Date published: 2025-9-11
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Hep B cAg Antibody (C1-5) - Western Blotting - Image 9592
乙型肝炎 HBcAg (C1-5): sc-23945. 人重组乙肝 HBcAg 的 Western 印迹分析. 注意到存在 14 kDa 倍数的乙肝 HBcAg 二聚体, 三聚体和四聚体.
Hep B cAg Antibody (C1-5) - Western Blotting - Image 9592
乙型肝炎 HBcAg (C1-5): sc-23945. 人重组乙肝 HBcAg 的 Western 印迹分析. 注意到存在 14 kDa 倍数的乙肝 HBcAg 二聚体, 三聚体和四聚体.
乙型肝炎 HBcAg (C1-5): sc-23945. 人重组乙肝 HBcAg 的 Western 印迹分析. 注意到存在 14 kDa 倍数的乙肝 HBcAg 二聚体, 三聚体和四聚体.

Hep B cAg 抗体 (C1-5): sc-23945

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寫評論提問

说明书
  • Hep B cAg 抗体 C1-5 是小鼠单克隆 IgG2a κ，Hep B cAg抗体, 在17篇文献中引用，规格为200 µg/ml
  • 针对Hep B cAg
  • Hep B cAg 抗体 (C1-5) 推荐用于WB, IP, IF, FCM 和 ELISA，检测Hep B 来源的 an epitope corresponding to amino acids 74-80 of the core antigen; 可能与 denatured Hep B cAg产生交联反应
  • 抗Hep B cAg抗体（C1-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Hep B cAg (C1-5): sc-23945的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是Hep B cAg Antibody (C1-5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Hep B cAg Antibody (C1-5) 打包提供（请参阅下面的订购信息）。

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说明书与实验方案
研究信息

関連項目

额外Hepatitis B Protein抗体，包括Hep B cAg, Hep B eAg, Hep B Pol, Hep B preS1, Hep B preS2, Hep B sAg和Hep B xAg

Hep B cAg 抗体（C1-5）是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）检测来自乙肝的 Hep B cAg 蛋白。Hep B cAg (C1-5) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。乙型肝炎病毒（HBV）属于肝病毒家族，以引起严重肝炎症而闻名，可导致呕吐、黄疸等症状，严重时甚至会导致死亡。核心抗原（Hep B cAg）在病毒生命周期中起着至关重要的作用，因为Hep B cAg包裹着病毒基因组，形成衣壳，在传播和感染过程中保护遗传物质。了解乙肝核心抗原的结构和功能至关重要，因为乙肝核心抗原不仅有助于诊断乙肝病毒感染，而且可以作为治疗干预的目标。乙肝核心抗原的存在，以及乙肝表面抗原（Hep B sAg）和e抗原（Hep B eAg）等其他病毒抗原的存在，对于监测病毒复制和评估抗病毒治疗的有效性至关重要。Hep B cAg和Hep B eAg主要用于临床诊断，而Hep B sAg对于疫苗开发和预防策略至关重要。这些抗原主要在肝脏中表达，这凸显了该器官在HBV发病机理中的核心作用，以及靶向疗法在治疗肝相关并发症中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Hep B cAg 抗体 (C1-5) 参考文献:

  1. 在 hu-PBL-NOD/SCID 小鼠模型中，HBcAg 特异性 CD4（+）Th1 型 T 细胞克隆抑制体内抗乙型肝炎病毒核心抗原（HBcAg）免疫球蛋白 G 的产生。  |  Cao, T., et al. 2001. J Virol. 75: 11449-56. PMID: 11689626
  2. 急性和慢性乙型肝炎患儿 CD4 T 淋巴细胞产生的 HBcAg 特异性细胞因子产物。  |  Szkaradkiewicz, A., et al. 2003. Virus Res. 97: 127-33. PMID: 14602204
  3. RNA 模板的暴露和剪接病毒 RNA 的封装受人类乙型肝炎病毒核心抗原（HBcAg 165-173）富含精氨酸结构域的影响。  |  Le Pogam, S., et al. 2005. J Virol. 79: 1871-87. PMID: 15650211
  4. 丙型肝炎病毒核心抗原检测：在诊断、疾病监测和治疗中的作用。  |  Tillmann, HL. 2014. World J Gastroenterol. 20: 6701-6. PMID: 24944462
  5. 定量乙型肝炎核心抗原抗体以预测HBV感染患者的结果。  |  Lim, YS. 2019. Clin Gastroenterol Hepatol. 17: 39-40. PMID: 30099105
  6. 乙型肝炎病毒自然发生的核心抗原变体的病毒体分泌。  |  Shih, C., et al. 2020. Cells. 10: PMID: 33396864
  7. 乙型肝炎核心抗原（HBcAg）病毒样颗粒和结合核酸的绝对定量。  |  Valentic, A., et al. 2023. Viruses. 16: PMID: 38275948
  8. 引入载有佐剂的颗粒状乙肝核心抗原作为乙肝疫苗的替代治疗成分。  |  Su, J., et al. 2024. JHEP Rep. 6: 100997. PMID: 38425450
  9. 乙型肝炎病毒变性核心蛋白抗体识别的表位。  |  Bichko, V., et al. 1993. Mol Immunol. 30: 221-31. PMID: 7679466
  10. 用单克隆抗体扫描鉴定暴露或内化在 HBcAg 颗粒表面的乙型肝炎病毒核心蛋白区域。  |  Pushko, P., et al. 1994. Virology. 202: 912-20. PMID: 8030252
  11. 乙型肝炎病毒核心抗原小鼠单克隆抗体可变区的结构。  |  Skrivelis, V., et al. 1993. Scand J Immunol. 37: 637-43. PMID: 8316761
  12. 使用单克隆抗体对受感染肝组织中的乙型肝炎病毒核心基因产物进行分化。  |  Naoumov, NV., et al. 1997. J Med Virol. 53: 127-38. PMID: 9334923

订购信息

产品名称产品编号规格价格数量收藏夹

Hep B cAg 抗体 (C1-5)

sc-23945
200 µg/ml
$316.00

Hep B cAg (C1-5): m-IgG Fc BP-HRP 套装

sc-525508
200 µg Ab; 10 µg BP
$354.00

Hep B cAg (C1-5): m-IgG2a BP-HRP 套装

sc-546295
200 µg Ab; 10 µg BP
$354.00

Hep B cAg 抗体 (C1-5) AC

sc-23945 AC
500 µg/ml, 25% agarose
$416.00

Hep B cAg 抗体 (C1-5) HRP

sc-23945 HRP
200 µg/ml
$316.00

Hep B cAg 抗体 (C1-5) FITC

sc-23945 FITC
200 µg/ml
$330.00

Hep B cAg 抗体 (C1-5) PE

sc-23945 PE
200 µg/ml
$343.00

Hep B cAg 抗体 (C1-5) Alexa Fluor® 488

sc-23945 AF488
200 µg/ml
$357.00

Hep B cAg 抗体 (C1-5) Alexa Fluor® 546

sc-23945 AF546
200 µg/ml
$357.00

Hep B cAg 抗体 (C1-5) Alexa Fluor® 594

sc-23945 AF594
200 µg/ml
$357.00

Hep B cAg 抗体 (C1-5) Alexa Fluor® 647

sc-23945 AF647
200 µg/ml
$357.00

Hep B cAg 抗体 (C1-5) Alexa Fluor® 680

sc-23945 AF680
200 µg/ml
$357.00

Hep B cAg 抗体 (C1-5) Alexa Fluor® 790

sc-23945 AF790
200 µg/ml
$357.00

Please tell me the protocol followed to run this western blot and reason for multimers of HBV core using Hep B cAg Antibody (C1-5): sc-23945. while with of HBV core Cat no. Hep B cAg antibody 10E11 : sc-23947 shows single band only.

Asked by: Pooja Bhatia
Thank you for contacting Santa Cruz Biotechnology. Here is our protocol : https://www.scbt.com/resources/protocols/western-immuno-blotting. If you would like to troubleshoot the antibody please email scbt@scbt.com or call (800)-457-3801.
Answered by: BlakeJ
Date published: 2024-10-11

do you have any examples of this antibody being used for western blotting on HBV-infected cells?

Asked by: Carlos I
Thank you for your question. We do not have any data or publications for HBV infected cells.
Answered by: BlakeJ
Date published: 2024-04-17

I am considering this antibody for my assay study, I need to know is the example WB picture done in reducing condition or non-reducing condition? Does this antibody recognize the epitope in both reducing and non-reducing condition? Thanks！

Asked by: ironbear
Thank you for your inquiry. This antibody was tested in house on reducing conditions. We have not tested it in non-reducing conditions, but it is very possible that it will detect the epitope under non-reducing conditions as well. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2024-01-02

I am going to run a Western blot with this antibody, what is the expected molecular weight?

Asked by: TinTin
The expected molecular weight for Hep B cAg is 21kDa, however it has been reported that this protein may be able to form dimers, trimers or even tetramers. Our in house testing on this antibody on recombinant protein shows potential multimers, however we do not have information regarding endogenous samples.
Answered by: Technical Support 13
Date published: 2017-01-26

What secondary is best to use with Hep B cAg Antibody (C1-5): sc-23945?

Asked by: jenni
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with our Hep B cAg (C1-5): sc-23945, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-09
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Rated 5 out of 5 by from good antibodyGood antibody that produces strong signal in western blot. Also recommended for flow cytometry!
Date published: 2017-01-12
Rated 4 out of 5 by from Publishable data in ChIP using human liverPublishable data in ChIP using human liver. -SCBT Publication Review
Date published: 2015-03-27
Rated 3 out of 5 by from Bands detected in WB using human recombinantBands detected in WB using human recombinant Hep B HBcAg. -SCBT QC
Date published: 2015-03-27
Rated 4 out of 5 by from Publishable data in CoIP using human Huh7 cellsPublishable data in CoIP using human Huh7 cells. -SCBT Publication Review
Date published: 2015-03-22
Rated 4 out of 5 by from Various publishable data in WB using humanVarious publishable data in WB using human Huh7 cells and Foreskin t-fected with HPV16, 18 -SCBT Publication Review
Date published: 2014-11-11
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Hep B cAg 抗体 (C1-5) is rated 4.0 out of 5 by 5.
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