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Histone Deacetylase 8 (HDAC8) Antibody (E-5) - Western Blotting - Image 1418
HDAC8 抗体 (E-5): sc-17778. K-562 核提取物中 HDAC8 表达的 Western 印迹分析.
HDAC8 抗体 (E-5): sc-17778. K-562 核提取物中 HDAC8 表达的 Western 印迹分析.

Histone Deacetylase 8 (HDAC8) 抗体 (E-5): sc-17778

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寫評論提問

说明书
  • Histone Deacetylase 8 (HDAC8) 抗体 E-5 是小鼠单克隆 IgG2b κ，Histone Deacetylase 8 (HDAC8)抗体, 在16篇文献中引用，规格为200 µg/ml
  • 免疫human物种的HDAC8的氨基酸1-145
  • Histone Deacetylase 8 (HDAC8) 抗体 (E-5) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 HDAC8
  • 抗Histone Deacetylase 8 (HDAC8)抗体（E-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Histone Deacetylase 8 (HDAC8) (E-5): sc-17778的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Histone Deacetylase 8 (HDAC8) 抗体 (E-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Histone Deacetylase 8 (HDAC8) 抗体 (E-5) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 HDAC 抗体 包括 HDAC1, HDAC10, HDAC11, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7和HDAC8

HDAC8抗体（E-5）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）和酶联免疫吸附测定（ELISA）检测人类HDAC8。HDAC8 (E-5) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。HDAC8 是组蛋白去乙酰化酶家族的一员，主要位于细胞核中，通过修饰染色质结构在基因表达调控中发挥关键作用。HDAC8通过去除组蛋白上赖氨酸残基的乙酰基，促进染色质的凝结，从而抑制转录。这一过程对于维持细胞内稳态和调节发育过程至关重要，因为异常的HDAC活性与多种癌症和其他疾病有关。HDAC8与多种其他蛋白质相互作用，包括转录因子和共抑制因子，从而进一步调节基因调控。了解HDAC8的功能和相互作用对于阐明其对细胞过程的贡献以及作为癌症治疗潜在治疗靶点的可能性至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Histone Deacetylase 8 (HDAC8) 抗体 (E-5) 参考文献:

  1. 作为转录抑制因子发挥作用的新型人类 I 类组蛋白去乙酰化酶的克隆和表征。  |  Hu, E., et al. 2000. J Biol Chem. 275: 15254-64. PMID: 10748112
  2. 组蛋白去乙酰化酶 8 是一种 I 类组蛋白去乙酰化酶，其表达仅限于正常人体组织中出现平滑肌分化的细胞。  |  Waltregny, D., et al. 2004. Am J Pathol. 165: 553-64. PMID: 15277229
  3. 一种选择性 HDAC8 抑制剂能增强肝细胞癌的抗肿瘤免疫力和免疫检查点阻断的疗效。  |  Yang, W., et al. 2021. Sci Transl Med. 13: PMID: 33827976
  4. 口蹄疫病毒结构蛋白VP3与HDAC8相互作用，促进其自噬降解，从而促进病毒复制。  |  Zhang, H., et al. 2023. Autophagy. 19: 2869-2883. PMID: 37408174
  5. HDAC3和HDAC8 PROTAC双降解剂揭示了组蛋白乙酰化在基因调控中的作用。  |  Xiao, Y., et al. 2023. Cell Chem Biol. 30: 1421-1435.e12. PMID: 37572669
  6. 乙酰化导致的核小体结构变化  |  Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
  7. 组蛋白乙酰化在转录因子进入核糖体 DNA 的过程中发挥了积极作用。  |  Lee, DY., et al. 1993. Cell. 72: 73-84. PMID: 8422685
  8. 酵母的转录沉默与核小体乙酰化减少有关。  |  Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
  9. 核糖体 DNA 调节人类 Hat1 乙酰转移酶的核心-活素结合亚基。  |  Verreault, A., et al. 1998. Curr Biol. 8: 96-108. PMID: 9427644
  10. 转录激活剂将组蛋白乙酰转移酶复合物导向核糖体。  |  Utley, RT., et al. 1998. Nature. 394: 498-502. PMID: 9697775
  11. 人类 HDAC8 的结构快照提供了对 I 类组蛋白去乙酰化酶的深入了解。  |  Somoza, JR., et al. 2004. Structure. 12: 1325-34. PMID: 15242608
  12. 组蛋白去乙酰化酶 HDAC8 与平滑肌α-肌动蛋白结合，对平滑肌细胞的收缩能力至关重要。  |  Waltregny, D., et al. 2005. FASEB J. 19: 966-8. PMID: 15772115

订购信息

产品名称产品编号规格价格数量收藏夹

Histone Deacetylase 8 抗体 (E-5)

sc-17778
200 µg/ml
$316.00

Histone Deacetylase 8 (HDAC8) (E-5): m-IgGκ BP-HRP 套装

sc-520650
200 µg Ab, 40 µg BP
$354.00

Histone Deacetylase 8 (HDAC8) (E-5): m-IgG2b BP-HRP 套装

sc-548844
200 µg Ab; 10 µg BP
$354.00

Histone Deacetylase 8 (HDAC8) 抗体 (E-5) AC

sc-17778 AC
500 µg/ml, 25% agarose
$416.00

Histone Deacetylase 8 (HDAC8) 抗体 (E-5) HRP

sc-17778 HRP
200 µg/ml
$316.00

Histone Deacetylase 8 (HDAC8) 抗体 (E-5) FITC

sc-17778 FITC
200 µg/ml
$330.00

Histone Deacetylase 8 (HDAC8) 抗体 (E-5) PE

sc-17778 PE
200 µg/ml
$343.00

Histone Deacetylase 8 (HDAC8) 抗体 (E-5) Alexa Fluor® 488

sc-17778 AF488
200 µg/ml
$357.00

Histone Deacetylase 8 (HDAC8) 抗体 (E-5) Alexa Fluor® 546

sc-17778 AF546
200 µg/ml
$357.00

Histone Deacetylase 8 (HDAC8) 抗体 (E-5) Alexa Fluor® 594

sc-17778 AF594
200 µg/ml
$357.00

Histone Deacetylase 8 (HDAC8) 抗体 (E-5) Alexa Fluor® 647

sc-17778 AF647
200 µg/ml
$357.00

Histone Deacetylase 8 (HDAC8) 抗体 (E-5) Alexa Fluor® 680

sc-17778 AF680
200 µg/ml
$357.00

Histone Deacetylase 8 (HDAC8) 抗体 (E-5) Alexa Fluor® 790

sc-17778 AF790
200 µg/ml
$357.00

Hi, I am not sure how I can fix this, but above my review said: "Also detects a smaller band < 25kDa", this is incorrect. This is a copy paste error from my another review for another antibody. Could you please delete that sentence?

Asked by: gulg_genetic
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Can HDAC8 (E-5): sc-17778 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, HDAC8 (E-5): sc-17778 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from Weak signalAlso detects a smaller band < 25kDa Using human blood monocyte derived macrophages, 25 ug total cell lysate ran on midi gels, 1:500 dilution in milk, 1:4000 2nd Ab CST anti-mouse we could detect a weak band at right size using lightning ecl.
Date published: 2021-07-19
Rated 2 out of 5 by from not work well for my experimentwe repeated the experiment more than three times and we did not detect the band on the predict position (1:500, rat heart extrat)
Date published: 2017-09-21
Rated 5 out of 5 by from High QualityThe HDAC8 Antibody (E-5): sc-17778 works very well for immunofluorescence applications, showing strong staining with nuclear localization. This antibody has also been cited multiple times for further verification.
Date published: 2017-02-08
Rated 5 out of 5 by from Good antibody for Immunofluorescence stainingGood antibody for Immunofluorescence staining of methanol-fixed MOLT-4 cells showing nuclear localization. - SCBT QC
Date published: 2015-04-27
Rated 5 out of 5 by from Clean blot with single band in human HDAC8Clean blot with single band in human HDAC8 transfected 293T whole cell lysates by WB. - SCBT QC
Date published: 2015-04-15
Rated 4 out of 5 by from Weak bands in nonWeak bands in non-transfected control and HDAC8 siRNA transfected HeLa cells. - SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Single strong band with a little backgroundSingle strong band with a little background for western blot analysis of HDAC8 expression in K-562 nuclear extract. - SCBT QC
Date published: 2013-03-15
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Histone Deacetylase 8 (HDAC8) 抗体 (E-5) is rated 4.0 out of 5 by 7.
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