HAS3抗体 (G-12) 推荐用于 human 来源的HAS3 WB, IP, IF, IHC(P) 和 ELISA检测

HAS3 抗体 (G-12): sc-365322

HAS3抗体 (G-12) is rated 4.7 out of 5 by 6.
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说明书

    • 抗-HAS3 抗体 G-12 是小鼠单克隆 IgG2b (kappa轻链),HAS3抗体,规格为200 µg/ml
    • raised against amino acids 124-187 mapping within an internal region of HAS3 of human origin
    • HAS3 抗体 (G-12) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 HAS3; 也和以下物种反应,包括: bovine
    • 抗-HAS3 抗体 (G-12) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取HAS3 (G-12): sc-365322免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 HAS3 Antibody (G-12) for WB and IHC(P) 首选应用二抗. 这些试剂现在以套形式提供 HAS3 Antibody (G-12) (见下方订购信息).
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Hi, have you tested HAS3 (G-12) sc-365322 for the use in flow cytometry and if so, are there any protocols you can recommend? Thank you very much!

Asked by: Christian Hundhausen
Thank you for your question. We recommend the conjugates with Alexa Fluor® 488, 546, 594, and 647 of HAS3 (G-12): sc-365322 for use in flow cytometry. You can find our recommended protocol for flow cytometry by following this link: https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: SCBT Heidelberg Support
Date published: 2018-12-22

I am using HAS3 (G-12): sc-365322 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Ottimo Funzionamento! Ottimo funzionamento. Testato su rene umano alla condizione 1:50 ph6
Date published: 2020-11-19
Rated 3 out of 5 by from This antibody does not work in zebrafish I tried with 2%PFA fixation and acetone permeabilization, does not work on 2 days old fish
Date published: 2018-02-26
Rated 5 out of 5 by from Good for IF Good signal for IF, not a lot of background - used on MDA-MB-231 cells
Date published: 2017-08-17
Rated 5 out of 5 by from Strong Signal, Good for WB This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including bovine and human samples.
Date published: 2017-02-07
Rated 5 out of 5 by from Works great for human HAS3 tranfected lysates Works great for human HAS3 tranfected lysates. -SCBT QC
Date published: 2015-06-14
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic staining Superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-03-07
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