Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Heme Oxygenase 2 CRISPR/Cas9 KO Plasmid (m): sc-420883
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Heme Oxygenase 2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Heme Oxygenase 2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Heme Oxygenase 2 Antibody (B-3): sc-17786
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Heme Oxygenase 2 CRISPR/Cas9 KO Plasmid (m) | sc-420883 | 20 µg | $397.00 |
Overview
Hmox2 encodes heme oxygenase 2 (HO-2), a constitutively expressed, endoplasmic reticulum–associated enzyme that catalyzes heme degradation to biliverdin, free iron, and carbon monoxide. Through regulation of cellular heme availability and generation of redox-active metabolites, HO-2 influences oxidative stress responses, iron homeostasis, and carbon monoxide–mediated signaling that can modulate vascular tone and neuronal activity. In mouse tissues, Hmox2 is prominent in the nervous system and contributes to metabolic adaptation, protection from heme-mediated cytotoxicity, and inflammatory signaling crosstalk. Dysregulated heme turnover and altered HO-2 activity have been implicated in models of neuroinflammation, ischemic stress, and cardiopulmonary physiology, making Hmox2 a useful target for pathway-level studies of heme metabolism and stress signaling.
Heme Oxygenase 2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hmox2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hmox2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hmox2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Heme Oxygenase 2 protein expression.
This CRISPR knockout system enables efficient generation of Hmox2-deficient cell models for investigation of Heme Oxygenase 2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hmox2 exon(s) critical for Heme Oxygenase 2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hmox2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Heme Oxygenase 2 CRISPR/Cas9 KO Plasmid (m) and Heme Oxygenase 2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hmox2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Heme Oxygenase 2 HDR Plasmid (m) and Heme Oxygenase 2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hmox2 homology arms to support homology-directed repair at defined Hmox2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.