Date published: 2025-9-9
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Hog1 Antibody (D-3) - Western Blotting - Image 72905
Hog1 抗体 (D-3): sc-165978. 酵母重组 Hog1 融合蛋白的 Western 印迹分析.
Hog1 Antibody (D-3) - Western Blotting - Image 72905
Hog1 抗体 (D-3): sc-165978. 酵母重组 Hog1 融合蛋白的 Western 印迹分析.
Hog1 抗体 (D-3): sc-165978. 酵母重组 Hog1 融合蛋白的 Western 印迹分析.

Hog1 抗体 (D-3): sc-165978

2.6(7)
寫評論提問

说明书
  • Hog1 抗体 D-3 是小鼠单克隆 IgG1 κ，Hog1抗体, 在21篇文献中引用，规格为200 µg/ml
  • 免疫Saccharomyces cerevisiae物种的Hog1的氨基酸291-408
  • Hog1 抗体 (D-3) 推荐用于WB, IP, IF 和 ELISA，检测y 来源的 Hog1
  • 抗Hog1抗体（D-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Hog1 (D-3): sc-165978的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是Hog1 Antibody (D-3) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与Hog1 Antibody (D-3) 打包提供（请参阅下面的订购信息）。

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描述
说明书与实验方案
研究信息

関連項目

Hog1抗体（D-3）是一种IgG1κ小鼠单克隆Hog1抗体（也称为酵母高渗甘油（HOG）抗体、Hog1 MAPK抗体、蛋白激酶Hog1抗体或Hog1/丝裂原活化蛋白激酶抗体），可通过WB、IP、IF和ELISA检测酿酒酵母来源的Hog1蛋白。 Hog1抗体（D-3）既可以作为非偶联的抗Hog1抗体形式，也可以作为多种偶联形式的抗Hog1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。酵母细胞通过MAP激酶级联反应对环境作出反应，调节其内部渗透压。 MAP激酶级联反应将细胞外信号传递到细胞质或细胞核，是信号转导的重要组成部分。这些级联反应的核心由MAP激酶（丝裂原活化蛋白激酶，也称为ERK，用于胞外调节蛋白激酶）以及一个或多个上游调节激酶（MAPKK或MEK，用于MAP/ERK激酶）组成。高外渗导致MAPKK Pbs2的激活，从而激活MAP激酶Hog1。 Hog1（也称为Ssk3）被认为可以激活一种转录因子，上调渗透调节蛋白的产生。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Hog1 抗体 (D-3) 参考文献:

  1. PBS2 是一个编码假定蛋白激酶的酵母基因，它与 RAS2 通路相互作用并影响酿酒酵母的渗透敏感性。  |  Boguslawski, G. 1992. J Gen Microbiol. 138: 2425-32. PMID: 1479360
  2. Hog1通过RNA聚合酶II的重新分布和染色质重塑来绕过压力介导的转录下调。  |  Nadal-Ribelles, M., et al. 2012. Genome Biol. 13: R106. PMID: 23158682
  3. Hog1应激激活蛋白激酶通过靶向核孔蛋白来控制应激条件下的mRNA出口。  |  Regot, S., et al. 2013. J Biol Chem. 288: 17384-98. PMID: 23645671
  4. 应激活化蛋白激酶 Hog1 基于硫醇的直接威胁感应。  |  Guerra-Moreno, A., et al. 2019. Sci Signal. 12: PMID: 31772124
  5. 钙调素和 Hog1 的协调作用通过细胞周期网络的多个节点介导应激反应。  |  Leech, CM., et al. 2020. PLoS Genet. 16: e1008600. PMID: 32343701
  6. 酵母SAPKs Hog1和Mpk1之间的相互作用是由甘油积累介导的。  |  Laz, EV., et al. 2020. Fungal Biol. 124: 361-367. PMID: 32389298
  7. 在缺乏 Msn2, Msn4, Hot1 和 Sko1 的细胞中，Hog1 诱导的 RTC3 和 HSP12 转录非常活跃。  |  Bai, C., et al. 2020. PLoS One. 15: e0237540. PMID: 32804965
  8. HOG 途径通过酿酒酵母 CTT1 基因的应激反应元件 (STRE) 控制转录的渗透调节。  |  Schüller, C., et al. 1994. EMBO J. 13: 4382-9. PMID: 7523111
  9. 通过 MAPKKs 或结合含 SH3 的渗透传感器激活酵母 PBS2 MAPKK。  |  Maeda, T., et al. 1995. Science. 269: 554-8. PMID: 7624781
  10. 酵母中的渗透感应信号转导途径。  |  Brewster, JL., et al. 1993. Science. 259: 1760-3. PMID: 7681220
  11. 酵母中的 MAP 激酶通路：用于交配及更多。  |  Herskowitz, I. 1995. Cell. 80: 187-97. PMID: 7834739
  12. 酵母HOG1 MAP激酶级联受SLN1-YPD1-SSK1 '双组分 '渗透传感器中的多步磷酸循环机制调控。  |  Posas, F., et al. 1996. Cell. 86: 865-75. PMID: 8808622

订购信息

产品名称产品编号规格价格数量收藏夹

Hog1 抗体 (D-3)

sc-165978
200 µg/ml
$316.00

Hog1 (D-3): m-IgG Fc BP-HRP 套装

sc-528886
200 µg Ab; 10 µg BP
$354.00

Hog1 (D-3): m-IgGκ BP-HRP 套装

sc-521474
200 µg Ab, 40 µg BP
$354.00

Hog1 抗体 (D-3) AC

sc-165978 AC
500 µg/ml, 25% agarose
$416.00

Hog1 抗体 (D-3) HRP

sc-165978 HRP
200 µg/ml
$316.00

Hog1 抗体 (D-3) FITC

sc-165978 FITC
200 µg/ml
$330.00

Hog1 抗体 (D-3) PE

sc-165978 PE
200 µg/ml
$343.00

Hog1 抗体 (D-3) Alexa Fluor® 488

sc-165978 AF488
200 µg/ml
$357.00

Hog1 抗体 (D-3) Alexa Fluor® 546

sc-165978 AF546
200 µg/ml
$357.00

Hog1 抗体 (D-3) Alexa Fluor® 594

sc-165978 AF594
200 µg/ml
$357.00

Hog1 抗体 (D-3) Alexa Fluor® 647

sc-165978 AF647
200 µg/ml
$357.00

Hog1 抗体 (D-3) Alexa Fluor® 680

sc-165978 AF680
200 µg/ml
$357.00

Hog1 抗体 (D-3) Alexa Fluor® 790

sc-165978 AF790
200 µg/ml
$357.00

I would like to test the following antibodies in P. patoris: sc_166182 and sc_165978. Both should target Hog1 (phosphorylated and non-phosphorylated). Would it be possible to get some free samples? Many thanks and kind regards

Asked by: Anonymous
Thank you for your question. Please contact our technical service team directly to arrange for free antibody samples.
Answered by: Tech Support Europe
Date published: 2022-03-25

Is this antibody suitable to detect hog1 protein in Candida albicans? I am not getting any signal with this antibody.

Asked by: Farha
This antibody is raised against Hog1 of Saccharomyces cerevisiae origin. Please contact our Technical Service Department and we can help you BLAST the antigen against Candida albicans.
Answered by: Technical Support
Date published: 2018-12-11

For Western Blot, is it recommended to use denatured or non-denatured conditions with Hog1 (D-3): sc-165978 monoclonal antibody?

Asked by: Germaine
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from No bands recognizedI used this antibody against samples from a D273-10B strain and the antibodies did not recognize any bands on the Western blot.
Date published: 2025-06-27
Rated 1 out of 5 by from PoorI am using this antibody since may 2018. I am not getting any band on blots. Very poor quality antibody.
Date published: 2018-11-02
Rated 1 out of 5 by from poorI bought this antibody in july 2018. Still I am struggling with this antibody. I did almost all kind of troubleshootings but not able to get result. Now I am completely exhausted.
Date published: 2018-11-01
Rated 3 out of 5 by from No nos ha funcionadoLamentablemente no nos ha funcionado: la secuencia no parece tener el suficiente grado de homología con la proteína de la levadura con la que trabajamos. Quizá lo haría si se empleara el extremo amino, más conservado.
Date published: 2018-02-27
Rated 5 out of 5 by from Works great fo WBused at 1:2000, very specific on total cell lysates.
Date published: 2017-10-24
Rated 2 out of 5 by from I hope it can work!I got this product 4 mouths ago. First of all, I have to appreciate you for providing it for free. however, I am sorry to tell you that it didn't work in my western blotting experiment. My sample was from the cell extraction of candida albicans, and 40ug of proteins was loaded. After the incubations with antibodies, I used the Odyssey (LI-COR) to detect the hog1 protein, but unluckily, there was no band. In order to exan whether there exists the hog1 protein on the PVDF membrane, I incubate the membrnane with Ponceau S. As a result, I can see the red protein band there clearly. So I don't know whether there are any problems with your product. Hope your reply and suggestions. Thank you again!
Date published: 2017-09-15
Rated 1 out of 5 by from IHC Assay Review for Hog1 mABApplication: Immunohistochemistry. Protocol: Standard protocol for saliva smear staining. Dilutions tested: 1:10, 1:30, 1:90, 1:270. Results: No staining observed.
Date published: 2017-08-31
Rated 5 out of 5 by from Great Western blot data of yeast recombinantGreat Western blot data of yeast recombinant Hog1 fusion protein. -SCBT QC
Date published: 2013-04-15
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Hog1 抗体 (D-3) is rated 2.4 out of 5 by 8.
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