hnRNP F/H 抗体 (1G11): sc-32310
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- 抗-hnRNP F/H抗体(1G11)是小鼠单克隆IgG1 λ hnRNP F/H抗体, 在15篇文献中引用，规格为200 µg/ml
- raised against purified recombinant hnRNP F/H of human origin
- hnRNP F/H 抗体 (1G11) 推荐用于WB, IP, IF 和 IHC(P) ，检测 mouse, rat, human 和 Xenopus来源的hnRNP F and hnRNP H
- 抗-hnRNP F/H 抗体 (1G11) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
- 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
- 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
- 关于如何获取hnRNP F/H (1G11): sc-32310的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for hnRNP F/H Antibody (1G11) for WB and IHC(P) applications. This reagent is now offered in a bundle with hnRNP F/H Antibody (1G11) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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hello. i purified recombinant hnRNP-H, and reacted it with your antibody. but i always see 3 bends (~55kDa, ~40kDa, ~25kDa). i wanted to ask what is the epitope of the antibody, it may help me to understand the results. thank you Naomi
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Date published: 2020-03-03
I am using hnRNP F/H (1G11): sc-32310 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here:
https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Date published: 2017-02-24
Rated 5 out of
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Noriko from
WBに使用
WBにて使用した結果です.
条件は写真に記しています。
HEK293T細胞10cm 1枚分のタンパク量です。
Date published: 2020-11-04
Rated 5 out of
5
by
LE Khanh from
Excellent Antibody!
I received the free sample, and used for WB on DU145 lysate. I obtained a very satisfatory result, the bands were very strong, specific ! Love it so much :)
Highly recommend to you!
Date published: 2018-02-02
Rated 4 out of
5
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danni from
Good for WB
it works well for WB in our lab, not a bad choice.
Date published: 2017-09-18
Rated 5 out of
5
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AmYo from
Wonderful detection by WB
This antibody works in WB using human MSC lysate from different conditions.It detects both F/H isoforms clearly.
Date published: 2017-05-17
Rated 5 out of
5
by
SF from
Published data for WB in human epidermoid
Published data for WB in human epidermoid carcinoma A431 cells. -SCBT Publication Review
Date published: 2015-05-18
Rated 5 out of
5
by
SH from
Robust signal in WB in Jurkat and KNRK nuclear
Robust signal in WB in Jurkat and KNRK nuclear extracts. -SCBT QC
Date published: 2015-04-12
Rated 5 out of
5
by
ET from
Published data for WB in rat PC12 cells
Published data for WB in rat PC12 cells. -SCBT Publication Review
Date published: 2015-02-10
Rated 5 out of
5
by
JL from
Produced great immunoperoxidase nuclear staining
Produced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-01-28