotting - Image 16855
HoxA9 抗体 (HOX5I043): sc-81291 人重组 HoxA9 融合蛋白的 Western 印迹分析.
HoxA9 抗体 (HOX5I043): sc-81291. Hep G2 (A) 和 SW480 (B) 全细胞裂解液以及小鼠脊髓 (C) 和大鼠脊髓 (D) 组织提取物中 HoxA9 表达的 Western 印迹分析.

HoxA9 抗体 (HOX5I043): sc-81291

3.8(4)
寫評論提問

说明书
  • HoxA9 抗体 HOX5I043 是小鼠单克隆 IgG1 κ，HoxA9抗体, 在10篇文献中引用，规格为100 µg/ml
  • 针对与HoxA9的human起源的C-terminal区域相对应的重组蛋白
  • 推荐用于 mouse, rat 和 human 来源的HoxA9 WB 和 IP检测
  • 关于如何获取HoxA9 (HOX5I043): sc-81291的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是HoxA9 Antibody (HOX5I043) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与HoxA9 Antibody (HOX5I043) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Hox抗体，包括Hox11, HoxA1, HoxA10, HoxA11, HoxA3, HoxA4, HoxA5, HoxA7, HoxA9, HoxB13, HoxB4, HoxB5, HoxB6, HoxB7, HoxB8和HoxB9

HoxA9抗体（HOX5I043）是一种IgG1 κ小鼠单克隆HoxA9抗体，能够通过蛋白质印迹法（WB）和免疫沉淀法（IP）检测小鼠、大鼠和人类来源的HoxA9蛋白。HoxA9抗体（HOX5I043）为非偶联抗HoxA9抗体。HOX同源盒基因编码在胚胎发育中起作用的蛋白质。HOXA9基因编码一种I类同源结构域蛋白，该蛋白在正常成年和胎儿胸腺组织中表达，并可能在调节胸腺细胞早期分化中发挥作用。HOXA9同源结构域蛋白与Meis1和Pbx1协同结合共识DNA序列。此外，HOXA9蛋白与MEIS1和Pbx 1蛋白一起涉及小鼠和人类的白血病转化。此外，在同基因小鼠的原代骨髓细胞中过表达HoxA9和Meis1可诱导生长因子依赖性急性髓系白血病（AML）。在人类AML和慢性髓系白血病（CML）患者中已证明存在t(7;11)(p15;p15)的染色体易位，导致融合基因NUP98-HOXA9的产生。移植了表达NUP98-NOXA9的骨髓细胞的小鼠会患上骨髓增生性疾病（MPD），最终会恶化为AML。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HoxA9 抗体 (HOX5I043) 参考文献:

  1. 造血干细胞中 NUP98-HOXA9 的表达可诱导小鼠患慢性和急性髓性白血病。  |  Kroon, E., et al. 2001. EMBO J. 20: 350-61. PMID: 11157742
  2. 单转位和双嵌合转录本：在染色体易位 t(7;11)(p15;p15)出现 HOXA11 或 HOXA13 断裂的骨髓性白血病中检测 NUP98-HOXA9。  |  Fujino, T., et al. 2002. Blood. 99: 1428-33. PMID: 11830496
  3. 人类髓性白血病中染色体易位 t(7;11)(p15;p15)导致核蛋白基因 NUP98 与 HOXA9 融合。  |  Nakamura, T., et al. 1996. Nat Genet. 12: 154-8. PMID: 8563753
  4. 在人类子宫内膜植入时，HOXA10会在性类固醇的作用下表达。  |  Taylor, HS., et al. 1998. J Clin Invest. 101: 1379-84. PMID: 9525980
  5. Hoxa9 通过与 Meis1a（而非 Pbx1b）的特异性合作转化原发性骨髓细胞。  |  Kroon, E., et al. 1998. EMBO J. 17: 3714-25. PMID: 9649441
  6. 同源框基因Hoxa-9的功能缺失会扰乱早期T细胞的发育并诱导原始胸腺细胞凋亡。  |  Izon, DJ., et al. 1998. Blood. 92: 383-93. PMID: 9657735

订购信息

产品名称产品编号规格价格数量收藏夹

HoxA9 抗体 (HOX5I043)

sc-81291
100 µg/ml
$333.00

HoxA9 (HOX5I043): m-IgG Fc BP-HRP 套装

sc-537242
100 µg Ab; 10 µg BP
$354.00

HoxA9 (HOX5I043): m-IgGκ BP-HRP 套装

sc-534465
100 µg Ab; 40 µg BP
$354.00

HoxA9 (HOX5I043): m-IgG1 BP-HRP 套装

sc-545098
100 µg Ab; 20 µg BP
$354.00

I would like to know the desired dilution of this HoxA9 antibody (sc-81291) used in Western Blot-based experiments to detect murine Hoxa9. Also, at what molecular weight can we expect to see the band for murine Hoxa9?

Asked by: Shagnik
Thank you for your question. As indicated on the product datasheet, HoxA9 (HOX5I043) is recommended for detection of HoxA9 of mouse, rat and human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000). The expected molecular weight of HoxA9 is 36 kDa.
Answered by: Tech Support Europe
Date published: 2022-06-22

Would it be possible to get a small FREE 10 µg sample of HoxA9 (HOX5I043): sc-81291 so I can test in WB if it is worth to buy it. We tried sc17155 and did not work.

Asked by: Shuchi
Thank you for your question. We would be happy to send you a sample of this antibody, sc-81291. Please contact our European Technical Service team and they will be glad to assist you. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-09-29

I am using HoxA9 (HOX5I043): sc-81291 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Randy McDonald
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Good for multiple applicationsThis antibody has been used for western blot and immunoperoxidase staining in published research. -SCBT QC
Date published: 2023-09-14
Rated 4 out of 5 by from DearI bouht it a mouth ago. I think it is very good.The concentration of Hoxa9 is 1:300.
Date published: 2017-07-05
Rated 3 out of 5 by from Positive results in WB using transfectedPositive results in WB using transfected 293T lysates and recombinant fusion protein. -SCBT QC
Date published: 2015-01-29
Rated 3 out of 5 by from Published western blot analysis of HoxA9Published western blot analysis of HoxA9 in U937 cell lysates. -SCBT Publication Review
Date published: 2014-04-11
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HoxA9 抗体 (HOX5I043) is rated 3.8 out of 5 by 4.
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