hnRNP F/H抗体 (1G11) 推荐用于 mouse, rat, human 和 Xenopus 来源的hnRNP F and hnRNP H WB, IP, IF 和 IHC(P)检测

hnRNP F/H 抗体 (1G11): sc-32310

hnRNP F/H抗体 (1G11) is rated 4.9 out of 5 by 8.
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说明书

    • 抗-hnRNP F/H抗体(1G11)是小鼠单克隆IgG1 λ hnRNP F/H抗体, 在15篇文献中引用,规格为200 µg/ml
    • raised against purified recombinant hnRNP F/H of human origin
    • hnRNP F/H 抗体 (1G11) 推荐用于WB, IP, IF 和 IHC(P) ,检测 mouse, rat, human 和 Xenopus来源的hnRNP F and hnRNP H
    • 抗-hnRNP F/H 抗体 (1G11) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取hnRNP F/H (1G11): sc-32310免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for hnRNP F/H Antibody (1G11) for WB and IHC(P) applications. This reagent is now offered in a bundle with hnRNP F/H Antibody (1G11) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.

hello. i purified recombinant hnRNP-H, and reacted it with your antibody. but i always see 3 bends (~55kDa, ~40kDa, ~25kDa). i wanted to ask what is the epitope of the antibody, it may help me to understand the results. thank you Naomi

Asked by: Naomita613
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2020-03-03

I am using hnRNP F/H (1G11): sc-32310 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from WBに使用 WBにて使用した結果です. 条件は写真に記しています。 HEK293T細胞10cm 1枚分のタンパク量です。
Date published: 2020-11-04
Rated 5 out of 5 by from Excellent Antibody! I received the free sample, and used for WB on DU145 lysate. I obtained a very satisfatory result, the bands were very strong, specific ! Love it so much :) Highly recommend to you!
Date published: 2018-02-02
Rated 4 out of 5 by from Good for WB it works well for WB in our lab, not a bad choice.
Date published: 2017-09-18
Rated 5 out of 5 by from Wonderful detection by WB This antibody works in WB using human MSC lysate from different conditions.It detects both F/H isoforms clearly.
Date published: 2017-05-17
Rated 5 out of 5 by from Published data for WB in human epidermoid Published data for WB in human epidermoid carcinoma A431 cells. -SCBT Publication Review
Date published: 2015-05-18
Rated 5 out of 5 by from Robust signal in WB in Jurkat and KNRK nuclear Robust signal in WB in Jurkat and KNRK nuclear extracts. -SCBT QC
Date published: 2015-04-12
Rated 5 out of 5 by from Published data for WB in rat PC12 cells Published data for WB in rat PC12 cells. -SCBT Publication Review
Date published: 2015-02-10
Rated 5 out of 5 by from Produced great immunoperoxidase nuclear staining Produced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-01-28
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