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m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for hnRNP F Antibody (3H4) for WB applications. These reagents are now offered in bundles with hnRNP F Antibody (3H4) (see ordering information below).
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hnRNP F Antibody (3H4) is a high quality monoclonal hnRNP F antibody (also designated HNRNPF antibody) suitable for the detection of the hnRNP F protein of human origin. hnRNP F Antibody (3H4) is available as both the non-conjugated anti-hnRNP F antibody form, as well as multiple conjugated forms of anti-hnRNP F antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to pre-mRNA processing and transport, and also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP complexes are the major constituents of the spliceosome and in particular, the hnRNP A1 protein is one of the major pre-mRNA/mRNA binding proteins and also one of the most abundant proteins in the nucleus. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. Most hnRNP proteins, including hnRNP C1 and C2, contain one or more RNA binding domains and are implicated in the processing of pre-mRNA. hnRNPs F and H are largely related factors that preferentially associate with poly(rG) regions on RNA. Isoforms of these proteins are often generated by alternative processing of the pre-mRNA and by posttranslational modifications such as phosphorylation on serines and threonines and methylation of arginines.
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What is the recommended fixation method for immunofluorescence staining with hnRNP F (3H4): sc-32309 monoclonal antibody?
Asked by: Dr Ninau Qelp
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: