hnRNP F抗体 (3H4) 推荐用于 human 来源的hnRNP F WB, IP, IF 和 ELISA检测

hnRNP F 抗体 (3H4): sc-32309

hnRNP F抗体 (3H4) is rated 4.3 out of 5 by 4.
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说明书

    • 抗-hnRNP F 抗体 3H4 是小鼠单克隆 IgG1 κ,hnRNP F抗体, 在3篇文献中引用,规格为200 µg/ml
    • raised against full length recombinant hnRNPF of human origin
    • hnRNP F 抗体 (3H4) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 hnRNP F
    • 抗-hnRNP F 抗体 (3H4) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取hnRNP F (3H4): sc-32309免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for hnRNP F Antibody (3H4) for WB applications. These reagents are now offered in bundles with hnRNP F Antibody (3H4) (see ordering information below).

What is the recommended fixation method for immunofluorescence staining with hnRNP F (3H4): sc-32309 monoclonal antibody?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Nice nuclear IF staining of HeLa cells We used this antibody for immunoflourescence staining of hnRNP-F. We were able to get nice signal intensity and images.
Date published: 2017-02-07
Rated 4 out of 5 by from Good specificity for WB in human hnRNP F Good specificity for WB in human hnRNP F transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-04-09
Rated 4 out of 5 by from Produced great immunofluorescence nuclear Produced great immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-15
Rated 4 out of 5 by from Good band in WB in K Good band in WB in K-562 nuclear extract. -SCBT QC
Date published: 2015-02-07
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